Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

The Experimental study of Hwagae-san on Anti-Inflammatory Effect

화개산(華蓋散)의 항염에 대한 실험적 연구

  • No, Woon-Serb (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Jo-Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Si-Hyeong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 노운섭 (원광대학교 한의과대학 폐계내과학교실) ;
  • 신조영 (원광대학교 한의과대학 폐계내과학교실) ;
  • 이시형 (원광대학교 한의과대학 폐계내과학교실)
  • Published : 2008.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : The purpose of this study was to investigate the anti-inflammatory effects of Hwagae-san extract(HGSE) on the peritoneal macrophage. Methods : To evaluate anti-inflammatory effects of HGSE, We measured cytokines(interleukin-6; IL-6, interleukin-12; IL-12, tumor necrosis factor-${\alpha}$; TNF-${\alpha}$) and nitric oxide(NO) production in lipopolysaccharide(LPS)-induced macrophages. Furthermore, We examined molecular mechanism using western blot and also LPS-induced endotoxin shock. Results : 1. HGSE did not have any cytotoxic effect in the peritoneal macrophages. 2. HGSE reduced LPS-induced IL-6, TNF-${\alpha}$, IL-12 and NO production in peritoneal macrophages. 3. HGSE inhibited the activation of extracelluar signal-regulated kinase(ERK), C-Jun NH2-terminal kinase(JNK) but not of p38, degradation of IkB-${\alpha}$ in the LPS-stimulated peritoneal macrophages. 4. HGSE inhibited the production of TNF-$\alpha$, IL-6 and IL-12 in serum after LPS injection. Conclusion : These results suggest that HGSE may inhibit the production of TNF-${\alpha}$, IL-6, and IL-12 through inhibition of ERK and JNK activation, and that HGSE may be beneficial for inflammatory diseases.

Keywords