Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cytotoxic Effect of Isolated Protein-bound Polysaccharides from Hypsizigus marmoreus Extracts by Response Surface Methodology

반응표면분석에 의한 해송이버섯(Hypsizigus marmoreus) 추출물 중 단백다당체의 암세포 성장억제효과

  • Published : 2008.12.31
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Abstract

This study used response surface methodology (RSM) in an effort to optimize the water extraction conditions of Hypsizigus marmoreus in order to increase cytotoxicity activity of the extract. A central composite design was applied to investigate the effects of independent variables, which included the extraction temperature ($X_1$), extraction time ($X_2$), the ratio of solvent to sample ($X_3$) on dependent variables of the extracts, including extraction yield ($Y_1$) and protein content ($Y_2$). The estimated optimal conditions were as follows: $51.3^{\circ}C$ extraction temperature, 8.2 hrs extraction time, and 46.7 mL/g of solvent per sample. The extract (CE) was extracted at optimal condition and crude polysaccharides (CPS) were obtained from CE by ethanol precipitation, dialysis, and freeze drying. Neutral (NPS) and acidic (APS) fraction of polysaccharides were seperated from CPS by ion chromatography. The growth inhibitory effects of the APS (0.5 mg/mL) on AGS human cancer cells were 73.97%. CPS showed the highest growth inhibitory effects on HepG2 human cancer cell at 0.5 mg/mL. However all fraction polysaccharides from Hypsizigus marmoreus showed lower than 20% growth inhibition on SW480 human cancer cell.

해송이버섯 분말의 암세포성장억제효과의 증진을 위한 성분의 추출조건을 최적화하기 위하여 반응표면분석법을 사용하였다. 중심합성계획에 따라 추출조건의 독립변수(추출온도, 추출시간, 용매비)와 이에 따라 영향을 받는 수율과 추출물의 단백질 함량을 종속변수로 설정하였다. 수율과 단백질 함량이 높은 조건을 충족시키는 최적조건은 추출온도 $51.3^{\circ}C$, 추출시간 8.2시간, 그리고 추출용매비는 46.7 mL/g 으로 나타났다. 최적조건에서 추출되어진 조추출물을 이용하여 알코올을 이용하여 침전한 후 dialysis tube를 이용하여 저분자물질을 제거하여 조다당체를 얻었으며 조다당체를 이용하여 이온교환수지를 이용하여 산성다당체와 중성다당 체를 얻어 각각의 다당체의 암세포성장억제효과를 알아보았다. 위암세포주 AGS에는 산성다당체가 0.5 mg/mL의 농도에서 73.97%로 가장 높은 세포증식억제효과를 보였으며 간암세포주 HepG2에는 조다당체가 0.5 mg/mL의 농도에서 82.45%로 가장 높은 효과를 보였다. 그러나 결장암세포주 SW480에서는 모든 분획의 시료가 20%미만의 세포성장 억제효과를 보였다.

Keywords

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