Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anti-oxidative Effect of Some Plant Extracts Against Nitric Oxide-induced Oxidative Stress on Neuronal Cell

Nitric oxide에 의해 산화적 스트레스를 받은 Neuronal cell에 항산화 효과를 가지는 수종 생약추출물의 검색

  • Koo, Uk (Natural Product Research Institute, College of Pharmacy, Seoul National University) ;
  • Lee, Hak-Ju (Korea Forest Research Institute) ;
  • Lee, Dong-Ho (College of Life sciences & Biotechnology, Korea University) ;
  • Lee, Hyun-Jung (Korea Forest Research Institute) ;
  • Ham, Ah-Rom (Natural Product Research Institute, College of Pharmacy, Seoul National University) ;
  • Cho, Eun-Young (Natural Product Research Institute, College of Pharmacy, Seoul National University) ;
  • Mar, Woong-Chon (Natural Product Research Institute, College of Pharmacy, Seoul National University)
  • 구억 (서울대학교 약학대학 천연물과학 연구소) ;
  • 이학주 (국립산림과학원) ;
  • 이동호 (고려대학교 생명과학대학) ;
  • 이현정 (국립산림과학원) ;
  • 함아롬 (서울대학교 약학대학 천연물과학 연구소) ;
  • 조은영 (서울대학교 약학대학 천연물과학 연구소) ;
  • 마응천 (서울대학교 약학대학 천연물과학 연구소)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide ($O_2^-$), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides (炒)(barks), Paeonia japonica (曝乾)(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia (去心, 炒)(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas (當歸尾)(roots) showed anti-oxidative effects[$EC_{50}$<15.20 ${\mu}g$/ml(Carnosine:Positive control)]in dose dependent manner.

Keywords

References

  1. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39: 44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  2. Solov'eva, E. I., Mironova, O. P., Baranova, O. A., Bekman, E. M., Asechev, A. V., Fedin, A. I. and Azizova, O. A. (2008) The free-radical processes and antioxidant therapy in brain ischemia. Zh. Nevrol. Psikhiatr. Im. S S Korsakova. 108: 37-42
  3. Castillo, J., Rama, R. and Davalos, A. (2000) Nitric oxiderelated brain damage in acute ischemic stroke. Stroke 31: 852-857 https://doi.org/10.1161/01.STR.31.4.852
  4. Reeve, A. K., Krishnan, K. J. and Turnbull, D. M. (2008) Age related mitochondrial degenerative disorders in humans. Biotechnol. J. 3: 750-756 https://doi.org/10.1002/biot.200800066
  5. Puddu, P., Puddu, G. M., Cravero, E., Rosati, M. and Muscari, A. (2008) The molecular sources of reactive oxygen species in hypertension. Blood Press 17: 70-77 https://doi.org/10.1080/08037050802029954
  6. Knott, A. B., Perkins, G., Schwarzenbacher, R. and Bossy-Wetzel, E. (2008) Mitochondrial fragmentation in neurodegeneration. Nat. Rev. Neurosci. 9: 505-518 https://doi.org/10.1038/nrn2417
  7. Calabrese, V., Colombrita, C., Guagliano, E., Sapienza, M., Ravagna, A., Cardile, V., Scapagnini, G., Santoro, A. M., Mangiameli, A., Butterfield, D. A., Giuffrida Stella, A. M. and Rizzarelli, E. (2005) Protective effect of carnosine during nitrosative stress in astroglial cell cultures. Neurochem. Res. 30: 797-807 https://doi.org/10.1007/s11064-005-6874-8
  8. Chasovnikova, L. V., Formazyuk, V. E., Sergienko, V. I., Boldyrev, A. A. and Severin, S. E. (1990) The antioxidative properties of carnosine and other drugs. Biochem. Int. 20: 1097-1103
  9. Boldyrev, A. A., Dupin, A.M., Bunin, A. Y., Babizhaev, M. A. and Severin, S. E. (1987) The antioxidative properties of carnosine, a natural histidine containing dipeptide. Biochem. Int. 15: 1105-1113
  10. Veretianov II. (1956) Treatment of hypertension with tincture of Cortex Eucommiae. Voen. Med. Zh. 12: 57-58
  11. Chang, G. T., Min, S. Y., Kim, J. H., Kim, S. H., Kim, J. K. and Kim, C. H. (2005) Anti-thrombic activity of Korean herbal medicine, Dae-Jo-Whan and its herbs. Vascul. Pharmacol. 43: 283-288 https://doi.org/10.1016/j.vph.2005.08.014
  12. Mohammad, Y. M., Divani, A. A., Kirmani, J. F., Harris-Lane, P., Qureshi, A. I. (2004) Acute treatment for ischemic stroke in 2004. Emerg Radiol. 11: 83-86 https://doi.org/10.1007/s10140-004-0363-2