Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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The Influence of Bisphenol A on Selenoprotein N Expression Genes during Zebrafish Embryogenesis

Bisphenol A가 Zebrafish 발생과정 중 Selenoprotein N 발현 유전자에 미치는 영향

  • Gwak, Young-Gook (Department of Life Science and Biotechnology, Yonsei University) ;
  • Yeo, Min-Kyeong (Department of Environmental Science and Engineering, KyungHee University)
  • 곽영국 (연세대학교 생명과학공학부) ;
  • 여민경 (경희대학교 환경학 및 환경공학)
  • Published : 2008.09.30
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Abstract

내분비계 장애물질 중 에스트로겐성 특성을 지닌 것으로 알려진 bisphenol A에 폭로되었을 때에 나타나는 이상 증상과 selenoprotein N 결핍 증상은 유사성이 있는 것으로 관찰되었다. 이에 본 연구에서는 bisphenol A에 폭로된 생물체의 selenoprotein N 유전자 발현에 영향을 미치는지에 관해 연구하였다. 실험동물은 zebrafish (Danio rerio, wild type)의 수정란을 사용하였다. Zebrafish의 수정란은 서로 다른 농도의 bisphenol A (0.1, 1, 10 ppm)에 노출하였다. 각각의 폭로 환경에서 부화된 치어를 시료로 selenoprotein N 유전자 발현을 RT-PCR방법에 의해 알아보았다. 그 결과, bisphenol A는 척추이상과 심장이상이 나타나 selenoprotein N 이상시 나타나는 현상과 유사성을 보였다. Selenoprotein N 유전자 발현은 bisphenol A 폭로 농도가 높아짐에 따라 유전자 발현 농도가 낮아지는 것으로 관찰되었다. 특히 1 ppm 농도에서는 대조군이나 0.1 ppm 농도보다 catalase의 활성이 높게 나타나 특정 농도에서 bisphenol A에 의한 영향이 나타나는 것을 알 수 있었다.

Keywords

References

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