Journal of Korean Neurosurgical Society

  • 제44권4호
  • /
  • Pages.249-255
  • /
  • 2008
  • /
  • 2005-3711(pISSN)
  • /
  • 1598-7876(eISSN)
대한신경외과학회 (The Korean Neurosurgical Society)
권호 표지
DOI QR코드

DOI QR Code

The Neovascularization Effect of Bone Marrow Stromal Cells in Temporal Muscle after Encephalomyosynangiosis in Chronic Cerebral Ischemic Rats

  • Kim, Hyung-Syup (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea) ;
  • Lee, Hyung-Jin (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea) ;
  • Yeu, In-Seung (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea) ;
  • Yi, Jin-Seok (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea) ;
  • Yang, Ji-Ho (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea) ;
  • Lee, Il-Woo (Department of Neurosurgery, Daejeon St. Mary's Hospital, The Catholic University of Korea)
  • 발행 : 2008.10.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective : In Moyamoya disease, the primary goal of treatment is to improve collateral circulation through angiogenesis. In the present study, we obtained and sub-cultured bone marrow stromal cells (BMSCs) from rats without a cell-mediated immune response. Then, we injected the labeled BMSCs directly into adjacent temporal muscle during encephalomyosynangiosis (EMS). Three weeks after BMSC transplantation, we examined the survival of the cells and the extent of neovascularization. Methods : We divided 20 rats into a BMSC transplantation group (n=12) and a control group (n=8). Seven days after the induction of chronic cerebral ischemia, an EMS operation was performed, and labeled BMSCs ($1{\times}106^6/100\;{\mu}L$) were injected in the temporal muscle for the transplantation group, while an equivalent amount of culture solution was injected for the control group. Three weeks after the transplantation, temporal muscle and brain tissue were collected for histological examination and western blot analysis. Results : The capillary/muscle ratio in the temporal muscle was increased in the BMSC transplantation group compared to the control group, showing a greater increase of angiogenesis (p<0.05). In the brain tissue, angiogenesis was not significantly different between the two groups. The injected BMSCs in the temporal muscle were vascular endothelial growth factor (VEGF)-positive by immunofluorescence staining. In both temporal muscle and brain tissue, the expression of VEGF by western blot analysis was not much different between the two groups. Conclusion : During EMS in a chronic cerebral ischemia rat model, the injection of BMSCs resulted in accelerated angiogenesis in the temporal muscle compared to the control group.

키워드

참고문헌

  1. Asahara T, Masuda H, Takahashi T, Kalka C, Pastore C, Silver M, et al : Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res 85 : 221-228, 1999 https://doi.org/10.1161/01.RES.85.3.221
  2. Assmus B, Schachinger V, Teupe C, Britten M, Lehmann R, Dobert N, et al : Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCAREAMI). Circulation 106 : 3009-3017, 2002 https://doi.org/10.1161/01.CIR.0000043246.74879.CD
  3. Bang OY, Lee JS, Lee PH, Lee G : Autologous mesenchymal stem cell transplantation in stroke patients. Ann Neurol 57 : 874-882, 2005 https://doi.org/10.1002/ana.20501
  4. Chu K, Park KI, Lee ST, Jung KH, Ko SY, Kang L, et al : Combined treatment of vascular endothelial growth factor and human neural stem cells in experimental focal cerebral ischemia. Neurosci Res 53 : 384-390, 2005 https://doi.org/10.1016/j.neures.2005.08.010
  5. Grunewald M, Avraham I, Dor Y, Bachar-Lustig E, Itin A, Jung S, et al : VEGF-induced adult neovascularization : recruitment, retention, and role of accessory cells. Cell 124 : 175-189, 2006 https://doi.org/10.1016/j.cell.2005.10.036
  6. Hai J, Li ST, Lin Q, Pan QG, Gao F, Ding MX : Vascular endothelial growth factor expression and angiogenesis induced by chronic cerebral hypoperfusion in rat brain. Neurosurgery 53 : 963-970, 2003 https://doi.org/10.1227/01.NEU.0000083594.10117.7A
  7. Harrigan MR, Ennis SR, Masada T, Keep RF : Intraventricular infusion of vascular endothelial growth factor promotes cerebral angiogenesis with minimal brain edema. Neurosurgery 50 : 589-598, 2002 https://doi.org/10.1097/00006123-200203000-00030
  8. Hayashi T, Abe K, Suzuki H, Itoyama Y : Rapid induction of vascular endothelial growth factor gene expression after transient middle cerebral artery occlusion in rats. Stroke 28 : 2039-2044, 1997 https://doi.org/10.1161/01.STR.28.10.2039
  9. Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, et al : Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell 109 : 625-637, 2002 https://doi.org/10.1016/S0092-8674(02)00754-7
  10. Kang HJ, Kim HS, Zhang SY, Park KW, Cho HJ, Koo BK, et al : Effects of intracoronary infusion of peripheral blood stem-cells mobilised with granulocyte-colony stimulating factor on left ventricular systolic function and restenosis after coronary stenting in myocardial infarction : the MAGIC cell randomised clinical trial. Lancet 363 : 751-756, 2004 https://doi.org/10.1016/S0140-6736(04)15689-4
  11. Kim SW, Han H, Chae GT, Lee SH, Bo S, Yoon JH, et al : Successful stem cell therapy using umbilical cord blood-derived multipotent stem cells for Buerger's disease and ischemic limb disease animal model. Stem Cells 24 : 1620-1626, 2006 https://doi.org/10.1634/stemcells.2005-0365
  12. Kirana S, Stratmann B, Lammers D, Negrean M, Stirban A, Minartz P, et al : Wound therapy with autologous bone marrow stem cells in diabetic patients with ischaemia-induced tissue ulcers affecting the lower limbs. Int J Clin Pract 61 : 690-692, 2007 https://doi.org/10.1111/j.1742-1241.2007.01303.x
  13. Kovacs Z, Ikezaki K, Samoto K, Inamura T, Fukui M : VEGF and flt. Expression time kinetics in rat brain infarct. Stroke 27 : 1865-1872, 1996 https://doi.org/10.1161/01.STR.27.10.1865
  14. Kusaka N, Sugiu K, Tokunaga K, Katsumata A, Nishida A, Namba K, et al : Enhanced brain angiogenesis in chronic cerebral hypoperfusion after administration of plasmid human vascular endothelial growth factor in combination with indirect vasoreconstructive surgery. J Neurosurg 103 : 882-890, 2005 https://doi.org/10.3171/jns.2005.103.5.0882
  15. Marti HJ, Bernaudin M, Bellail A, Schoch H, Euler M, Petit E, et al : Hypoxia-induced vascular endothelial growth factor expression precedes neovascularization after cerebral ischemia. Am J Pathol 156 : 965-976, 2000 https://doi.org/10.1016/S0002-9440(10)64964-4
  16. Miki Y, Nonoguchi N, Ikeda N, Coffin RS, Kuroiwa T, Miyatake S : Vascular endothelial growth factor gene-transferred bone marrow stromal cells engineered with a herpes simplex virus type 1 vector can improve neurological deficits and reduce infarction volume in rat brain ischemia. Neurosurgery 61 : 586-594, 2007 https://doi.org/10.1227/01.NEU.0000290907.30814.42
  17. Paxinos G, Watson C : The rat brain in stereotaxic coordinates, 4th ed. UK . Academic Press, 1998
  18. Renault MA, Losordo DW : Therapeutic myocardial angiogenesis. Microvasc Res 74 : 159-171, 2007 https://doi.org/10.1016/j.mvr.2007.08.005
  19. Shweiki D, Itin A, Soffer D, Keshet E : Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature 359 : 843-851, 1992 https://doi.org/10.1038/359843a0
  20. Strauer BE, Brehm M, Zeus T, Kostering M, Hernandez A, Sorg RV, et al : Repair of infarcted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans. Circulation 106 : 1913-1918, 2002 https://doi.org/10.1161/01.CIR.0000034046.87607.1C
  21. Tse HF, Kwong YL, Chan JK, Lo G, Ho CL, Lau CP : Angiogenesis in ischaemic myocardium by intramyocardial autologous bone marrow mononuclear cell implantation. Lancet 361 : 47-49, 2003 https://doi.org/10.1016/S0140-6736(03)12111-3
  22. Wang YQ, Guo X, Qiu MH, Feng XY, Sun FY : VEGF overexpression enhances striatal neurogenesis in brain of adult rat after a transient middle cerebral artery occlusion. J Neurosci Res 85 : 73-82, 2007 https://doi.org/10.1002/jnr.21091
  23. Woodbury D, Schwarz EJ, Prockop DJ, Black IB : Adult rat and human bone marrow stromal cells differentiate into neurons. J neuroscience Res 61 : 364-370, 2000 https://doi.org/10.1002/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C
  24. Yeu IS, Lee HJ, Yi JS, Yang JH, Lee IW, Lee HK : The survival and migration pattern of the bone marrow stromal cells after intracerebral transplantation in rats. J Korean Neurosurg Soc 36 : 400-404, 2004
  25. Zentilin L, Tafuro S, Zacchigna S, Arsic N, Pattarini L, Sinigaglia M, et al : Bone marrow mononuclear cells are recruited to the sites of VEGF-induced neovascularization but are not incorporated into the newly formed vessels. Blood 107 : 3546-3554, 2006 https://doi.org/10.1182/blood-2005-08-3215
  26. Zhu W, Mao Y, Zhao Y, Zhou LF, Wang Y, Zhu JH, et al : Transplantation of vascular endothelial growth factor-transfected neural stem cells into the rat brain provides neuroprotection after transient focal cerebral ischemia. Neurosurgery 57 : 325-333, 2005 https://doi.org/10.1227/01.NEU.0000166682.50272.BC

피인용 문헌

  1. Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering vol.6, pp.None, 2008, https://doi.org/10.2147/ijn.s25297
  2. Serial Expression of Hypoxia Inducible Factor-$1{\alpha}$ and Neuronal Apoptosis in Hippocampus of Rats with Chronic Ischemic Brain vol.50, pp.6, 2008, https://doi.org/10.3340/jkns.2011.50.6.481
  3. Synthesis and characterization of injectable, thermosensitive, and biocompatible acellular bone matrix/poly(ethylene glycol)‐poly (ε‐caprolactone)‐poly(ethylene glycol) hydro vol.a100, pp.1, 2008, https://doi.org/10.1002/jbm.a.33262
  4. Role of a Burr Hole and Calvarial Bone Marrow-Derived Stem Cells in the Ischemic Rat Brain : A Possible Mechanism for the Efficacy of Multiple Burr Hole Surgery in Moyamoya Disease vol.58, pp.3, 2008, https://doi.org/10.3340/jkns.2015.58.3.167
  5. Combined gene therapy with vascular endothelial growth factor plus apelin in a chronic cerebral hypoperfusion model in rats vol.127, pp.3, 2008, https://doi.org/10.3171/2016.8.jns16366
  6. Combined gene therapy with vascular endothelial growth factor plus apelin in a chronic cerebral hypoperfusion model in rats vol.127, pp.3, 2008, https://doi.org/10.3171/2016.8.jns16366
  7. A refined model of chronic cerebral hypoperfusion resulting in cognitive impairment and a low mortality rate in rats vol.131, pp.3, 2019, https://doi.org/10.3171/2018.3.jns172274
  8. A refined model of chronic cerebral hypoperfusion resulting in cognitive impairment and a low mortality rate in rats vol.131, pp.3, 2019, https://doi.org/10.3171/2018.3.jns172274
  9. VEGF-PLGA controlled-release microspheres enhanced angiogenesis in encephalomyosynangiosis-based chronic cerebral hypoperfusion vol.81, pp.None, 2008, https://doi.org/10.1016/j.jocn.2020.09.023