The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Anti-Tumor Metastasis Activity by Extracts of Inonotus obliquus

차가버섯 추출물의 종양 전이 억제 효과

  • 윤택준 (유한대학 식품영양학과) ;
  • 문원국 ((주)내추럴에프엔피) ;
  • 이광호 (건국대학교 의료생명대학 생명공학전공)
  • Published : 2007.09.30
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Abstract

In experimental lung metastasis of colon26-M3.1 carcinoma cells, we found that the prophylactic or therapeutic admini-stration of Inonotus obliquus extracts significantly inhibited lung metastasis. In an in vitro cytotoxicity analysis, the extracts did not affect colon26-M3.1 cell growth at concentrations up to 1000 ${\mu}g/m{\ell}$. Peritoneal macrophages that were stimulated with the extracts produced $TNF-{\alpha}$. These data suggest that Inonotus obliquus extract has antitumor activity to inhibit tumor metastasis, and its antitumor effects are partially associated with macrophages activation.

Keywords

References

  1. Azuma, I and Seya, T. Development of irnrnunoadjuvants for immunotherapy of cancer. Int. Immunopharmcol. 1:1249-1259. 2001 https://doi.org/10.1016/S1567-5769(01)00055-8
  2. Sfondrini, L, Balsari, A and Menard, S. Innate immunity in breast carcinoma. Endocr. Relat. Cancer. 10:301-308. 2003 https://doi.org/10.1677/erc.0.0100301
  3. Murray, HW and Nathan, CF. Macrophage microbicidal mechanisms in vivo: reactive nitrogen versus oxygen intermediates in the killing of intracellular visceral Leishmania donovani. J. Exp. Med. 189:741-746. 1999 https://doi.org/10.1084/jem.189.4.741
  4. Robinson, LA, Tu, L, Steeber DA, Preis, O, Platt, JL and Tedder, TF. The role of adhesion molecules in human leukocyte attachment to porcine vascular endothelium: implications for xenotransplantation. J. Immunol. 161:6931-6938. 1998
  5. Saiki, I, Saito, S, Fujita, C, Ishida, H, lida, J, Murata, J, Hasegawa, A and Azuma, I. Induction of twnoricidal macrophages and production of cytokines by synthetic muramyl dipeptide analogues. Vaccine. 6:238-244. 1988 https://doi.org/10.1016/0264-410X(88)90218-6
  6. Fisher, M and Yang, LX. Anticancer effects and mechanisms of polysaccharide-K (PSK): implications of cancer immunotherapy. Anticancer Res. 22:1737-1754. 2002
  7. Yoon, TJ, Yoo, YC, Hong, EK, Cho, YH, Lee, SW, Azuma, I, Yoo, BI and Kim, JB. Effect of Korean Mistletoe extracts on the induction of IL-I and TNF-alpha from mouse macrophages. Kor. J. Pharmacogn. 25:132-139. 1994
  8. Hwang, SY, Yang, ET, Yeo, JH, Jin, JY, Kim, HS, Park, WB and Sub, JJ. Anti-tumor effect of Korean mistletoe extract intensified with mistletoe lectin against melanoma cells in vitro and in vivo. Kor. J. Pharmacogn. 34:218-222. 2003
  9. Stauder, H and Kreuser, ED. Mistletoe extracts standardised in terms of mistletoe lectins (ML I) in oncology: current state of clinical research. Onkologie. 25:374-380. 2002 https://doi.org/10.1159/000066058
  10. Shin, JY, Song, N, Yun, YS, Yang, HO, Rhee, DK and Phy, S. Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol. Immunotoxicol. 24:469-482. 2002 https://doi.org/10.1081/IPH-120014730
  11. Ha, ES, Hwang, SH, Shin, KS, Yu, KW, Lee, KH, Choi, JS, Park, WM and Yoon, TJ. Anti-metastatic activity of glycoprotein fractionated from Acanthopanax senticosus, involvement of NK-cell and macrophage activation. Arch. Pharm. Res. 27:217-224. 2004 https://doi.org/10.1007/BF02980109
  12. Ohno, N, Furukawa, M, Miura, NN, Adachi, Y, Motoi, M and Yadomae, T. Antitumor beta glucan from the cultured fruit body of Agaricus blazei. Biol. Pharm. Bull. 24:820-828. 2001 https://doi.org/10.1248/bpb.24.820
  13. Saar, M. Fungi in Khanty fork medicine. J. Ethnopharmacol. 31:175-179. 1991 https://doi.org/10.1016/0378-8741(91)90003-V
  14. Ichimura, T, Otake, T, Mori, H and Maruyama, S. HIV-1 protease inhibition and anti-HIV effect of natural and synthetic water-soluble lignin-like substances. Biosci. Biotechnol. Biochem. 63:2202-2204. 1999 https://doi.org/10.1271/bbb.63.2202
  15. Cui, Y, Kim, DS and Park, KC. Antioxidant effect of Inonotus obliquus. J. Ethnopharmacol. 96:79-85. 2005 https://doi.org/10.1016/j.jep.2004.08.037
  16. Fidler, IJ. Macrophage and metastasis-a biological approach to cancer therapy: presidential address. Cancer Res. 45: 4714-4726. 1985
  17. Gerlier, D and Thomasset, N. Use of MTT colorimetric assay to measure cell activation. J. Immunol. Meth. 94:57-63. 1986 https://doi.org/10.1016/0022-1759(86)90215-2
  18. Kudo, C, Saito, M and Yoshida, T. Curative treatments of murine colon26 solid tumors by immunochemotherapy with G-CSF and OK-432. Immunopharm. 29:235-243. 1995 https://doi.org/10.1016/0162-3109(95)00060-7
  19. Shin, JY, Song, JY, Yun, YS, Yang, HO, Rhee, DK and Phy, S. Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol. Immunotoxicol. 24:469-482. 2002
  20. Yoon, TJ, Yoo, YC, Kang, TB, Her, E, Kim, SH, Kim, K, Azuma, I and Kim, JB. Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe(Viscum album colaratum). Int. Immunopharmacol. 1:881-889. 2001 https://doi.org/10.1016/S1567-5769(01)00024-8
  21. Yoon, TJ, Lee, CK, Park, TK and Lee, KH. Immunostimulant and anti-tumor activity of crude extracts of Galium aparine L. Kor. J. Pharmacogn. 36:332-337. 2005
  22. Wynn, TA, Freund, YR and Paulnock, DM. TNF-alpha differentially regulates Ia antigen expression and macrophage tumoricidal activity in two murine macrophage cell lines. Cell Immunol. 140:184-196. 1992 https://doi.org/10.1016/0008-8749(92)90186-S
  23. Holmgren, J, Czerkinsky, C, Eriksson, K and Mharandi, A. Mucosal immunisation and adjuvants: a brief overview of recent advances and challenges. Vaccine 21(Suppl 2):S89-95. 2003 https://doi.org/10.1016/S0264-410X(03)00206-8
  24. Israf, DA, Lajis, NH, Somchit, MN and Sulaiman, MR. Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract. Life Science 75:397-406. 2004 https://doi.org/10.1016/j.lfs.2003.10.038
  25. Sung, JY, Yoon, TJ, Yu, KW, Lee, KH and Lee, H. Enhancement of immunological activities in mice by oral administration of pectic polysaccharides from Eleutherococus senticosus. Food Sci. Biothechnol. 15:117-121. 2006