Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidant Activity and Cytotoxic Effect of Extracts from Euphorbia humifusa

땅빈대 추출물의 항산화 활성 및 세포독성 효과

  • Heo, Seong-Il (School of Biotechnology, Kangwon National University) ;
  • Hu, Wei-Cheng (School of Biotechnology, Kangwon National University) ;
  • Han, Woong (School of Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • 허성일 (강원대학교 생명공학부) ;
  • 호위성 (강원대학교 생명공학부) ;
  • 한웅 (강원대학교 생명공학부) ;
  • 왕명현 (강원대학교 생명공학부)
  • Published : 2008.12.31
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Abstract

The antioxidant activities, anti-inflammatory activity and cytotoxic effects of methanol extract from Euphorbia humifusa were evaluated in this study. Total phenolic compound contents were $68.35{\pm}0.16$ mg/g and total flavonoid compound contents were estimated as $38.74{\pm}1.26$ mg/g. EC50 values for DPPH radical scavenging activity of methanol extract was $56.26{\pm}0.66{\mu}g/mL$ and those of positive controls as ascorbic acid, ${\alpha}$-tocopherol and BHA were $8.38{\pm}0.14{\mu}g/mL$, $16.45{\pm}0.89{\mu}g/mL$ and $21.18{\pm}1.01{\mu}g/mL$ respectively. NO scavenging activity increased in depending on concentration of extract. Treatment of RAW 264.7 cells with extract caused inhibition of LPS-induced nitric oxide production. The cell viability showed that the methanol extract had cytotoxicity in the growth of breast cancer cell ($66.54{\pm}1.91%$ at $400{\mu}g/mL$ conc., $43.98{\pm}3.35%$ at $800{\mu}g/mL$ conc.). Based on the results, It was suggested that the methanol extract of Euphorbia humifusa has a potential candidate for functional cosmetic and medicine.

Keywords

References

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