Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory Effect of Yongseollan on the LPS-activated RAW 264.7 Cells

  • Jo, Mi-Jeong (College of Oriental Medicine and Development Team for The New Drug of Oriental Medicine (BK21 program), Daegu Haany University) ;
  • Lee, Byung-Wook (College of Oriental Medicine, Daegu Haany University) ;
  • Eom, Dong-Myung (Korea Institute of Oriental Medicine) ;
  • Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University) ;
  • Hwangbo, Min (College of Oriental Medicine, Daegu Haany University) ;
  • Jee, Seon-Young (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Oriental Medicine and Development Team for The New Drug of Oriental Medicine (BK21 program), Daegu Haany University)
  • Published : 2007.06.30
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Abstract

This study was conducted to evaluate the inhibitory effects of Yongseollan(YSL) on the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated RAW264.7 cells. YSL is tropical plant originating from Mexico. The biological activity of this plant is not yet evaluated systematically. The aim of the present work is to investigate a potential anti-inflammatory activity of YSL. The RAW264.7 cells were cultured in D MEM/F12 medium for 24 hrs. After serum starvation, cells were treated with YSL for 1 hr, followed by stimulating NO production with a LPS. We found that YSL has an inhibitory effect on the production of NO, iNOS expression and $phospho-I{\kappa}B$ expression. YSL also inhibited tumor necrosis factor $(TNF)-{\alpha}$, interleukin (IL)-6, and $IL-1{\beta}$. Moreover, YSL inhibited cyclooxygenase (COX)-2 expression and prostanglandin E2 (PGE2). These findings showed that YSL could have some anti-inflammatory effects which might play a role in therapy in Gram-negative bacterial infections.

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