The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지

Cytoprotective effects of liquiritigenin, a component of licorice, against lead-induced cytotoxicity in PC-12 cells.

PC-12 cell에서 감초성분의 Liquiritigenin이 납에 의해 유도된 세포독성과 nitric oxide production에 미치는 영향

  • Park, Eun-Young (College of Oriental Medicine, Development Team for The New Drug of Oriental Medicine (BK21 program) Daegu Haany University) ;
  • Park, Sook-Jahr (College of Oriental Medicine, Development Team for The New Drug of Oriental Medicine (BK21 program) Daegu Haany University) ;
  • Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University) ;
  • Jee, Seon-Young (College of Oriental Medicine, Daegu Haany University) ;
  • Byun, Sung-Hui (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Oriental Medicine, Development Team for The New Drug of Oriental Medicine (BK21 program) Daegu Haany University)
  • 박은영 (대구한의대학교 한의과대학, 한방신약개발연구팀(BK21)) ;
  • 박숙자 (대구한의대학교 한의과대학, 한방신약개발연구팀(BK21)) ;
  • 이종록 (대구한의대학교 한의과대학) ;
  • 지선영 (대구한의대학교 한의과대학) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학, 한방신약개발연구팀(BK21))
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Licorice has been commonly used as a detoxification agent. We previously reported that licorice and its component, liquiritigenin, exhibits cytoprotective activity against Pb-induced toxicity. The present study was conducted to evaluate the effect of liquiritigenin on the lead-induced cytotoxicity in PC-12 cells. Methods : PC-12 cells were pre-treated with liquiritigenin, and further incubated with lead 100 ${\mu}M$ for $12^{\sim}48$ hours. The viability of PC-12 cells was measured by MTT assay, and the levels of proteins were analysed by western blot. Results : Severe cytotoxicity was induced and nitric oxide (NO) production was augmented by the exposure of lead. Liquiritigenin protected cells from lead-induced cytoxicity and reduced NO production in a dose-dependent manner. The inhibition of NO production was due to the suppression of iNOS protein via the inhibition of $NF-{\kappa}B$ nuclear translocation, determined by western blot analysis. Conclusions : These results suggest that liquiritigenin may exert cytoprotective effect against lead toxicity by inhibiting NO production.

Keywords