Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of Korean Mistletoe (Viscum album coloratum) Extract (KME) on the Extensions of Life span in Silkworm Moth, Bombyx Mori

누에 나방에 있어서 한국산 겨우살이(Viscum album coloratum) 추출물의 생명연장 효과

  • Jung, Hoe-Yune (School of Life and Food Sciences, Handong Global University) ;
  • Lee, An-Na (School of Life and Food Sciences, Handong Global University) ;
  • Kim, Sae-Jung (School of Life and Food Sciences, Handong Global University) ;
  • Park, Sung-Min (School of Life and Food Sciences, Handong Global University) ;
  • Song, Tae-Jun (School of Life and Food Sciences, Handong Global University) ;
  • Ko, Byung-Woong (School of Life and Food Sciences, Handong Global University) ;
  • Kim, Young-Hoon (School of Life and Food Sciences, Handong Global University) ;
  • An, Hyo-Sun (School of Life and Food Sciences, Handong Global University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Min, Byeng-Ryel (Centro Educativo Ichthus) ;
  • Kim, Jong-Bae (School of Life and Food Sciences, Handong Global University)
  • 정회윤 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 이안나 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 김세중 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 박성민 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 송태준 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 고병웅 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 김영훈 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 안효선 (한동대학교 생명식품과학부 생명과학 연구실) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실) ;
  • 민병렬 (익투스 교육센터) ;
  • 김종배 (한동대학교 생명식품과학부 생명과학 연구실)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Viscum album coloratum (Korean mistletoe; KM) is a semi-parasitic plant growing on various trees. This plant hasbeen shown to possess a variety of biological functions such as immunomodulation, apoptosis-induction and antitumor activity. However, there is no information about how the extract of KM affects life span of the host. In this study, in order to examine the effect of KM on the longevity of the host, we investigated whether a Korean mistletoe extract (KME) was able to extend the life length in an experimental model using the spring silkworm moth of Bombyx mori (Lepidoptera bombycidae). We established a model test for survival in which all of the male and female moths died within 18 days after the beginning of the experiment. Mean survival time of female moths was longer than that of male moths. Both male and female moths fed with KME containing diet (200 or 400 ${\mu}g$/head/day) showed significantly higher mean survival times than those of the control moths. Studying the effect of KME on prolongation of mean survival time showed that male moths were more susceptible than female moths. The moths fed with KME-containing diet showed a slight decrease in body weight. Interestingly, however, no difference in food intake was observed between moths fed with KME-containing diet and those with control diet. In analysis for mRNA increase in the SIRT2 gene, a member of the Sir2 gene family playing important roles in regulation of cell death and prolongation of life span, moths fed with KME-containing diet showed a significant increase in SIRT2 gene expression. These results suggest that KME has a potential to extend the life span in Bombyx mori, and its effect is partly associated with increase in SIRT2 gene expression.

Keywords

References

  1. Stivala, L. A., Savio, M., Carafoli, F., Perucca, P., Bianchi, L., Maga, G., Forti, L., Pagnoni, U. M., Albini, A., Prosperi, E. and Vannini, V. (2001) Specific structural determinants are responsible for the antioxidant activity and the cell cycle effects of resveratrol. J. Biol. Chem., 276: 22586-22594 https://doi.org/10.1074/jbc.M101846200
  2. Valenzano, D. R., Terzibasi, E., Genade, T., Cattaneo, A., Domenici, L. and Cellerino, A. (2006) Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Curr. Biol., 16: 296-300 https://doi.org/10.1016/j.cub.2005.12.038
  3. Baur, J. A., Pearson, K. J., Price, N. L., Jamieson, H. A., Lerin, C., Kalra, A., Prabhu, V. V., Allard, J. S., Lopez-Lluch, G., Lewis, K., Pistell, P. J., Poosala, S., Becker, K. G., Boss, O., Gwinn, D., Wang, M., Ramaswamy, S., Fishbein, K. W., Spencer, R. G., Lakatta, E. G., Le couteur, D., Shaw, R. J., Navas, P., Puigserver, P., Ingram, D. K., deCabo, R. and Sinclair, D. A. (2006) Resveratrol improves health and survival of mice on a high-calorie diet. Nature, 444: 337-342 https://doi.org/10.1038/nature05354
  4. Zang, M., Xu, S., Maitland-Toolan, K. A., Zuccollo, A., Hou, X., Jiang, B., Wierzbicki, M., Verbeuren, T. J. and Cohen, R. A. (2006) Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes, 55: 2180-2191 https://doi.org/10.2337/db05-1188
  5. Guarente, L. (2000) Sir2 links chromatin silencing, metabolism, and aging. Genes Dev., 14: 1021-1026
  6. Marie, L., Carmen,. A., Zachary, G., Hamid, M., Carles, L., Frederic, D., Nadia, M., Jill, M., Philip, L., Peter, E., Bernard, G., Marllu, L., Pere, P. and Johan, A. (2006) Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1$\alpha$. Cell, 127: 1109-1122 https://doi.org/10.1016/j.cell.2006.11.013
  7. Blander, G. and Guarente, L. (2004) The Sir2 family of protein deacetylases. Annu. Rev. Biochem., 73: 417-435 https://doi.org/10.1146/annurev.biochem.73.011303.073651
  8. Rodgers, J. T., Lerin, C., Haas, W., Gygi, S. P., Spiegelman, B. M. and Puigserver, P. (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature, 434: 113-118 https://doi.org/10.1038/nature03354
  9. Sinclair, D.A. (2005) Toward a unified theory of caloric restriction and longevity regulation. Mech. Ageing Dev., 126: 987-1002 https://doi.org/10.1016/j.mad.2005.03.019
  10. Ryu, K. S., Lee, H. S. and Kim, Y. S. (1999). Pharmaco-dynamic study of power in mice administered to maltose, sucrose and lactose. Korean J. Seric. Sci., 41: 9-13
  11. Choi, J. H. and Kim, D. I. (1999) Effects of silkworm powder on oxygen radicals and their scavenger enzyme in serum of rats. Korean J. Seric. Sci., 41: 141-146
  12. Murakami, A., Kuroda, Y. and Fukase, Y. (1986) Sex and strain differences in the life span of the adult silkmoth, Bombyx mori. Ann. Rept. Natl. Icst. Genetics (Japan), 36: 49-50
  13. Murakami, A. (1989) Genetic studies on the silkworm adult lifespan heredity of the short adult lifespan (sdi). Ann, Rept. Natl. Inst. Genetics (Japan), 39: 67-68
  14. Rentea, R., Lyon, E. and Hunter, R. (1981) Biologic properties of Iscador: A Viscum album preparation: I. Hyperplasia of the thymic cortex and accelerated regeneration of hemato-poietic cells following X-irradiation. Lab Invest., 44: 43-48
  15. Yoon, T. J., Yoo, Y. C., Hong, E. K., Cho, Y. H., Lee, S. W., Azuma, I., Yoo, B. L. and Kim, J. B. (1994) Effect of Korean mistletoe extracts on the induction of IL-1 and TNF-$\alpha$ from mouse macrophage. Kor. J. Phamacogn., 25: 132-139
  16. Yoon, T. J., Yoo, Y. C., Choi, O. B., Do, M. S., Kang, T. B., Lee, S. W., Azuma, I. and Kim, J. B. (1995) Inhibitory effect of Korean mistletoe (Viscum album coloratum) extract on tumour angiogenesis and metastasis of haematogenous and non-haematogenous tumour cells in mice. Cancer letter, 97: 83-91 https://doi.org/10.1016/0304-3835(95)03956-W
  17. Song, S. K., Moldoveanu, Z., Nguyen, H. H., Kim, E. H., Choi, K. Y., Kim, J. B. and Mestecky, J. (2007) Intranasal immunization with influenza virus and Korean mistletoe lectin C (KML-C) induces heterosubtypic immunity in mice. Vaccine, 25: 6359-6366 https://doi.org/10.1016/j.vaccine.2007.06.030
  18. Yao, H., Liao, Z. X., Wu, Q., Lei, G. Q., Liu, Z. J., Chen, D. F., Chen, J. K. and Zhou, T. S. (2006) Antioxdative flavanone glycosides from the branches and leaves of Viscum coloratum. Chem. Pharm. Bull., 54: 133-135 https://doi.org/10.1248/cpb.54.133
  19. Gray, A. M. and Flatt, E. R. (1999) Insulin-secreting activity of the traditional antidiabetic plant Viscum album (mistletoe). J. endocrinol., 160: 409-414 https://doi.org/10.1677/joe.0.1600409
  20. Pallas, M., Verdaguer, E., Tajes, M., Gutierrez-Cuesta, J. and Camins, A. (2008) Modulation of sirtuins: new targets for antiageing. Recent Patents CNS Drug Discov., 3: 61-69 https://doi.org/10.2174/157488908783421492
  21. Wood, J., Rogina, B., Lavu, S., Howitz, K., Helfand, S. L., Tatarb, M. and Sinclair, D. A. (2004). Sirtuin activators mimic calorie restriction and delay aging in metazoans. Nature, 430: 686-689 https://doi.org/10.1038/nature02789
  22. Johannes, H., Bauer, S. G., Graham, B. G. and Stephen, L. H. (2004) An accelerated assay for the identification of lifespan-extending interventions in Drosophila melanogaster. PNAS, 101: 12980-12985 https://doi.org/10.1073/pnas.0403493101
  23. Konrad, T. H., Kevin, J. B., Haim, Y. C., Dudley, W. L., Siva, L., Jason, G. W., Robert, E. Z., Phuong, C., Anne, K., Zhang., L. L., Brandy S. and David, A. S. (2003) Small molecule activators of sirtuins extend Saccharomyces cerevisiae life span. Nature, 425: 191-196 https://doi.org/10.1038/nature01960
  24. Weindruch, R. and Walford, R. L. (1982) Dietary restriction in mice beginning at 1 year of age: effect on life-span and spontaneous cancer incidence. Science, 215: 1415-1418 https://doi.org/10.1126/science.7063854
  25. Chen, D., Steele, A. D., Lindquist, S. and Guarente, L. (2005). Increase in activity during calorie restriction requires Sirt1. Science, 310: 1641 https://doi.org/10.1126/science.1118357