Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Production of Antifungal Materials by Bacillus sp. Which Inhibit Growth of Phytophthora infestans and Fusarium oxysporum

Phytophthora infestans와 Fusarium oxysporum의 생장을 저해하는 Bacillus 분리균주들의 항진균성 물질 생성능

  • 이강형 (강원대학교 자연과학대학 생명과학부) ;
  • 송홍규 (강원대학교 자연과학대학 생명과학부)
  • Published : 2008.09.30
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Abstract

Late blight, one of the most important disease in many agricultural crops, is caused by Phytophthora infestans. Fusarium wilt is a vascular disease of many plants caused by Fusarium oxysporum. Some bacteria isolated from rhizosphere were screened for their ability to inhibit the growth of F. oxysporum and P. infestans. Productions of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide and chitinase by 4 isolated strains were examined. Among them, Bacillus sp. RFO41 most effectively inhibited the growth of F. oxysporum. The highest productions of siderophore and $\beta-l$,3-glucanase were shown in the culture of Bacillus sp. RFO41. Bacillus strain PS2 was most effective against P. infestans. PS2 showed the highest production of chitinase and hydrogen cyanide. A significant relationship was shown between the antagonistic effects of isolates against F. oxysporum and P. infestans and their production level of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide, and chitinase.

대표적인 식물병원성 곰팡이 인 Phytophthora infestans와 Fusarium oxysporum의 생장을 저해하는 근권세균들을 토양에서 분리하여 동정하였으며 이 균주들이 분비하는 항진균성 물질인 siderophore, $\beta-1$,3-glucanase, hydrogen cyanide와 chitinase의 생성능을 조사하였다. 분리균주 중 Bacilus sp. RFO41은 F. oxysporum의 생장을 가장 효율적으로 억제하였으며, siderophore 생성능과 $\beta-1$,3-glucanase의 활성이 가장 우수하였다. 또 다른 분리균주인 Bacilus sp. PS2는 P. infestans의 생장을 가장 많이 억제하였으며, chitinase 활성과 hydrogen cyanide 생성능이 가장 우수하였다. F. oxysporum과 P. infestans에 대한 항진균 효과는 근권세균이 생산하는 siderophore, $\beta-1$,3-glucanase, hydrogen cyanide와 chitinase의 활성에 따라 차이가 있음을 알 수 있었다.

Keywords

References

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