Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Evaluation for Serological Patterns and Fecal Viral Shedding by Hemagglutination Inhibition Test and Real-time PCR in Korean CPV-2 isolates

  • Moon, Hyeong-Sun (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Joon-Seok (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University) ;
  • Nam, So-Jeong (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University) ;
  • Yoon, Soon-Seek (National Veterinary Research & Quarantine Service) ;
  • Kang, Moon-Il (National Veterinary Research & Quarantine Service) ;
  • Jeoung, Seok-Yong (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Doo (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University) ;
  • Hyun, Chang-Baig (Section of Veterinary Internal Medicine, School of Veterinary Medicine, Kangwon National University)
  • Published : 2008.12.31
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Abstract

We evaluated the patterns of serology and fecal viral shedding for any differences by hemagglutination inhibition (HI) and real-time PCR on Korean CPV-2 isolates (CPV-2a-I, CPV-2a-V and CPV-2b). We successfully detected fecal viral shedding from samples extracted 2-3 d.p.i., regardless of the onset of clinical signs. In addition, the pattern of viral shedding differed depending on the CPV-2 isolates used for inoculation. We also observed differences in the serological pattern that was also depended on the CPV-2 isolates inoculated. The onset and amount of fecal viral shedding were not correlated with the level of antibody titers in this study. Our study is a valuable resource for understanding the different pathobiology of the CPV-2 isolates and the correlation between the patterns of serum antibody titer and fecal viral shedding.

Keywords

References

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