Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Binding Affinities of Carbohydrate-Conjugated Chlorins for Galectin-3

  • Park, Young K. (PDT Research Institute, Department of Nano System Engineering, Inje University) ;
  • Bold, Bayarmaa (PDT Research Institute, Department of Nano System Engineering, Inje University) ;
  • Cui, Bing Cun (PDT Research Institute, Department of Nano System Engineering, Inje University) ;
  • Bai, Jin Quan (PDT Research Institute, Department of Nano System Engineering, Inje University) ;
  • Lee, Woo-Kyoung (PDT Research Institute, Department of Nano System Engineering, Inje University) ;
  • Shim, Young-Key (PDT Research Institute, Department of Nano System Engineering, Inje University)
  • Published : 2008.01.20
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Abstract

Carbohydrate-conjugated chlorins were synthesized for use as biosensors for the detection of the galectin-3 cancer marker. We used ELISA, SDS-gel electrophoresis, and Bradford assays to examine the binding of galectins to d-(+)-galactose- and b-lactose-conjugated chlorins. The binding affinities of these conjugated chlorins for galectin-3 were quantified using fluorescence spectroscopy. The fluorescence emission of the carbohydrate-conjugated chlorins decreased as the amount of galectin-3 in the binding reaction increased over a limited concentration range, indicating that carbohydrate-conjugated chlorins are potentially useful fluorescence biosensors for the galectin-3 cancer marker.

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