Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of a NP Strain for Cucumber mosaic virus(CMV-NP) Identified Newly from Sweet Pepper Showing Fruit Necrosis

과일 괴저 병징의 단 고추에서 분류동정한 오이모자이크바이러스의 새로운 계통 CMV-NP 특성

  • 조점덕 (농촌진흥청 원예연구소 원예환경과) ;
  • 김정수 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 이중환 (경북농업기술원 환경농업연구과) ;
  • 정봉남 (농촌진흥청 원예연구소 원예환경과)
  • Published : 2008.08.01
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Abstract

A strain of Cucumber mosaic virus(CMV) was isolated newly from sweet pepper(Capsicum annuum var. angulosum) showing necrosis with large necrotic spots on fruits and vein banding with malformation on leaf at Cheongdo area in Gyeongsangbukdo. The new strain was designated as a CMV-NP and the shape of virus particles was isometric of 26 nm in diameter from the sweet pepper fruit by Dip method. The strain of CMV-NP was identified genetically by VC/RT-PCR. CMV-NP could infect systemically on the 9 indicator plants including Cucumis sativus, but it could infect locally on Chenopodim amaranticolor and C. quinoa. CMV-NP induced the specific symptoms of necrotic rings on the inoculated and the upper leaves of N. rustica and Tetragonia expansa. On Cucumis sativus, the large chlorotic ring and vein chlorosis were produced on the upper leaves. CMV-NP had no virulence on Datura stramonium.

경북 청도지역의 단 고추 과일에 나타난 큰 괴저 반점 및 과일 괴저와 잎에 엽맥 녹대 및 기형 병징을 일으키는 바이러스는 오이모자이크바이러스의 새로운 계통인 CMV-NP로 분류동정되었다. CMV-NP는 직경이 26 nm의 구형이었으며, VC/RT-PCR 유전자 진단결과 CMV로 확인되었다. CMV-NP는 오이(Cucumis sativus) 등 9개 지표식물에 전신감염을 일으켰으며, 명아주(Chenopodim amaranticolor; C. quinoa)에 국부감염을 일으켰다. CMV-NP는 담배(N. rustica)와 번행초(Tetragonia expansa)의 접종잎과 상엽에 특이한 괴저 원형 반점을 일으켰다. 특히 오이에서는 상엽에 큰 퇴록 원형반점과 엽맥퇴록 병징을 일으켰으며 독말풀(Datura stramonium)에서는 병원성이 없었다.

Keywords

References

  1. Cho, J. D., Kim, J. S., Kim, H. R., Chung, B. N. and Ryu, K. H. 2006a. Convenient nucleic acid detection for Tomato spotted wilt virus: Virion captured/RT-PCR (VC/ RT-PCR). Res. Plant Dis. 12: 139-143 https://doi.org/10.5423/RPD.2006.12.2.139
  2. Cho, J. D., Lee, S. H., Kim, J. S., Choi, G. S., Kim, H. R., Chung, B. N. and Rye, K. H. 2006b. Characteristics of Cucumber mosaic virus-VCH causing vein chlorosis on red pepper in Korea. Res. Plant Dis. 12: 226-230 https://doi.org/10.5423/RPD.2006.12.3.226
  3. Choi, G. S., Kim, J. H., Kim, J. S. and Choi, J. K. 2004. Characterization of Cucumber mosaic virus isolated from water chickweed. Plant Pathol. J. 20: 131-134 https://doi.org/10.5423/PPJ.2004.20.2.131
  4. Choi, G. S., Kim, J. H., Lee, D. H., Kim, J. S. and Ryu, K. H. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21: 258-261 https://doi.org/10.5423/PPJ.2005.21.3.258
  5. Choi, H. S., Ryu, J. K., Ahn, K. K., Cho, J. D. and Kim, J. S. 2001. Cucumber mosaic cucumovirus-CARNA5 causing bud necrosis on table tomato. Plant Pathol. J. 17: 169-173
  6. Choi, J. K., Kim, H. J., Hong, J. S., Kim, K. W. and Lee, S. Y. 1998. Identification of Cucumber mosaic virus isolates in Korea. Plant Pathol. J. 14: 7-12
  7. Kim, J. H., Choi, G. S. and Choi, J. K. 2002. Characterization of Cucumber mosaic virus subgroup II isolated from paprika in Korea. Plant Pathol. J. 18: 6-11 https://doi.org/10.5423/PPJ.2002.18.1.006
  8. Kim, J. S. and Choi, G. S. 2002. Viruses infecting pepper and their characteristics. J. Kor. Capsicum Res. Coop. 7: 23-47
  9. Kim, J. S. and Lee, K. H. 1991. Virus disease incidence and symptom expression of peppers cultivated in vinyl house, and serological relation ship of CMV isolates from peppers. Res. Rept. RDA (C.P) 33: 8-14
  10. Kim, J. S., Kim, S. K., Choi, G. S. and Lee, M. W. 1990. Virus disease incidence and symptom appearance in red pepper. Korean J. Plant Pathol. 6: 125-132

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