Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Lectin Histochemistry on the Effects of Smoking on Glycoconjugates of Rat Sebaceous Glands

흡연이 흰쥐 복부 피지샘의 glycoconjugates에 미치는 영향에 대한 lectin 조직화학

  • Jo, Un-Bock (Biology Education Major, Division of Science Education, College of Education, Pusan National University) ;
  • Jeong, Gil-Nam (Biology Education Major, Division of Science Education, College of Education, Pusan National University)
  • 조운복 (부산대학교 사범대학 과학교육학부 생물교육) ;
  • 정길남 (부산대학교 사범대학 과학교육학부 생물교육)
  • Published : 2008.11.30
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Abstract

We studied the effects of smoking, which is one of indoor-environmental pollutants and related to various cancers, on glycoconjugates of rat sebaceous glands with the lectin histochemistry. To investigate the effects of smoking on glycoconjugates, male Sprague-Dawley rats were exposed to tabacco smoke for 10 minutes per day in an inhalation chamber for 1, 2, 3, and 5 days with active and passive exposure. For the structure of sebaceous glands we used PAS reaction, and for the glycoconjugates binding pattern 9 biotinylated lectins (DBA, SBA, PNA, BSL-1, WGA, RCA-1, UEA-1, Con A, and LCA) were used. Some remarkable changes, such as the decrease in the size of sebaceous glandular acini, the destruction of upper portion of sebaceous glands, vacuolation of central portion of sebocytes, and the immature sebaceous glandular acini were seen in the smoke-exposed rats. In the control rats, basal cells were stained with BSL-1, PNA and WGA, but the stronger reaction was founded in BSL-1 binding. Also, sebocytes were stained with PNA, WGA, Con A, BSL-1 and SBA, but stronger reactions were founded in PNA and Con A stainings. Specific changes in the lectin binding patterns were also observed in the smoke-exposed rats. In the basal cells of exposed rats, PNA binding increased, BSL-1 decreased but returned to control level, and WGA disappeared. Plus, immature glandular acini, which were not found in the control rats, were stained PNA, Con A and BSL-1, but the stronger reaction were founded in PNA and Con A binding. In conclusion, it was assumed that the tabacco smoke seriously effected on the structure and glycoconjugates metabolism of sebaceous glands.

실내 환경 오염원으로 각종 암 발병과 관련이 있는 흡연이 피부 피지샘의 glycoconjugates에 미치는 영향을 lectin 조직화학으로 연구하였다. Sprague-Dawley계 수컷 흰쥐에 1일 1회 10분간 1일군, 2일군, 3일군 및 5일군으로 나누어 직?간접 흡연 방법으로 폭로시켜 피지샘 형태는 PAS 염색, 피지샘의 glycoconjugates 양상을 9종의 biotinylated lectin (DBA, SBA, PNA, BSL-1, WGA, RCA-1, UEA-1, Con A 및 LCA)으로 연구하였다. 대조군에 비해 흡연군에서는 피지샘꽈리의 크기 감소, 피지샘꽈리 상부의 파괴, 중앙부 피지세포의 공포화 및 미성숙 피지샘꽈리가 관찰되었다. 대조군에서 피지샘 glycoconjugates에는 염색성에 차이가 있으나 기저세포는 BSL-1, PNA 및 WGA에만 반응하고 BSL-1의 반응이 더 강하며, 피지세포는 PNA, WGA, Con A, BSL-1 및 SBA에만 반응하고 PNA 및 Con A의 반응이 더 강했다. 흡연군의 렉틴 반응에서 특이적인 변화가 관찰되었다. 흡연군의 피지샘 기저세포의 PNA 반응은 증가되었고, BSL-1는 감소되었다가 회복되었으며, WGA는 소실되었다. 피지세포의 Con A는 증가하였으나 PNA, BSL-1, WGA 및 SBA는 감소 또는 소실되었다. 대조군에 관찰되지 않던 흡연군의 미성숙 샘꽈리 PNA, on A 및 BSL-1이 반응하였으며 PNA 및 Con A의 반응이 더 현저하였다. 결론적으로 흡연은 피지샘의 형태 및 glycoconjugates 대사에 심한 영향을 미치는 것으로 사료된다.

Keywords

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