The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
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The micro-tensile bond strength of two-step self-etch adhesive to ground enamel with and without prior acid-etching

산부식 전처리에 따른 2단계 자가부식 접착제의 연마 법랑질에 대한 미세인장결합강도

  • Kim, You-Lee (Dept. of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Kim, Jee-Hwan (Dept. of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Shim, June-Sung (Dept. of Prosthodontics, College of Dentistry, Yonsei University) ;
  • Kim, Kwang-Mahn (Dept. and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University) ;
  • Lee, Keun-Woo (Dept. of Prosthodontics, College of Dentistry, Yonsei University)
  • 김유리 (연세대학교 치과대학 보철과학교실) ;
  • 김지환 (연세대학교 치과대학 보철과학교실) ;
  • 심준성 (연세대학교 치과대학 보철과학교실) ;
  • 김광만 (연세대학교 치과대학 치과생체재료공학교실) ;
  • 이근우 (연세대학교 치과대학 보철과학교실)
  • Published : 2008.04.30
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Abstract

Statement of problems: Self-etch adhesives exhibit some clinical benefits such as ease of manipulation and reduced technique-sensitivity. Nevertheless, some concern remains regarding the bonding effectiveness of self-etch adhesives to enamel, in particular when so-called 'mild' self-etch adhesives are employed. This study compared the microtensile bond strengths to ground enamel of the two-step self-etch adhesive Clearfil SE Bond (Kuraray) to the three-step etch-and- rinse adhesive Scotchbond Multi-Purpose (3M ESPE) and the one-step self-etch adhesive iBond (Heraeus Kulzer). Purpose: The purpose of this study was to determine the effect of a preceding phosphoric acid conditioning step on the bonding effectiveness of a two-step self-etch adhesive to ground enamel. Material and methods: The two-step self-etch adhesive Clearfil SE Bond non-etch group, Clearfil SE Bond etch group with prior 35% phosphoric acid etching, and the one-step self-etch adhesive iBond group were used as experimental groups. The three-step etch-and-rinse adhesive Scotchbond Multi-Purpose was used as a control group. The facial surfaces of bovine incisors were divided in four equal parts cruciformly, and randomly distributed into each group. The facial surface of each incisor was ground with 800-grit silicon carbide paper. Each adhesive group was applied according to the manufacturer's instructions to ground enamel, after which the surface was built up using Light-Core (Bisco). After storage in distilled water at $37^{\circ}C$ for 1 week, the restored teeth were sectioned into enamel beams approximately 0.8*0.8mm in cross section using a low speed precision diamond saw (TOPMET Metsaw-LS). After storage in distilled water at $37^{\circ}C$ for 1 month, 3 months, microtensile bond strength evaluations were performed using microspecimens. The microtensile bond strength (MPa) was derived by dividing the imposed force (N) at time of fracture by the bond area ($mm^2$). The mode of failure at the interface was determined with a microscope (Microscope-B nocular, Nikon). The data of microtensile bond strength were statistically analyzed using a one-way ANOVA, followed by Least Significant Difference Post Hoc Test at a significance level of 5%. Results: The mean microtensile bond strength after 1 month of storage showed no statistically significant difference between all adhesive groups (P>0.05). After 3 months of storage, adhesion to ground enamel of iBond was not significantly different from Clearfil SE Bond etch (P>>0.05), while Clearfil SE Bond non-etch and Scotchbond Multi-Purpose demonstrated significantly lower bond strengths (P<0.05), with no significant differences between the two adhesives. Conclusion: In this study the microtensile bond strength to ground enamel of two-step self-etch adhesive Clearfil SE Bond was not significantly different from three-step etch-and-rinse adhesive Scotchbond Multi-Purpose, and prior etching with 35% phosphoric acid significantly increased the bonding effectiveness of Clearfil SE Bond to enamel at 3 months.

자가부식 접착제는 사용하기 쉽고, 술식 민감성이 적은 장점이 있으나 특히 산도가 약한 자가부식 접착제의 법랑질에 대한 결합력은 논란이 되고 있다. 본 연구에서는 2단계 자가부식 접착제인 Clearfil SE Bond (Kuraray, Okayama, Japan)의 연마 법랑질에 대한 미세인장 결합강도를 측정하여 3단계 산부식수세 접착제인 Scotchbond Multi-Purpose (3M ESPE, St. Paul, MN, USA) 및 1단계 자가부식 접착제인 iBond (Heraeus Kulzer Gmbh, Hanau, Germany)의 결합강도와 비교하고자 하였고, 2단계 자가부식 접착제에 산부식 전처리를 시행하는 것이 법랑질에 대한 결합강도를 높일 수 있는지 알아 보고자 하였다. 실험군은 2단계 자가부식 접착제인 Clearfil SE Bond만 사용한 비산부식 군과 35% 인산 (Scotchbond Etchant, 3M ESPE)으로 산부식 후 Clearfil SE Bond를 사용한 산부식 군, 그리고 1단계 자가부식 접착제인 iBond를 사용한 군으로 나누었다. 대조군은 3단계 산부식수세 접착제인 Scotchbond Multi-Purpose를 사용한 군으로 정하였다. Bovine 전치의 순면을 십자가형으로 4등분하여 각 군으로 무작위로 배분하였다. 각 치아의 순면을 800-grit 실리콘 카바이드 지로 연마한 후 삭제된 법랑질면에 제조사의 설명서에 따라 각 군의 접착제를 적용하고 Light-Core (Bisco)로 적층 충전하였다. 시편은 $37i{\acute{E}}$, 증류수에 일주일 동안 보관한 후 low speed precicion diamond saw (TOPMENT Metsaw-LS, R&B, Daejeon, Korea)를 이용하여 약 $0.8{\times}0.8mm$ 단면이 되도록 시편을 절단하여 미세시편을 제작하였다. 일주일마다 증류수를 교환하면서 한 달, 세 달 동안 $37i{\acute{E}}$, 증류수에 미세시 편을 보관한 후 각각의 미세인장결합강도를 측정하였다. 미세인장결합강도 (MPa)는 파절 시에 가해진 힘 (N)을 접착면적 ($mm^2$)으로 나누어 계산하였다. 접착계면에서의 파절 양상은 실물현미경 (Microscope-B nocular, Nikon)을 이용하여 분류하였다. 미세인장결합강도에 대한 통계분석은 one-way ANOVA를 이용하여 유의수준 5%에서 검정하였고, 사후감정은 Least Significant Difference 방법을 이용하였다. 중합 후 1개월 뒤 측정된 각각의 접착제의 평균 미세인장결합강도는 유의수준 5%에서 모든 접착제 간에 유의한 차이가 없었다. 3개월 뒤 측정된 각각의 접착제의 평균 미세인장결합강도는 유의수준 5%에서 iBond 와 Clearfil SE Bond 비산부식 군과 Scotchbond Multi- Purpose 간에는 각각 유의한 차이가 없었다. 본 연구에서는 2단계 자가부식 접착제인 Clearfil SE Bond의 연마 법랑질에 대한 미세인장결합강도가 3단계 산부식수세 접착제인 Scotchbond Multi-Purpose 와 비교하여 유의한 차이가 없었으며 (P>0.05), 3개월 후의 결과에서 Clearfil SE Bond 비산부식 군의 미세인장결합강도가 Clearfil SE Bond 산부식 군보다 유의하게 낮았다 (P<0.05). 즉 35% 인산으로 산부식 전처리를 시행한결과 Clearfil SE Bond 의 법랑질에 대한 결합강도가 증가하였다.

Keywords

References

  1. Van Meerbeek B, Vargas M, Inoue S, Yoshida Y, Peumans M, Lambrechts P, Vanherle G. Adhesives and cements to promote preservation dentistry. Oper Dent 2001;26:119-44
  2. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G. Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent 2003;28:215-35
  3. De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, Van Meerbeek B. A critical review of the durability of adhesion to tooth tissue : methods and results. J Dent Res 2005;84:118-32 https://doi.org/10.1177/154405910508400204
  4. Frankenberger R, Tay FR. Self-etch vs etch-and-rinse adhesives : effect of thermo-mechanical fatigue loading on marginal quality of bonded resin composite restorations. Dent Mater 2005;21:397-412 https://doi.org/10.1016/j.dental.2004.07.005
  5. Park YJ. Current adhesive dentistry for dental restoratives. J Korean Dental Association 2003;41:478-91
  6. Leem SH, Lee JH. Shear bond strength of self-etching adhesives to enamel. J Korean Dental Association 2004;42 :790-7
  7. Peumans M, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B. Clinical effectiveness of comtemporary adhesives : A systematic review of current clinical trials. Dent Mater 2005;21:864-81 https://doi.org/10.1016/j.dental.2005.02.003
  8. Yoshida Y, Nagakane K, Fukuda R, Nakayami Y, Okazaki M, Shintani H. Comparative study on adhesive performance of functional monomers. J Dent Res 2004;83:454-8 https://doi.org/10.1177/154405910408300604
  9. Miguez PA, Castro PS, Nunes MF, Walter R, Pereira PNR. Effect of acid-etching on the enamel bond of two self-etching systems. J Adhes Dent 2003;5:107-12
  10. Armstrong SR, Vargas MA, Fang Q, Laffoon JE. Microtensile bond strength of a total-etch 3-step, total-etch 2-step, self-etch 2-step, and a self-etch 1-step dentin bonding system through 15-month water storage. J Adhes Dent 2003;5:47-56
  11. Perdigao J, Geraldeli S. Bonding characteristics of selfetching adhesives to intact vs. prepared enamel. J Esthet Restor Dent 2003;15:32-42 https://doi.org/10.1111/j.1708-8240.2003.tb00280.x
  12. Inoue S, Vargas MA, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H, Van Meerbeek B. Microtensile bond strength of eleven contemporary adhesives to dentin. J Adhes Dent 2001;3:237-45
  13. Van Landuyt KL, Kanumilli P, De Munck J, Peumans M, Lambrechts P, Van Meerbeek B. Bond strength of a mild self-etch adhesive with and without prior acid-etching. J Dent 2006;34:77-85 https://doi.org/10.1016/j.jdent.2005.04.001
  14. Van Meerbeek B, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Peumans M. A randomized controlled study evaluating the effectiveness of a two-step self-etch adhesive with and without selective phosphoric-acid etching of enamel. Dent Mater 2005;21:375-83 https://doi.org/10.1016/j.dental.2004.05.008
  15. Nakamichi I, Iwaku M, Fusayama T. Bovine teeth as possible substitutes in the adhesion test. J Dent Res 1983; 62:1076-81 https://doi.org/10.1177/00220345830620101501
  16. Miyazaki M, Hirohata N, Takagaki K, Onose H, Moore BK. Influence of self-etching primer drying time on enamel bond strength of resin composites. J Dent 1999;27:203-7 https://doi.org/10.1016/S0300-5712(98)00044-X
  17. Reeves GW, Fitchie JG, Hembree JH, Puckett AD. Microleakage of new dentin bonding systems using human and bovine teeth. Oper Dent 1995;20:230-5
  18. Reis AF, Giannini M, Kavaguchi A, Soares CJ, Line SRP. Comparison of microtensile bond strength to enamel and dentin of human, bovine, and porcine teeth. J Adhes Dent 2004;6:117-21
  19. Brackett WW, Ito S, Nishitani Y, Haisch LD, Pashley DH. The microtensile bond strength of self-etching adhesives to ground enamel. Oper Dent 2006;31:332-7 https://doi.org/10.2341/05-38
  20. Torii Y, Itou K, Nishitani Y, Ishikawa K, Suzuki K. Effect of phosphoric acid etching prior to self-etching primer application on adhesion of resin composite to enamel and dentin. Am J Dent 2002;15:305-8
  21. Turkun SL. Clinical evaluation of a self-etching and a onebottle adhesive system at two-years. J Dent 2003;31:527-34 https://doi.org/10.1016/S0300-5712(03)00107-6
  22. Peumans M, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B. Three-year clinical effectiveness of a two-step self-etch adhesive in cervical lesions. Eur J Oral Sci 2005;113:512-8 https://doi.org/10.1111/j.1600-0722.2005.00256.x