한국응용곤충학회지 (Korean journal of applied entomology)

  • 제47권4호
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  • Pages.435-445
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  • 2008
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
권호 표지
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고추를 가해하는 담배나방[Helicoverpa assulta (Guenée)]의 효과적 방제를 위한 쌀좀알벌(Trichogramma evanescens Westwood)과 미생물제제의 종합생물방제 효과

Efficacy of an Integrated Biological Control of an Egg Parasitoid, Trichogramma evanescens Westwood, and Microbial Insecticide Against the Oriental Tobacco Budworm, Helicoverpa assulta (Guenée) Infesting Hot Pepper

  • 김근섭 (안동대학교 생명자원과학과) ;
  • 허혜정 (안동대학교 생명자원과학과) ;
  • 박정아 (안동대학교 생명자원과학과) ;
  • 유용석 ((주) 나비스 중앙연구소) ;
  • 함은혜 ((주) 나비스 중앙연구소) ;
  • 강성영 ((주) 나비스 중앙연구소) ;
  • 권기면 ((주) 나비스 중앙연구소) ;
  • 이건형 ((주) 나비스 중앙연구소) ;
  • 김용균 (안동대학교 생명자원과학과)
  • Kim, Geun-Seob (Department of Bioresource Sciences, Andong National University) ;
  • Heo, Hye-Jung (Department of Bioresource Sciences, Andong National University) ;
  • Park, Jung-A (Department of Bioresource Sciences, Andong National University) ;
  • Yu, Yong-Suk (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Hahm, Eun-Hye (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kang, Sung-Young (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kwon, Ki-Myeon (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Lee, Keon-Hyung (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 발행 : 2008.12.30
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초록

고추를 가해하는 담배나방(Helicoverpa assulta ($Guen\acute{e}e$))의 최적 방제 적기는 과실 내부로 침투하기 이전 시기인 알과 어린 유충으로 인식되고 있다. 본 연구는 담배나방의 이러한 감수성 발육시기를 대상으로 하는 효과적 생물적 방제 기술을 개발하는 데 목표를 두었다. 쌀좀알벌(Trichogramma evanescens Westwood)은 알기생봉으로 담배나방 알도 기생시키는 것이 본 연구를 통해 관찰되었다. 그람양성균인 토양 곤충병원세균(Bacillus thuringiensis subsp. kurstaki)과 그람음성균인 곤충병원세균(Xenorhabdus nematophila ANU101)은 어린 담배나방 유충을 대상으로 상호 협력적 병원력을 발휘하였다. 고추 포장의 담배나방 성충 발생은 페로몬트랩을 통해 모니터링하였다. 혼합 미생물제제는 접촉과 섭식 독성 분석 모두에서 쌀좀알벌의 유충 발육과 성충 생존에 영향을 주지 않는 것으로 판명되었다. 담배나방 성충 발생이 성페로몬 트랩으로 확인된 시기에 고추포장에 쌀좀알벌과 미생물제제의 혼합 처리가 실시되었다. 혼합 생물방제 처리는 화학농약과 비슷한 수준으로 과실의 피해를 현저히 감소시킨 반면, 단독 생물방제 처리는 효과가 현저히 낮았다. 아울러 본 연구는 쌀좀알벌의 형태적 및 유전적 특징을 분석했다.

Due to internal feeding behavior, the oriental tobacco budworm, Helicoverpa assulta ($Guen\acute{e}e$), infesting hot pepper has been regarded to be effectively controlled by targeting egg and neonate larval stages just before entering the fruits. This study aimed to develop an efficient biological control method focusing on these susceptible stages of H. assulta. An egg parasitoid wasp, Trichogramma evanescens Westwood, was confirmed to parasitize the eggs of H. assulta. A mixture of Gram-positive soil bacterium, Bacillus thuringiensis subsp. kurstaki, and Gram-negative entomopathogenic bacterium, Xenorhabdus nematophila ANU101, could effectively kill neonate larvae of H. assulta. A sex pheromone trap monitored the occurrence of field H. assulta adults. The microbial insecticide mixture was proved to give no detrimental effects on immature development and adult survival of the wasp by both feeding and contact toxicity tests. A combined treatment of egg parasitoid and microbial pesticide was applied to hot pepper fields infested by H. assulta. The mixture treatment of both biological control agents significantly decreased the fruit damage, which was comparable to the chemical insecticide treatment, though either single biological control agent did not show any significant control efficacy. This study also provides morphological and genetic characters of T. evanescens.

키워드

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피인용 문헌

  1. Developmental Performance and Parasitism of Trichogramma chilonis Ishii on Eggs of the Oriental Tobacco Budworm, Helicoverpa assulta(Guenee), and the American Bollworm, Helicoverpa armigera (Hubner) Depending on Previous Hosts vol.49, pp.4, 2010, https://doi.org/10.5656/KSAE.2010.49.4.393