Journal of Microbiology and Biotechnology

  • 제18권10호
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  • Pages.1735-1740
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Isolation and Characterization of PERV-C env from Domestic Pig in Korea

  • Park, Sung-Han (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Bae, Eun-Hye (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Park, Sang-Min (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Park, Jin-Woo (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Lim, Mi-Suk (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Jung, Yong-Tae (Department of Microbiology and Institute of Basic Science, Dankook University)
  • 발행 : 2008.10.31
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초록

Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.

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