Journal of Periodontal and Implant Science

  • 제38권3호
  • /
  • Pages.543-550
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  • 2008
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현

Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide

  • 김정은 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 이성훈 (서울대학교 치의학대학원 구강악안면 감염-면역학전공 및 치학연구소) ;
  • 최봉규 (서울대학교 치의학대학원 구강악안면 감염-면역학전공 및 치학연구소) ;
  • 구기태 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 김태일 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 이용무 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 구영 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 정종평 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 류인철 (서울대학교 치의학대학원 치주과학교실 및 치학연구소)
  • Kim, Jung-Eun (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Lee, Sung-Hoon (Oral Infection and Immunity, School of Dentistry, Seoul National University) ;
  • Choi, Bong-Kyu (Oral Infection and Immunity, School of Dentistry, Seoul National University) ;
  • Koo, Ki-Tae (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Tae-Il (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Ku, Young (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 발행 : 2008.09.30
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초록

Purpose: The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. Material and Methods: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria. Result: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-$1{\beta}$ was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs. Conclusion: The membrane protein of T. forsythia could be one of virulence factors.

키워드

참고문헌

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