Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Biochemical Properties of Hemolysin Produced by Vibrio cholerae non-O1 CT Isolated from Sea Water

해수에서 분리한 Vibrio cholerae non-O1 CT가 생산하는 용혈독소의 생화학적인 특징

  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong University) ;
  • Lee, Myung-Suk (Department of Microbiology, Pukyong University) ;
  • Kim, Young-Man (Department of Food Science and Nutrition, Dongeui University) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dongeui University) ;
  • Yeom, Jong-Hwa (Department of Clinical Laboratory Science, Dongeui University) ;
  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University)
  • Published : 2008.08.31
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Abstract

The hemolysin of Vibrio cholerae non-O1 CT isolated from sea water was purified and characterized. The purified hemolysin displayed an optimum at $37^{\circ}C$ and exhibited more than 70% of residual hemolytic activity after incubation at $45^{\circ}C$ for 120 min. However, the activity dropped dramatically at temperature above $55^{\circ}C$. The purified protein showed the highest hemolytic activity at pH 7.0, while the activity was completely lost outside of the pH ranges of 5.0 and 10.0. The activity of hemolysin was inactivated by addition of divalent cations, such as $Cu^{2+},\;Fe^{2+},\;Hg^{2+},\;Mn^{2+},\;and\;Zn^{2+}$, however, the activity was not completely inhibited by additions of $Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+,\;and\;Li^+$.

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References

  1. Chang, D.S. and S. Shinoda. 1994. Toxin produced by pathogenic Vibrio isolated from sea food. J. Kor. Fish. Soc., 27, 107-113
  2. Chang, D.S., I.S. Shin, S.T. Choi and Y.M. Kim. 1986. Distribution and bacteriological characteristics of Vibrio vulnificus. J. Kor. Fish. Soc., 19, 118-126
  3. Gyobu, Y., H. Kodama and S. Sato. 1992. Studies on the enteropathogenic mechanism of non O1 Vibrio cholerae. IV. Role of hemolysin. Kansenshogaku- Zasshi, 65, 897-904
  4. Han, J.H., J.H. Lee, Y.H. Choi, J.H. Park, T.J. Choi and I.S. Kong. 2002. Purification, characterization and molecular cloning of Vibrio fluvialis hemolysin. Biochim. Biophys. Acta., 1599, 106-114 https://doi.org/10.1016/S1570-9639(02)00407-7
  5. Honda, T., M. Arita, T. Takeda, M. Yoh and T. Miwatani. 1985. Non O1 Vibrio cholerae produces two newly identified of toxins related to Vibrio parahaemolyticus hemolysin and Escherichia coli heat-stable entero- toxin. Lancet, 2, 163-164
  6. Iida, T. and K. Yamamoto. 1990. Cloning and expres- sion of two genes encoding highly homologous hemolysins from a Kanagawa phenomenon-positive Vibrio parahaemolyticus T4750 strain. Gene, 93, 9-15.1 https://doi.org/10.1016/0378-1119(90)90129-F
  7. Ju, J.W. and S.A Ju. 1996. Purification and immunological characterization of $\beta$-hemolysin produced by Vibrio mimicus. J. Bacteriol. Virol., 31, 433-449
  8. Kim, S.H., M.Y. Park, Y.O. Lee, M.H. Cho and D.S. Chang. 1997. Characteristics of hemolysin produced by Vibrio cholerae non-O1 FM-3 Isolated from Sea Water. J. Kor. Fish. Soc., 30, 556-561
  9. Kim, Y.M., I.S. Shin and D.S. Chang. 1987. Distribution of Vibrio vulnificus the coast of South Korea. J. Kor. Fish. Soc., 20, 591-600
  10. Lee, H.K., S.S. Lee, Y.H. Yoon and K.H. Ha. 1998. Biochemical characteristics of Vibrios isolated from cultured shellfish, Ruditapes philippinarum, and some species of wild shellfish. J. Bacteriol. Virol., 33, 567-574
  11. Miyake, M., T. Honda and T. Miwatani. 1989. Effects of divalent cations and saccharides on Vibrio metschnikovii cytolysin-induced hemolysis of rabbit erythrocytes. Infect. Immun., 57, 158-163
  12. Miyoshi, S., K. Sasahara, S. Akamatsu, M.M. Rahman, T. Katsu, K. Tomochika and S. Shinoda. 1997. Purification and characterization of a hemolysin produced by Vibrio mimicus. Infect. Immun., 65, 1830-1835
  13. Oh, E.K., Y. Tamanoi, A. Toyoda, K. Usui, S. Miyoshi, D.S. Chang and S. Shinoda. 1993. Simple purification method for a Vibrio vulnificus hemolysin by a hydrophobic column chromatography in the presence of a detergent. Microbiol. Immunol., 37, 975-978 https://doi.org/10.1111/j.1348-0421.1993.tb01732.x
  14. Park, J.W., T.A. Jahng, H.W. Rho, B.H. Park, N.H. Kim and H.R. Kim. 1994. Inhibitory mechanism of $Ca^{2+}$ on the hemolysis caused by Vibrio vulnificus cytolysin. Biochim. Biophys. Acta., 1194, 166-170 https://doi.org/10.1016/0005-2736(94)90216-X
  15. Seong, H.K. 2003. The Distribution of serogroup of Vibrio cholerae non-O1 isolated in Korea. Kor. J. Microbiol. Biotechnol., 31, 13-17
  16. Shinoda, S., I. Akiyama, S. Yasuil, Y.M. Kim and B.I. Ono. 1992. Lateral flagella of Vibrios serological classification and genetical similarity. Soc. Microbiol. Immunol., 36, 303-307 https://doi.org/10.1111/j.1348-0421.1992.tb01667.x
  17. Shinoda, S., S. Miyoshi, H. Yamanaka and N. Miyoshi- Nakahara. 1985. Some properties of Vibrio vulnificus hemolysin. Microbiol. Immunol., 29, 583-590 https://doi.org/10.1111/j.1348-0421.1985.tb00862.x
  18. Yotaku, G., H. Kodama and S. Sato. 1992. Studies on the enteropathogenic mechanism of non O1 Vibrio cholerae. III. Production of enteroreactive toxins. Kansenshogaku-Zasshi, 65, 781-787
  19. Zitzer, A., I. Walev, M. Palmer and S. Bhakdi. 1995. Characterization of Vibrio cholerae El Tor cytolysin as an oligomerizing pore-forming toxin. Med. Micro- biol. Immunol., 184, 37-44 https://doi.org/10.1007/BF00216788