Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Identification of Inflammation-related Genes Altered in the Cystic Ovary and Endometritis of Korean Cattle

난소낭종 및 자궁내막염 한우에서 염증유래 유전자 발굴

  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Park, Sun-Young (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Eun-Sook (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Moon, Yoon-Ja (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Park, Hye-Jin (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyun-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Bum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Park, Jae-Yong (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Hong, Seong-Geun (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Han, Jae-Hee (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kang, Da-Won (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University)
  • 최창용 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 박선영 (경상대학교 의과대학 생리학교실) ;
  • 김은숙 (경상대학교 의과대학 생리학교실) ;
  • 문윤자 (경상대학교 의과대학 생리학교실) ;
  • 박혜진 (경상대학교 의과대학 생리학교실) ;
  • 손동수 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 김재범 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 박재용 (경상대학교 의과대학 생리학교실) ;
  • 홍성근 (경상대학교 의과대학 생리학교실) ;
  • 한재희 (경상대학교 의과대학 생리학교실) ;
  • 강다원 (경상대학교 의과대학 생리학교실)
  • Published : 2008.09.30
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Abstract

This study was carried out to investigate inflammation-related gene expression altered in ovary and endometrium of Korean cattle with reproductive disorders using microarray. In the present study, nine inflammation-related differential1y expressed genes (DEGs) were identified in the cystic ovary and endometrium with endometritis. In the follicular cyst, eotaxin and alpha-2-HS-glycoprotein (AHSG) were up-regulated, whereas complement component 3 (C3) and oxidised low density lipoprotein (lectin-like) receptor 1 (OLR1) were down-regulated. Complement component 4A (C4A) was up-regulated in luteal cyst. In the endometritis, chemokine 1igand l and 2 (CXCL1 and CXCL2), protein C (inactivator of coagulation factors Va and VIIIa), and complement component C5 were up-regulated, whereas kininogen was down-regulated. Of these genes, we focused on eotaxin and kininogen, which were highly regulated in the follicular cyst and endometritis, respectively and on C3 commonly regulated in both reproductive disorders. The microarray data of eotaxin, kininogen, and C3 were validated by semi-quantitative PCR. Consistent with microarray data, eotaxin was up-regulated by 4-fold in the follicular cyst, while kininogen was down-regulated by 5-fold in the endometritis. C3 was down-regulated in the both follicular cyst and endometritis. Our results suggest that these inflammation-related genes could be useful markers for diagnosis of cystic ovary and endometritis of Korean cattle.

Keywords

References

  1. Allen MR, Zhang BR, Hettinger AM, Goad DW, Malayer JR and Geisert RD. 2002. Detection of bradykinin and bradykinin- $\{beta}2$ receptors in the porcine endometrium during the estrous cycle and early pregnancy. Biol. Reprod. 66:574-579 https://doi.org/10.1095/biolreprod66.3.574
  2. Bayer-Garner IB and Korourian S. 2001. Plasma cells in chronic endometritis are easily identified when stained with syndecan-1. Mod. Pathol. 14:877-879 https://doi.org/10.1038/modpathol.3880405
  3. Bhoola KD, Figueroa CD and Worthy K. 1992. Bioregulation of kinins: kalli-kreins, kininogens, and kininases. Pharmacol. Rev. 44:1-80
  4. Bartel J, Schluter C, Richter E, Noso N, Kulke R and Christophers E. 1996. Human dermal fibroblasts express eotaxin: molecular cloning, mRNA expression, and identification of eotaxin sequence variants. Biochem. Biophys. Res. Commun. 225: 1045-1051 https://doi.org/10.1006/bbrc.1996.1292
  5. Cairoli F. Battocchio M, Veronesi MC, Brambilla D, Conserva F, Eberini I, Wait R and Cianazza E. 2006. Serum protein during cow pregnancy: Acute-phase proteins increase in the peripartum period. Electrophoresis 27:1617-1625 https://doi.org/10.1002/elps.200500742
  6. Clements J. 1997. The molecular biology of the kallikreins and their roles in inflammation. In: Farmer G (ed), The Kinin System. London: Academic Press. 71-97
  7. Fabre J, Rivard A, Magne M, Silver M and Isner JM. 1999. Tissue inhibitions of angiotension-converting enzyme stimulates angiogenesis in vivo. Circulation 23:3043-3049
  8. Fischer C, Drillich M, Gabler C, Heuwieser W and Einspanier R. 2006. Postpartum reproductive failure in cattle: is the examination of gene expression in the endometrium a key to success? Berl. Munch. Tierarztl. Wochenschr. 119:197-202
  9. Figueroa CD, Chacon C, Corthorn J, Ehrenfeld P, Muller-Esterl W and Valdes G. 2001. Temporospatial changes of kinin B2 receptors during the estrous cycle and pregnancy in the rat uterus. Biol. Reprod. 62:1590-1599
  10. Fredslund F, Jenner L, Husted LB, Nyborg J, Andersen GR and Sottrup LJ. 2006. The structure of bovine complement component 3 reveals the basis for thioester function. J. Mol. Biol. 361:115-127 https://doi.org/10.1016/j.jmb.2006.06.009
  11. Fukuoka Y, Xia HZ, Sanchez-Muñoz LB, Dellinger AL, Escribano L and Schwartz LB. 2008. Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5. J. Immunol. 180:6307-6316 https://doi.org/10.4049/jimmunol.180.9.6307
  12. Gao X, Greenbaum LM, Mahesh VB and Brann DW. 1992. Characterization of the kinin system in the ovary during ovalation in the rat. Biol. Reprod. 47:945-951 https://doi.org/10.1095/biolreprod47.6.945
  13. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, Celestin J, Leder P and Luster AD. 1996. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nature Med. 2: 449-456 https://doi.org/10.1038/nm0496-449
  14. Dent G, Hadjicharalambous C, Yoshikawa T, Handy RLC, Powell J, Anderson JK, Louis R, Davies DE and Djukanovic R. 2004. Contribution of eotaxin-1 to eosinophil chemotactic activity of moderate and severe asthmatic sputum Am. J. Respir. Crit. Care. Med. 169:1110-1117 https://doi.org/10.1164/rccm.200306-855OC
  15. Hamilos DL, Leung DY, Wood R, Cunningham L, Bean DK, Yasruel Z, Schotman E and Hamid Q. 1995. Evidence for distinct cytokine expression in allergic versus nonallergic chronic sinusitis. J. Allergy Clin. Immunol. 96:537-544 https://doi.org/10.1016/S0091-6749(95)70298-9
  16. Jose PJ, Griffiths-Johnson DA, Collins PD, Walsh DT, Moqbel R, Totty NF, Truong O, Hsuan JJ and Williams TJ. 1994. Eotaxin: a potent eosinophil chemoattractant cytokine detected in a guinea-pig model of allergic airways inflammation. J. Exp. Med. 179:881-887 https://doi.org/10.1084/jem.179.3.881
  17. Kerr AR, Paterson GK, Riboldi AT and Mitchell TJ. 2005. Innate immune defense against pneumococcal pneumonia requires pulmonary complement component C3. Infection Immun. 73:4245-4252 https://doi.org/10.1128/IAI.73.7.4245-4252.2005
  18. Kihara T, Kimura A, Moriyama A, Ohkubo I and Takahashi T. 2000. Identification of components of the intrafollicular bradykinin-producing sys-tem in the porcine ovary. Biol. Reprod. 62:1160-1167 https://doi.org/10.1095/biolreprod62.5.1160
  19. Leung ST, Cheng Z, Sheldrick EL, Derecka K, Derecka K, Flint AP and Wathes DC. 2001. The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. J. Endocrinol. 168:497-508 https://doi.org/10.1677/joe.0.1680497
  20. Murone C, Chai SY, Muller-Estrel W, Mendelsohn FAO and Clements J. 1999. Localization of bradykinin $\beta$2 receptors in the endometrium and myo-metrium of rat uterus and effects of estrogen and progesterone. Endocrinology 140: 3372-3382. https://doi.org/10.1210/en.140.7.3372
  21. Rajapakse S, Yamano R, Ogiwara K, Hirata K, Takahashi S and Takahashi T. 2007. Estrogen-dependent expression of the tissue kallikrein Gene (KIk1) in the mouse uterus and its implications for endometrial tissue growth. Mol. Reprod. Dev. 74:1053-1063 https://doi.org/10.1002/mrd.20567
  22. Renne T, Dedio J, David G, and Mu¨ller-Esterl W. 2000. High molecular weight kininogen utilizes heparan sulfate proteoglycans for accumulation on endothelial cells. J. Biol. Chem. 275:33688-33696 https://doi.org/10.1074/jbc.M000313200
  23. Sayegh RA, Tao XJ, Awwad JT and Isaacson KB. 1996. Localization of the expression of complement component 3 in the human endometrium by in situ hbridization. J. Clin. Endocrinol. Metab. 81:1641-1649 https://doi.org/10.1210/jc.81.4.1641
  24. Valdes G, Figueroa CD and Corthorn J. 1996. Temporospatial changes of kal-likrein-like enzymes during the estrous cycle and pregnancy in the rat uterus. Biol. Reprod. 55:236-245 https://doi.org/10.1095/biolreprod55.2.236
  25. Vonnahme KA, Fernando SC, Ross JW, Ashworth MD, De-Silva U, Malayer JR and Geisert RD. 2004. Porcine endometrial expression of kininogen, factor Xll, and plasma kallikrein in cyclic and pregnant gilts. Biol. Reprod. 70:132-138
  26. Vonnahme KA, Malayer JR, Spivey HO, Ford SP, Clutter AC and Geisert RD. 1999. Detection of kallikrein gene expression and enzymatic activity in porcine endometreum during the estrous cycle and early pregnancy. Biol. Reprod. 61:1235-1241 https://doi.org/10.1095/biolreprod61.5.1235
  27. Yu XP and Chen ZL. 2004. Advances in research of complement component 3. Immunol. J. 20:483-486
  28. 최창용, 손동수, 최규찬, 최창열, 최선호, 김현종, 조상래, 허창기, 강다원, 2006. 한우번식우 사육 농가의 번식장애에 실태 조사. 한국수정란이식학회지 21:331-338