Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Characterization of Bacillus thuringiensis subsp. tohokuensis CAB167 Isolate against Mosquito Larva

모기유충에 활성 있는 Bacillus thuringiensis subsp. tohokuensis CAB167 균주의 특성

  • Kil, Mi-Ra (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Da-A (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Paek, Seung-Kyoung (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Jin-Su (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Choi, Su-Yeon (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Jin, Da-Yong (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Youn, Young-Nam (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Hwang, In-Chon (Central Research Institute, Kyung Nong Co.) ;
  • Ohba, Michio (Bioresources and Management Laboratory, Graduate School of Agriculture, Kyushu University) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University)
  • 길미라 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김다아 (충남대학교 농업생명과학대학 응용생물과) ;
  • 백승경 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김진수 (충남대학교 농업생명과학대학 응용생물과) ;
  • 최수연 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김대용 (충남대학교 농업생명과학대학 응용생물과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물과) ;
  • 황인천 ((주) 경농 중앙연구소) ;
  • ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물과)
  • Published : 2008.12.30
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Abstract

Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. $LC_{50}$ values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.

본 연구는 모기에 활성을 나타내는 Bacillus thuringiensis subsp. tohokuensis CAB167균주에서 생산된 내독소결정단백질의 특성을 조사하였다. B. thuringiensis subsp. tohokuensis CAB167균주는 위상차 현미경과 주사전자현미경으로 관찰하여 일반적으로 모기에 활성을 나타내는 spherical type의 내독소결정 성단백질을 형성하는 것으로 나타났다. 국내에서 서식하는 지하집모기(Culex pipiens molestus), 빨간집모기(Culex pipiens pallens), 이집트숲모기(Aedes aegyti)에 대한 이 균주의 살충활성검정은 각각 173, 190, 580 ng/ml의 $LC_{50}$ 값으로 각각 활성을 보였다. B. thuringiensis subsp. tohokuensis CAB167균주에서 생산된 내독소결정성단백질의 SDS-PAGE를 통해 135, 80, 49와 28-kDa의 주요한 4개의 밴드를 나타냈다. 내독소결정성단백질의 소화에 영향을 미치는 곤충중장액과 유사한 trypsin효소를 처리하여 72와 63-kDa의 단백질이 새롭게 생성하였다. 파리목 위생해충인 모기에 활성을 나타내는 다른 7개의 B. thuringiensis 균주들의 내독소 결정성단백질과 혈청학적인 비교실험에서 독소단백질이 차이가 있는 것을 확인하였다.

Keywords

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