The Journal of Internal Korean Medicine (대한한방내과학회지)
- Volume 28 Issue 3
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- Pages.473-491
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- 2007
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- 1226-9174(pISSN)
Induction of Apoptosis by Samgibopae-tang in Human Non-small-cell Lung Cancer Cells
인체폐암세포 NCI-H460 및 A549의 apoptosis 유발에 미치는 삼기보배탕의 영향
- Heo, Man-Kyu (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Heo, Tae-Yool (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Kim, Ki-Tak (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Byun, Mi-Kwon (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Kim, Jin-Young (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Sim, Sung-Heum (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Kim, Koang-Lock (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Kam, Cheol-Woo (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University) ;
- Park, Dong-Il (Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University)
- 허만규 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 허태율 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 김기탁 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 변미권 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 김진영 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 심성흠 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 김광록 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 감철우 (동의대학교 부속한방병원 폐계내과학 교실) ;
- 박동일 (동의대학교 부속한방병원 폐계내과학 교실)
- Published : 2007.09.30
Abstract
Objectives : This study was designed to investigate the antiproliferative activity of the water extract of Samgibopae-tang (SGBPT) in NCI-H460 and A549 non-small-cell lung cancer cell lines Methods : In this study, we measured the subsistence, form of NCI-H460 and A549 non-small-cell lung cancer cell by hemocytometer and DAPI staining. In each cell, we analyzed DNA fragmentation. reverse transcription-polymerase chain reaction and measured activity of caspase-3, caspase-8 and caspase-9. Results and Conclusions : We found that exposure of A549 cells to SGBPT resulted in growth inhibition in a dose-dependent manner. butSGBPT did not affect the growth of NCI-H460 cells. The antiproliferative effect by SGBPT treatment in A549 cells was associated with morphological changes. SGBPT treatment partially induced the expression of DR5 cells and the expression of Faswas markedly increased in both transcriptional and translational levels in A549 cells. SGBPT treatment partially induced the expression of Bcl-2, Bcl-XL and the expression of Bid was markedly decreased in translational levels in A549 cells. However, SGBPT treatment did not affect the expression of IAP family in A549 orNCI-H460 cells. SGBPT treatment partially induced the expression of caspase-3, caspase-8, caspase-9 activity which markedly increased in a dose-dependent manners in A549 cells. The fragmental development of PARP and