Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Synergistic Effects of Carboxymethylchitosan Fabric and Low Molecular Weight Heparin in Reducing Adhesion Formation in the Rat Uterine Horn Model

Rat에서 Carboxymethylchitosan Fabric과 Low Molecular Weight Heparin의 자궁각 유착 형성 방지 상승효과

  • Kwon, Young-Sam (College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, Kwang-Ho (School of Veterinary Medicine, University of Wisconsin-Madison)
  • 권영삼 (경북대학교 수의과대학) ;
  • 장광호 (위스콘신-매디슨 대학교 수의과대학)
  • Published : 2008.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to compare the efficacy of carboxymethylchitosan fabric (CMCF) with that of the combination of CMCF and low molecular weight heparin (LMWH) for the prevention of postoperative uterine adhesion in rats. Adhesions were induced by suturing both the uterine serosa and peritoneum abrased until petechial bleeding occurred. Fourteen days later, adhesions were evaluated clinically and histopathologically. The mean tensile strength was significantly decreased in the CMCF and CMCF+LMWH groups compared to that of control group, and the CMCF+LMWH group had the lowest tensile strength. The distance of adhesion site was highest in the CMCF group and slightly decreased in the CMCF+LMWH group comparing to that of control group. The inflammatory cell infiltration and neovascularization of the CMCF group were significantly lower than those of the control group. It was observed that the damage at intestinal serosa was significantly decreased in the CMCF+LMWH group comparing to that of control group. Therefore, it was concluded that CMCF and LMWH may be useful to prevent postoperative uterine adhesion in rats.

Keywords

References

  1. Al-Chalabi HA, Otubo JA. Value of a single intraperitoneal dose of heparin in prevention of adhesion formation: an experimental evaluation in rats. Int J Fertil. 1987; 32: 332-335
  2. Andrade-Gordon P, Strickland S. Interaction of heparin with plasminogen activators and plasminogen: effects on the activation of plasminogen. Biochemistry. 1986;25: 4033-4040 https://doi.org/10.1021/bi00362a007
  3. Bakkum EA, van Blitterswijk CA, Dalmeijer RA, Trimbos JB. A semiquantitative rat model for intraperitoneal postoperative adhesion formation. Gynecol Obstet Invest. 1994; 37: 99-105 https://doi.org/10.1159/000292534
  4. Basbug M, Aygen E, Tayyar M, Kaya E, Narin F, Oktem O. Hyaluronic acid plus heparin for improved efficacy in prevention of adhesion formation in rat uterine horn model. Eur J Obstet Gynecol Reprod Biol. 1998; 78: 109-112 https://doi.org/10.1016/S0301-2115(98)00019-0
  5. Becker JM, Dayton MT, Fazio VW, Beck DE, Stryker SJ, Wexner SD, Wolff BG, Roberts PL, Smith LE, Sweeney SA, Moore M. Prevention of postoperative abdominal adhesions by a sodium hyaluronate-based bioresorbable membrane: a prospective, randomized, double-blind multicenter study. J Am Coll Surg. 1996;183: 297-306
  6. Best CL, Rittenhouse D, Vasquez C, Norng T, Subias E, Sueldo CE. Evaluation of interceed(TC7) for reduction of postoperative adhesions in rabbits. Fertil Steril. 1992; 58: 817-820 https://doi.org/10.1016/S0015-0282(16)55333-3
  7. Borten M, Seibert CP, Taymor ML. Recurrent anaphylactic reaction to intraperitoneal dextran 75 used for prevention of postsurgical adhesions. Obstet Gynecol. 1983; 61: 755-757
  8. Bothin C. Counteracting postsurgical adhesions--the effect of combining oxidized regenerated cellulose and tissue plasminogen activator. Int J Fertil Menopausal Stud. 1995; 40: 102-105
  9. Burns JW, Skinner K, Colt MJ, Burgess L, Rose R, Diamond MP. A hyaluronate based gel for the prevention of postsurgical adhesions: evaluation in two animal species. Fertil Steril. 1996; 66: 814-821 https://doi.org/10.1016/S0015-0282(16)58642-7
  10. diZerega GS. Contemporary adhesion prevention. Fertil Steril. 1994; 61: 219-235 https://doi.org/10.1016/S0015-0282(16)56507-8
  11. Freedman MD, Leese P, Prasad R, Hayden D. An evaluation of the biological response to Fraxiparine, (a low molecular weight heparin) in the healthy individual. J Clin Pharmacol. 1990; 30: 720-727 https://doi.org/10.1002/j.1552-4604.1990.tb03633.x
  12. Fukasawa M, Girgis W, diZerega GS. Inhibition of postsurgical adhesions in a standardized rabbit model: II. Intraperitoneal treatment with heparin. Int J Fertil. 1991; 36: 296-301
  13. Golan A, Maymon R, Winograd I, Bukovsky I. Prevention of post-surgical adhesion formation using aspirin in a rodent model: a preliminary report. Hum Reprod. 1995; 10: 1797-1800 https://doi.org/10.1093/oxfordjournals.humrep.a136177
  14. Harris ES, Morgan RF, Rodeheaver GT. Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents. Surgery. 1995; 117: 663-669 https://doi.org/10.1016/S0039-6060(95)80010-7
  15. Hill-West JL, Chowdhury SM, Dunn RC, Hubbell JA. Efficacy of a resorbable hydrogel barrier, oxidized regenerated cellulose, and hyaluronic acid in the prevention of ovarian adhesions in a rabbit model. Fertil Steril. 1994; 62: 630-634 https://doi.org/10.1016/S0015-0282(16)56956-8
  16. Holtz G. Current use of ancillary modalities for adhesion prevention. Fertil Steril. 1985; 44: 174-176 https://doi.org/10.1016/S0015-0282(16)48730-3
  17. Kennedy R, Costain DJ, McAlister VC, Lee TD. Prevention of experimental postoperative peritoneal adhesions by N,O-carboxymethyl chitosan. Surgery. 1996; 120: 866-870 https://doi.org/10.1016/S0039-6060(96)80096-1
  18. Kwon YS, Jang IH. Adhesion prevention in the dog with sodium carboxymethylcellulose and low molecular weight heparin. Korean J Vet Clin Med. 1999; 16: 50-56
  19. Kwon YS, Jang HS, Jeong JH, Bae JS, Rhee JW, Kang HS, Yang JH, Li WX, Jang KH. Effect of high concentration of carboxymethyl chitosan on preventing formation of peritoneal adhesion in rats. Korean J Vet Res. 2003; 43: 151-156
  20. Markwardt F, Klocking HP. Heparin-induced release of plasminogen activator. Haemostasis. 1977; 6: 370-374
  21. Menzies D, Ellis H. Intestinal obstruction from adhesions--how big is the problem? Ann R Coll Surg Engl. 1990; 72: 60-63
  22. Operative Laparoscopy Study Group.Postoperative adhesion development after operative laparoscopy: evaluation at early second-look procedures. Fertil Steril. 1991; 55: 700-704 https://doi.org/10.1016/S0015-0282(16)54233-2
  23. Orita H, Campeau JD, Gale JA, Nakamura RM, diZerega GS. Differential secretion of plasminogen activator activity by postsurgical activated macrophages. J Surg Res. 1986; 41: 569-573 https://doi.org/10.1016/0022-4804(86)90080-6
  24. Reijnen MM, Meis JF, Postma VA, van Goor H. Prevention of intra-abdominal abscesses and adhesions using a hyaluronic acid solution in a rat peritonitis model. Arch Surg. 1999; 134: 997-1001 https://doi.org/10.1001/archsurg.134.9.997
  25. Sahin Y, Saglam A. Synergistic effects of carboxymethylcellulose and low molecular weight heparin in reducing adhesion formation in the rat uterine horn model. Acta Obstet Gynecol Scand. 1994; 73: 70-73 https://doi.org/10.3109/00016349409013399
  26. Sanfilippo JS, Cox JG, Nealon NA, Barrows GH. Comparison of corticosteroid therapy in the prevention of pelvic tissue reaction and adhesion formation. Int J Fertil. 1986; 30: 57-61
  27. Szabo A, Haj M, Waxsman I, Eitan A. Evaluation of seprafilm and amniotic membrane as adhesion prophylaxis in mesh repair of abdominal wall hernia in rats. Eur Surg Res. 2000; 32: 125-128 https://doi.org/10.1159/000008751
  28. The European Fraxiparin Study (EFS) Group. Comparison of a low molecular weight heparin and unfractionated heparin for the prevention of deep vein thrombosis in patients undergoing abdominal surgery. Br J Surg. 1988; 75: 1058-1063 https://doi.org/10.1002/bjs.1800751105
  29. Ueno H, Yamada H, Tanaka I, Kaba N, Matsuura M, Okumura M, Kadosawa T, Fujinaga T. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials. 1999; 20: 1407-1414 https://doi.org/10.1016/S0142-9612(99)00046-0
  30. Weibel MA, Majno G. Peritoneal adhesions and their relation to abdominal surgery. A postmortem study. Am J Surg. 1973; 126: 345-353 https://doi.org/10.1016/S0002-9610(73)80123-0