Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Cyanobacteria Producing Microcystin from Lakes

담수 생태계에서 Microcystin을 생산하는 남조세균의 분리

  • Lee, Hee-Seon (Department of Environmental Engineering, Chungbuk National University) ;
  • Oh, Kyoung-Hee (Biotechnology Research Institute, Chungbuk National University,) ;
  • Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
  • Published : 2008.09.30
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Abstract

Four potential microcystin-producing cyanobacteria were isolated from large reservoirs which act as sources of drinking water supply in Korea. Strain DC-2, YD-l, and YD-6 were closely related to Microcystis aeruginosa based on the analysis of l6S rRNA gene and mcyA gene sequences. mcyA gene sequence of YDS2-3, isolated from Yongdam Reservoir, was closed to that of M. aeruginosa, whereas l6S rRNA gene sequence was not related to the known sequences of microcystin-producing cyanobacteria indicating this strain can be a novel cyanobacterium belonging to the genus Microcystis. When mcyA gene sequences of isolated cyanobacteria were compared with the mcyA gene sequence library of two reservoirs, the sequence of DC-2 matched with the dominant ones.

대청호와 용담호로부터 조류 독소인 microcystin을 생산하는 남조세균을 분리하고, 이의 16S rRNA와 microcystin 생합성 유전자(mcyA)를 분석하였다. 분리된 조류 중 대청호에서 분리된 DC-2와 총담호에서 분리된 YD-1, YD-6은 16S rRNA와 mcyA 유전자 염기서열을분석한 결과 Microcystis aeruginosa에 속하는 것으로 판단되었다. 용담호에서 분리된 YDS2-3의 경우, mcyA 유전자는 M. aeruginosa와 매우 유사하나 16S rRNA 유전자는 매우 상이한 것으로 나타나 Microcystis 속에 속하는 새로운 종일 가능성 이 있는 것으로 판단되었다. 대청호와 용담호 시료로부터 mcyA 유전자 library를 구축하여 분리된 조류의 유전자와 비교한 결과, DC-2의 mcyA 유전자가 두 호수에서 가장 우점하는 것과 일치하였다. 본 연구에서 분리된 조류는 독성 조류의 모니터링 및 제어에 관한 연구에 활용될 수 있을 것으로 사료된다.

Keywords

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