The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Euphorbiae lathyridis Semen on cell apoptosis in HT-29 human colon cancer cells

속수자가 HT-29 대장암세포의 활성 및 세포사멸에 미치는 영향

  • Lee, Jae-Hyun (Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Jung, Sun-Ju (Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Park, Yong-Ki (Dept. of Herbology, College of Oriental Medicine, Dongguk University)
  • 이제현 (동국대학교 한의학대학 본초학교실) ;
  • 정선주 (동국대학교 한의학대학 본초학교실) ;
  • 박용기 (동국대학교 한의학대학 본초학교실)
  • Published : 2007.06.30
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Abstract

Objectives : In this study, we investigate that Euphorbiae lathyridis Semen extract contributes to growth inhibitory effect and anti-cancer activity on the HT-29 human colon cancer cells. Methods : Euphorbiae lathyridis Semen was extracted from the Semen of the plant using 80% Methanol. The Euphorbiae lathyridis Semen extract was treated to different concentrations for 24 hr, 4Shr or 72hr. Growth inhibitory effect was analyzed by measuring FACS study and MTT assay. Cell apoptosis was confirmed by surveying caspases cascades activation using Westem blot. Results : Exposure to Euphorbiae lathyridis Semen extract (0.4mg/ml) results in an inhibitory effect on cell growth in HT-29 cells. Growth inhibition by Euphorbiae lathyridis Semen extract in HT-29 cells was related with the inhibition of proliferation and induction of apoptosis. The Euphorbiae lathyridis Semen extract induces DNA fragmentation in HT-29 cells. Furthermore, Euphorbiae lathyridis Semen extract induces cell apoptosis through the activation of caspases-3, caspase-9 and PARP cleavage. Conclusion : Euphorbiae lathyridis Semen extract induces apoptosis in human colon cancer cells, therefore, we suggest that Euphorbiae lathyridis Semen extract can be used as a novel class of anti-cancer drugs.

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