DOI QR코드

DOI QR Code

Effects of Diluents and Cryoprotectants on Sperm Cryopreservation of Mason Salmon, Oncorhynchus masou masou

산천어(Oncorhynchus masou masou) 정자의 냉동보존에 미치는 희석액과 동해방지제의 영향

  • Lim, Han-Kyu (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Lee, Cheul-Ho (Cold-Water Inland Fisheries Research Institute) ;
  • Min, Byung-Hwa (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Lee, Jung-Uie (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Lee, Chae-Sung (Cold-Water Inland Fisheries Research Institute) ;
  • Seong, Ki-Baik (Cold-Water Inland Fisheries Research Institute) ;
  • Lee, Sang-Mok (Aquaculture Management Division, National Fisheries Research and Development Institute)
  • 임한규 (국립수산과학원 양식관리과) ;
  • 이철호 (국립수산과학원 영동내수면연구소) ;
  • 민병화 (국립수산과학원 양식관리과) ;
  • 이정의 (국립수산과학원 양식관리과) ;
  • 이채성 (국립수산과학원 영동내수면연구소) ;
  • 성기백 (국립수산과학원 영동내수면연구소) ;
  • 이상목 (국립수산과학원 양식관리과)
  • Published : 2008.08.31

Abstract

We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained $18{\pm}1mmol/L$ potassium, $144{\pm}4mmol/L$ sodium, $116{\pm}3mmol/L$ chloride, $83.2{\pm}3.1mg/L$ calcium, $14.8{\pm}0.7mg/L$ magnesium, $45{\pm}9mg/L$ glucose, and $1.0{\pm}0.0g/L$ total protein. The osmolality and pH of seminal plasma were $287{\pm}7\;and\;7.7{\pm}0.1mmol/kg$, respectively, and the spermatocrit was $28{\pm}2$. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300 mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.

Keywords

References

  1. Billard, R., J. Cosson, S.B. Noveiri and M. Pourkazemi. 2004. Cryopreservation and short-term storage of sturgeon sperm, a review. Aquaculture, 236, 1-9 https://doi.org/10.1016/j.aquaculture.2003.10.029
  2. Blaxter, J.H.S. 1953. Sperm storage and cross fertilization of spring and autumn spawning herring. Nature, 172, 1189-1190
  3. Cabrita, E., L. Anel and M.P. Herraéz. 2001a. Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm. Theriogenology, 56, 623-635 https://doi.org/10.1016/S0093-691X(01)00594-5
  4. Cabrita, E., V. Robles, R. Alvarez and M.P. Herraez. 2001b. Cryopreservation of rainbow trout sperm in large volume straws: application to large scale fertilization. Aquaculture, 201, 301-314 https://doi.org/10.1016/S0044-8486(01)00636-6
  5. Chang, Y.J. and Y.J. Chang. 2002. Milt properties of four flatfish species and fine structure of their cryo- preserved spermatozoa. J. Fish. Sci. Tech., 5, 87-96 https://doi.org/10.5657/fas.2002.5.2.087
  6. Chao, N.H. and I.C. Liao. 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture, 197, 161-189 https://doi.org/10.1016/S0044-8486(01)00586-5
  7. Ciereszko, A. and K. Dabrowski. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture, 109, 367-373 https://doi.org/10.1016/0044-8486(93)90175-X
  8. Ciereszko, A. and K. Dabrowski. 1996. Effect of a sucrose-DMSO extender supplemented with pen- toxifylline or blood plasma on fertilizing ability of cryopreserved rainbow trout spermatozoa. Prog. Fish-Cult., 58, 143-145 https://doi.org/10.1577/1548-8640(1996)058<0143:EOASDE>2.3.CO;2
  9. Erdhal, D. and E.F. Graham. 1980. Cryopreservation of spermatozoa of the brook and rainbow trout. CryoLett., 1, 203-208
  10. Gwo, J.C. 1994. Cryopreservation of yellowfin seabream (Acanthopagrus latus) spermatozoa. Theriogenology, 41, 989-1004 https://doi.org/10.1016/S0093-691X(05)80022-6
  11. Jamieson, B.G.M. 1991. Fish Evolution and Systematics: Evidence from Spermatozoa. Cambridge University Press, New York, USA, 319
  12. Kurokura, H. and R. Hirano. 1980. Cryopreservation of rainbow trout sperm. Bull. Jap. Soc. Sci. Fish., 46, 1493-1495 https://doi.org/10.2331/suisan.46.1493
  13. Kusuda, S. 2004. Current status and perspective of cryopreservation of sperm and blastomeres in fish. Fish Genet. Breed. Sci., 34, 1-25
  14. Lahnsteiner, F., N. Mansour and T. Weismann. 2002. A new technique for insemination of large egg batches with cryopresered semen in the rainbow trout. Aquaculture, 209, 359-367 https://doi.org/10.1016/S0044-8486(01)00869-9
  15. Lahnsteiner, F., T. Weismann and R.A. Patzner. 1997. Methanol as cryoprotectant and the suitability of 1.2 mL and 5mL straws for cryopreservation of semen from salmonid fishes. Aquacult. Res., 28, 471-479 https://doi.org/10.1111/j.1365-2109.1997.tb01065.x
  16. Le, M.H., H.K. Lim, B.H. Min, S.Y. Kim and Y.J. Chang. 2007. Milt properties and spermatozoa structure of filefish (Thamnaconus modestus). Dev. Reprod., 11, 227-233
  17. Lim, H.K. 1998. Physiological properties of sperm and gamete preservation in black seabream, Acantho- pagrus schlegeli. Ph.D. Thesis, Pukyong National University, Korea, 1-130
  18. Lim, H.K., C.M. An, M.H. Son, M.W. Park, E.O. Kim and S.G. Byun. 2006. Effects of diluents and temperature on sperm storage in starry flounder (Platichthys stellatus). Kor. J. Aquacult., 19, 47-51
  19. Lin, F., L. Liu and K. Dabrowski. 1996. Characteristics of muskellunge spermatozoa. I: Ultrastructure of spermatozoa and biochemical composition of semen. Trans. Am. Fish. Soc., 125, 187-194 https://doi.org/10.1577/1548-8659(1996)125<0187:COMSIU>2.3.CO;2
  20. Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleosts. Zool. Sci., 2, 605-615
  21. Ohta, H., H. Shimma and K. Hirose. 1995. Effects of freezing rate and lowest cooling pre-storage tem- perature on post-thaw fertility of amago and masu salmon spermatozoa. Fish. Sci., 61, 423-427 https://doi.org/10.2331/fishsci.61.423
  22. Yamano, K., N. Kasahara, E. Yamaha and F. Yamazaki. 1990. Cryopreservation of masu salmon sperm by the pellet method. Bull. Fac. Fish., Hokkaido Univ., 41, 149-154

Cited by

  1. Cryopreservation of Semen in Dead Yellow Croaker, Larimichthys polyactis vol.13, pp.4, 2010, https://doi.org/10.5657/fas.2010.13.4.350