미생물학회지 (Korean Journal of Microbiology)

  • 제44권2호
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  • Pages.135-139
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  • 2008
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

서울지역 급성위장관염 환자에서 검출된 노로바이러스의 유전자형 분포

Genetic Distribution of Human Noroviruses Detected from Acute Gastroenteritis Patients in Seoul

  • 김은정 (서울시보건환경연구원 바이러스검사팀) ;
  • 박상훈 (서울시보건환경연구원 바이러스검사팀) ;
  • 송미옥 (서울시보건환경연구원 바이러스검사팀) ;
  • 김무상 (서울시보건환경연구원 바이러스검사팀) ;
  • 김민영 (서울시보건환경연구원 바이러스검사팀) ;
  • 천두성 (질병관리본부 간염.폴리오팀) ;
  • 정혜숙 (서울시보건환경연구원 바이러스검사팀) ;
  • 김철중 (충남대학교 수의과대학)
  • Kim, Eun-Jeung (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Sang-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Song, Mi-Ok (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Moo-Sang (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Min-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Cheon, Doo-Sung (Korea Center for Disease Control & Prevention) ;
  • Jeong, Hae-Sook (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Chul-Joong (College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2008.06.30
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초록

2004년부터 207년까지 서울지역에서 산발적으로 발생한 급성위장관염 환자에 대하여 RT-PCR을 이용하여 노로바이러스를 검출하였다. 검출된 노로바이러스 258건에 대하여 염기서열분석을 실시한 결과 GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.8, GII.10, GII.12, GII.13, GII.15, GII.16, GII.17 등 13가지 유전자형이 검출되었으며 산발적으로 발생한 급성위장관염 환자에서 가장 많이 검출된 유전자형은 GII.4(76.7%)로 나타났다. 노로바이러스 유전자형의 다양성에 대한 연구로 서울지역 노로바이러스성 질환의 예방을 위한 기초자료로 활용될 것으로 기대된다.

Fecal specimens from acute gastroenteritis in Seoul from 2004 to 2007 were collected and then tested for the presence of norovirus by RT-PCR. 258 noroviruses were subjected to be futher characterized to sequencing analysis. The sequencing analysis showed that thirteen genotypes were detected (GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.8, GII.10, GII.l2, GII.13, GII.l5, GII.l6, GII.l7) and predominant genotype was GII.4 (76.7%) in the cases of norovirus detected sporadic acute gastroenteritis in Seoul. By this molecular investigation, genotypic distribution associated with norovirus infections will be used for control and prevention of norovirus related diseases.

키워드

참고문헌

  1. Ando, A., K. Mattick, D. Lewis, M. Estesw, X. Jiang, J. Green, R. Eglin, and D. Brown, 2000. Distinct epidemiology patterns of Norwalk-like virus infection. J. med. Virol. 62, 99-103 https://doi.org/10.1002/1096-9071(200009)62:1<99::AID-JMV15>3.0.CO;2-0
  2. Ando, T., M.N. Mulders, D.C. Lewis, M.K. Estes, S.S. Onroe, and R.I. Glass. 1994. Comparison of the polymerase region of small round structured virus strains previously classified in three antigenic types by solid-phase immune electron microscopy. Arch. Virol. 135, 217-226 https://doi.org/10.1007/BF01309781
  3. Atmar, R.L. and M.K. Estes. 2001. Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses. Clin. Microbiol. Rev. 14, 15-37 https://doi.org/10.1128/CMR.14.1.15-37.2001
  4. Dey, S.K., T.A. Nguyen, T.G. Phan, O. Nishio, A.F. Salim, M. Rahman, F. Yagyu, S. Okitsu, and H. Ushijima. 2007. Molecular and epidemiological trend of norovirus associated gastroenteritis in Dhaka City, Bangladesh. Clin. Virol. 9, 17
  5. Dingle, K.E., P.R. Lambden, E.O. Caul, and I.N. Clarke. 1995. Human enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus. J. Gen. Virol. 76, 2349-2355 https://doi.org/10.1099/0022-1317-76-9-2349
  6. Fang, Z.Y., H.P. Xie, H.X. Lv, Q. Zhang, Z.J. Duan, D. Steele, B. Jiang, and X. Jiang. 2007. Investigation of human calicivirus (HuCV) diarrhea among infantile and young children in China, 1999-2005. Bing. Du. Xue. Bao. 23, 5-9
  7. Gomes, K.A., J.A. Stupka, J. Gomez, and G.I. Parra. 2007. Molecular characterization of calicivirus strains detected in outbreaks of gastroenteritis in Argentina. J. Med. Virol. 79, 1703-1709 https://doi.org/10.1002/jmv.20989
  8. Green, S.M., K.E. Dingle, P.R. Lambdeb, E.O. Caul, C.R. Ashley, and I.N. Clarke. 1994. Human enteric Caliciviridae: a new prevalent SRSV group defined by RNA-dependent RNA polymerase and capsid diversity. J. Gen. Virol. 75, 1883-1888 https://doi.org/10.1099/0022-1317-75-8-1883
  9. Guntapong, R., G.S. Hansman, T. Oka, S. Ogawa, T. Kageyama, Y. Pongsuwanna, and K. Katayama. 2004. Norovirus and sapovirus infections in Thailand. Jpn. J. Infect. Dis. 57, 276-278
  10. Hale, A., S.E. Crawford, M. Ciarlet, J. Green, C.I. Gallimore, D.W.G. Brown, X. Jiang, and M.K. Estes. 1999. Expression and self-assembly of Grimsby virus: antigenic distinction from Norwalk virus and Mexico viruses. Clin. Diagn. Lab. Immunol. 6, 142-146
  11. Hansman, G.S., L.T. Doan, T.A. Kguyen, S. Okitsu, K. Katayama, S. Ogawa, K. Natori, N. Takeda, Y. Kato, O. Nishio, M. Noda, and H. Ushijima. 2004a. Detection of norovirus and sapovirus infection among children with gastroenteritis on Ho Chi Minh City, Vietnam. Arch. Virol. 149, 1673-1688
  12. Hansman, G.S., K. Katayama, N. Maneekarn, S. Peerakome, P. Khamrin, S. Tonusin, S. Okitsu, O. Nishio, N. Takeda, and H. Ushijia. 2004b. Genetic diversity of norovirus and sapovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand. J. Clin. Microbiol. 42, 1305-1307 https://doi.org/10.1128/JCM.42.3.1305-1307.2004
  13. Ho, E.C., P.K. Cheng, A.W. Lau, A.H. Wong, and W.W. Lim. 2007. Atypical norovirus epidemic in Hong Kong during summer of 2006 caused by a new genogroup II/4 variant. J. Clin. Microbiol. 45, 2205-2211 https://doi.org/10.1128/JCM.02489-06
  14. Jia, L.P., Y. qian, Y. Zhang, D.M. Chen, Y. Gao, and Q. Xu. 2007. Norovirus associated outbreaks of acute gastroenteritis in hospitals in Beijing in late 2006. Zhonghua Liu Xing Bing Xue Za Zhi. 28, 213-217
  15. Jiang, X., D. Cubitt, J. Hu, X. Dai, J. Treanor, D.O. Matson, and L.K. Pickering. 1995. Development of an ELISA to detect MX virus, a human calicivirus in the Snow Mount agent group. J. Gen. Virol. 76, 2739-2747 https://doi.org/10.1099/0022-1317-76-11-2739
  16. Jiang, X., D.O. Matson, W.D. Cubitt, and M.K. Estes. 1996. Genetic and antigenic diversity of human caliciviruses (HuCVs) using RT-PCR and new EIAs. Arch. Viol. 12, 251-262
  17. Jiang, X., J. Wang, D.Y. Graham, and M.K. Estes. 1992. Detection of norwalk virus in stool by polymerase chain reaction. J. Clin. Microbiol. 30, 2529-2534
  18. Jiang, X., N. Wilton, W.M. Zhang, T. Farkas, P.W. Huang, E. Barrett, M. Guerrero, G. Ruiz-Palacios, K.Y. Green, J. Green, A.D. Hale, M.K. Estes, L.K. Pickering, and D.O. Matson. 2000. Diagnosis of human Caliciviruses by use of enzyme immunoassays. J. Infect. Dis. 181, 349-359 https://doi.org/10.1086/315203
  19. Kageyama, T., M. Shinohara, K. Uchida, S. Fukushi, F.B. Hoshino, S. Kojima, R. Takai, T. Oka, N. Takeda, and K. Katayama. 2004. Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan. J. Clin. Microbiol. 42, 2988-2995 https://doi.org/10.1128/JCM.42.7.2988-2995.2004
  20. Katayama, K., H. Shirato-Horikoshi, S. Kojima, T. Kageyama, T. Oka, F.B. Hoshino, S. Fukushi, M. Shinohara, K. Uchida, Y. Suzuki, T. Gojobori, and N. Takeda. 2002. Phylogenetic analysis of the complete genome of 18 Norwalk-like viruses. J. Virol. 299, 225-239 https://doi.org/10.1006/viro.2002.1568
  21. Lambden, P.R., E.O. Caul, C.R. Ashley, and I.N. Clarke. 1993. Sequence and genome organization of a human small round-structured virus. Science 259, 516-519 https://doi.org/10.1126/science.8380940
  22. Leite, J.P.G., T. Ando, J.S. Noel, B. Jiang, C.D. Humphrey, J.F. Lew, K.Y. Green, R.I. Glass, and S.S. Monroe. 1996. Characterization of Toronto virus capsid protein expressed in baculovirus. Arch. Virol. 141, 865-875 https://doi.org/10.1007/BF01718161
  23. Okada, M., T. Ogawa, H. Yoshizumi, H. Kubonoya, and K. Shinozaki. 2007. Genetic variation of the norovirus GII-4 genotype associated with a large number of outbreaks on Chiba prefecture, Japan. Arch. Virol. 14, 56-59
  24. Phan, T.G., K. Kaneshi, Y. Usda, S. Nakaya, S. Nishimura, A. Yamamoto, K. Sugita, S. Takanashi, S. Okitsu, and H. Ushijima. 2007. Genetic heterogeneity, evolution, and recombination in noroviruses. J. Med. Virol. 79, 1388-1400 https://doi.org/10.1002/jmv.20924
  25. Shiota, T., M. Okame, S. Takanashi, P. Hhamrin, M. Takagi, K. Satou, Y. Masuoka, F. Yagyu, Y. Shimizu, H. Kohno, M. Mizuguchi, S. Ohitsu, and H. Ushijima. 2007. Characterization of broadly reactive monoclonal antibody against norovirus genogroup I and II: Recognition of a Novel conformational Epitope. J. Virol. 81, 12298-12306 https://doi.org/10.1128/JVI.00891-07
  26. Siebenga, J.J., H. Vennema, E. Duize, and M.P.G. Koopmans. 2007. Gastroenteritis caused by norovirus GGII.4, the Netherlands, 1994-2005. Emerg. Infect. Dis. 13, 144-146
  27. Wu, F.T., T. Oka, K. Katayama, H.S. Wu, Donald, D.S. Jiang, T. Miyamura, N. Takeda, and G.S. Hansman. 2006. Genetic diversity of noroviruses in Taiwan between November 2004 and March 2005. Arch. Virol. 151, 1319-1327 https://doi.org/10.1007/s00705-005-0717-4
  28. Yoon, J.S., S.G. Lee, S.K. Hong, S.A. Lee, W.H. Jheong, S.S. Oh, M.H. Oh, G.P. Ko, C.H. Lee, and S.Y. Paik. 2008. Molecular epidemiology of norovirus infections in children with acute gastroenteritis in South Korea in November 2005 through November 2006. J. Clin. Microbiol. 46, 1474-1477 https://doi.org/10.1128/JCM.02282-07