Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Expression and DNA Methylation Change of Oct-4 in Cloned Bovine Blastocysts

체세포복제 소 배반포의 Oct-4 발현과 DNA 메틸화 변화

  • 차병현 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 최정상 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 황성수 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 정학재 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 임기순 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 양병철 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 김명직 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 조재현 (경상대학교 수의학과) ;
  • 성환후 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 고응규 (농촌진흥청 축산과학원 응용생명공학과)
  • Published : 2008.09.30
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Abstract

DNA methylation is one of the reasons for poor survival of clone animals. The OCT-4 gene is essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that the 5'-promoter region of Oct-4 gene was a target of DNA methylation and the methylation status was changed variously during embryonic development in bovine. The study conducted to examine the expression and methylation pattern of tissue-dependent differentially methylated region (T-DMR) of Oct-4 gene in bovine somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF) blastocysts. The Oct-4 gene expression was evaluated by RT-PCR and fluorescence immunocytochemistry. The methylation pattern of T-DMR was analyzed using restriction mapping and bisulfite sequencing methods. The Oct-4 transcripts were highly expressed in IVF, while they were not expressed in SCNT. The Oct-4 protein was not detected or expressed at very low level in SCNT, the intensity of Oct-4 protein, however, was strong in IVF. On the other hand, the T-DMR of Oct-4 gene was hypermethylated in SCNT compared to that of IVF. These results suggested that expression and the failure of demethylation of Oct-4 gene was closely associated with incomplete development of SCNT embryos.

Keywords

References

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