Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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The Cultural Characteristics and Antibacterial Activities of Cordyceps militaris and Paecilomyces tenuipes

번데기동충하초와 눈꽃동충하초의 배양특성 및 항균활성

  • Lee, Ki-Man (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hong, In-Pyo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Sung, Gyoo-Byung (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Bae, Yoon-Hwan (Department of Life Science, College of Natural Science, Daejin University)
  • 이기만 (국립농업과학원 농업생물부) ;
  • 홍인표 (국립농업과학원 농업생물부) ;
  • 남성희 (국립농업과학원 농업생물부) ;
  • 성규병 (국립농업과학원 농업생물부) ;
  • 배윤환 (대진대학교 자연과학대학 생명과학과)
  • Published : 2008.12.30
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Abstract

The cultural characteristics and antibacterial activities of Cordyceps militaris and Paecilomyces tenuipes were compared. The mycelial growth was the highest on MCM (Mushroom Complete Medium) for C. militaris and on YMA (Yeast Malt Agar) for P. tenuipes. But the mycelial density on MMM (Mushroom Minimal Medium) was lower than other on media. The optimum mycelial growth was observed at $25^{\circ}C$. C. militaris was low mycelial growth when it was transferred over 5 times generation. The carbon source for the optimum mycelial growth was fructose of monosaccharide, maltose of disaccharide and dextrin of polysaccharide. The calcium nitrate of organonitrogen was found the best mycelial growth on C. militaris, while the sodium nitrate observed to be well for mycelial growth on P. tenuipes. The ammonium tartrate was observed to be the best among the inorganonitrogen used for mycelial growth. Antibacterial activities were found out just C. militaris against Bacillus cereus of Gram (+).

Cordyceps militaris(번데기동충하초)와 Paecilomyces tenuipes(눈꽃동충하초)의 배양특성 및 항균활성을 비교하였다. 균사 생장 최적 배지는 C. militaris는 MCM, P. tenuipes는 YMA이며 MMM 배지 상에서는 두 균주 모두 생장이 저조하였다. 균사 생육 최적 온도는 $25^{\circ}C$이였고 C. militaris는 5회 이상 계대 시 균사 생육이 저하되었다. 적정 영양원 선발에 있어 두 균주 모두 탄소원은 단당류의 fructose, 이당류의 maltose 그리고 다당류의 dextrin이 적합하였다. 질소원은 유기태에서 C. militaris는 calcium nitrate, P. tenuipes는 sodium nitrate가 적합하였으며 무기태에서는 ammonium tartrate가 적합하였다. 항균활성은 Gram(+)의 Bacillus cereus에 대하여 C. militaris에서만 나타났다.

Keywords

References

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