Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Degradation of Bisphenol A and Removal of Its Estrogenic Activity by Two Laccase Transformants of Irpex lacteus

기계충버섯 형질전환체를 이용한 비스페놀 A의 분해와 에스토로겐 활성 제거

  • Kim, Yun-Jung (Division of Life Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Life Sciences, Kangwon National University) ;
  • Choi, Hyoung-T. (Division of Life Sciences, Kangwon National University)
  • 김윤정 (강원대학교 자연과학대학 생명과학부) ;
  • 송홍규 (강원대학교 자연과학대학 생명과학부) ;
  • 최형태 (강원대학교 자연과학대학 생명과학부)
  • Published : 2008.09.30
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Abstract

A white rot fungus Irpex lacteus produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, laccase, one of the lignin degrading enzymes, was too low to be assayed by spectrophotometry using o-tolidine as the chromogenic substrate in this fungus under various culture conditions. A laccase expression vector was constructed using a cDNA from Phlebia tremellosa with the constitutively expressed promoter of glyceraldehydes-3-phosphate dehydrogenase gene, and introduced into I. lacteus by the restriction enzyme mediated integration transformation through the protoplast-$CaCl_2$ procedure. Two transformants showed highly increased laccase activities at the early growth phase in the minimal liquid medium, and they not only degraded bisphenol A, a notorious endocrine disrupting chemical, but also removed the estrogenic activity effectively.

리그닌을 분해하는 백색부후균의 하나인 기계충버섯(Irpex lacteus)은 다양한 난분해성물질에 대한 분해능이 매우 높다. 그러나 이 균은 다양한 배양조건에서도 리그닌 분해효소의 하나인 laccase 활성이 매우 낮다. 난분해성 물질들에 대한 분해능을 향상시키기 위하여 laccase 활성을 증가시키고자 아교버섯의 laccase cDNA를 발현벡터로 구축하여 기계충버섯에 형질전환 방법으로 도입하였다. 항시 발현되는 glyceraldehyde-3-phosphate dehydrogenase 유전자의 프로모터와 재조합된 laccase 유전자는 생성된 형질전환체에서 배양초기인 생장기에서 강하게 발현되었으며, 생성된 형질전환체가 내분비장애물질의 하나인 비스페놀 A (BPA) 분해능은 물론 에스트로겐 활성 제거에 있어서도 더 우수하였다.

Keywords

References

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