The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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The Effect of Conjugated Linoleic Acid(CLA) Supplemented to Different Fat Sources on Fat Depositions and Stearoyl-CoA Desaturase l(SCDl) Gene Expression in Mice

지방 급원을 달리한 식이에 첨가된 CLA가 지방조직과 Stearoyl-Co A Desaturase 1(SCD 1) 발현에 미치는 영향

  • Lee, Se-Na (Dept. of Food and Nutrition, College of Natural Sciences, Duksung Women's University) ;
  • Kang, Keum-Jee (Dept. of Food and Nutrition, College of Natural Sciences, Duksung Women's University)
  • 이세나 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 강금지 (덕성여자대학교 자연과학대학 식품영양학과)
  • Published : 2007.09.30
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Abstract

This study investigated the effects of conjugated linoleic acid(CLA) on the fat deposition, triglyceride levels and the expression of stearoyl-CoA desaturase 1(SCD1) in the livers of male ICR mice that were fed with either soybean oil or beef tallow supplemented with CLA. Mice weighing $25{\sim}30$ g were divided into four groups; soybean oil(SBO), and SBO supplemented with 1% CLA(SBOC), beef tallow(BT) and BT supplemented with 1% CLA(BTC). Each group consisted of 10 mice that were fed the experimental diets for 4 weeks. The experimental diets consisted of 64% carbohydrate, 20% protein, and 16% fat in terms of their contributions to total calories. All other nutrients were identical in the diets. Triglyceride measurements were completed using a kit. Fatty acid compositions were analyzed in the liver using gas chromatography. The levels of SCD1 expression were analyzed by RT-PCR in the liver. No significant differences were found for food intake level, body weight and food efficiency among the experimental groups. However, the weights of epididymal fat pads and plasma triglyceride levels were significantly lower in SBOC and BTC(p<0.05) compared to the SBO and BT groups. These effects were similar in the CLA supplemented groups. The expression level of SCD1 gene and ${\Delta}9$ desaturase index were not significantly different, regardless of the fat used for CLA supplementation. Based on these results, addition of CLA showed decreasing effects on the fat depots weight and the concentration of triglyceride regardless of the fat sources. The SCD1 gene expression and ${\Delta}9$ desaturase index were not influenced by the types of fats with respect to the CLA effects.

Keywords

References

  1. Kelly, GS. Conjugated linoleic acid: a review. Altern. Med. Rev. 6:367-382. 2001
  2. Liew, C, Schut, HA, Chin, SF, Pariza, MW and Dashwood, RH. Protection of conjugated linoleic acids against 2-amino 3-methylimiclazo[4,5-f]quinoline-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms. Carcinogenesis 16:3037-3043. 1995 https://doi.org/10.1093/carcin/16.12.3037
  3. Lee, KN, Kritchevsky, D and Pariza, MW. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108: 19-25. 1994 https://doi.org/10.1016/0021-9150(94)90034-5
  4. Cook, ME, Miller, CC, Park, Y and Pariza, M. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poult. Sci. 72: 1301-1305. 1993 https://doi.org/10.3382/ps.0721301
  5. Park, Y, Albright, KJ, Liu, W, Stroskon, JM, Cook, ME and Pariza, MW. Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858. 1997 https://doi.org/10.1007/s11745-997-0109-x
  6. Peters, JM, Park, Y, Bonzlez, FJ and Pariza, MW. Influence of conjugated linoleic acid on body composition and target gene expression in peroxisome proliferator-activated receptor alpha-null mice. Biochim. Biophys. Acta. 1533:233-242. 2001 https://doi.org/10.1016/S1388-1981(01)00155-X
  7. Rahman, SM, Wang, YM, Yotsumoto, H, Cha, J, Han, S, Inoue, S and Yanagita, T. Effects of conjugated linoleic acid on serum leptin concentration, body fat accumulation, and beta-oxidation of fatty acid in OLETF rat. J Nuir. 17:385-390. 2001
  8. De Deckere, EA, Van Amelsvoort, JM, McNeill, GP and Jones, P. Effects of conjugated linoleic acid(CLA) isomers on lipid levels and peroxisome proliperation in the hamster. Br. J Nutr. 82:309-317. 1999
  9. Weigand, BR, Swan, JE, Larsen, ST, Parrish, FC and Baas, TJ. Conjugated linoleic acid improves feed efficiency, decreases backfat and improves pork quality attributes. J Anim. Sci. 79:2187-2195. 2001
  10. Wang, YW and Jones, PJH. Conjugated linoleic acid and obesity control: efficacy and mechanisms. Int. J. Obes. Relat. Metab. Disord. 28:941-955. 2004 https://doi.org/10.1038/sj.ijo.0802641
  11. Hargrave, KM, Meyer, BJ, Li, C, Azain, MJ, Baile, CA and Miner, JL. In:f1uence of dietary conjugated linoleic acid and fat source on body fat and apoptosis in mice. Obesity. Res. 12:1435-1444. 2004 https://doi.org/10.1038/oby.2004.180
  12. Dobrzyn, A and Ntambi, J. The role of stearoyl-Co A desaturase in body weight regulation. Trends Carciovasc. Med. 14:77-81. 2004 https://doi.org/10.1016/j.tcm.2003.12.005
  13. Nakamura, MT and Nara, TY. Structure, function, and dietary regulation of 6, 5, and 9 desaturases. Annu. Rev. 24:345-376. 2004 https://doi.org/10.1146/annurev.nutr.24.121803.063211
  14. Lee, KN, Pariza, MW and Ntambi, JM. Conjugated linoleic acid decrease hepatic stearoyl-Co A desaturase mRNA expression. Biochem. Biophy. Res. 248:817-821. 1998 https://doi.org/10.1006/bbrc.1998.8994
  15. Choi, YJ, Kim, YC, Han, YB, Park, YH, Pariza, MW and Ntambi, JM. Stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. J Nutr. 130: 1920-1924. 2000 https://doi.org/10.1093/jn/130.8.1920
  16. Kang, K, Miyazaki, M, Ntambi, JM and Paliza, MW. Evidence that the anti-obesity effect of conjugated linoleic acid is independent of effects on stearoyl-CoA desaturasel expression and enzyme activity. Biochem. Biophys. Res. Commun. 12:532-537. 2004
  17. Hwang, YH and Kang, KJ. Conjugated linoleic acid reduce adipose depots without reduction of stearoyl coenzyme A desaturase I gene expression. Ann. Nutr. Metab. 52: 126-133. 2007
  18. DeLany, JP and West, DB. Changes in body composition with conjugated linoleic acid. J Am. Coll. Nutr. 19:487s-493s. 2000
  19. Chomczynski, P and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159. 1987
  20. Lepage, G and Roy, CC. Direct trans-esterification of all class of lipid in a one step reaction. J Lipid. Res. 27:114-120. 1986
  21. Park, Y, Storkson, JM, Albright, KJ, Liu, W, and Pariza, MW. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids. 34:235-241. 1999 https://doi.org/10.1007/s11745-999-0358-8
  22. Xu, X, Storkson, J, Kim, S, Sugimoto, K, Park, Y and Pariza, MW. Short-term intake of conjugated linoleic acid inhibits lipoprotein lipase and glucose metabolism but does not enhance lipolysis in mouse adipose tissue. J. Nutr. 133:663-667. 2003
  23. DeLany, JP, Blohm, F, Trutt, AA, Xcimeca, JA and West, DB. Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am. J Physiol. 76:R1172-1179. 1999
  24. West, DB, Delany, JP, Camet, PM, Blohm, F, Truett, AA and Scimeca, J. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am. J. Physiol. 44:R667-672. 1998
  25. Evans, M, Geigerman, C, Cook, J, Curtis, L, Kuebler, B and Mcintosh, M. Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids. 35:899-910. 2000 https://doi.org/10.1007/S11745-000-0599-6
  26. Belury, MA and Kempa-Steczco, A. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32: 199-204. 1997 https://doi.org/10.1007/s11745-997-0025-0
  27. West, DB, Blohm, FY, Truett, AA and DeLany, JP. Conjugated linoleic acid persistently increases total energy expenditure in AKR/J mice without increasing uncoupling protein gene expression. J. Nutr. 130:2471-2477. 2000
  28. Ryder, JW, Portocarrero, CP, Song, XM, Cui, L, Yu, M, Combatsiaris, T, Galuska, D, Bauman, DE, Barbano, DM, Charron, MJ, Zierath, JR and Houseknecht, KL. Isomerspecific antidiabetic properties of conjugated linoleic acid. Improved glucose tolerance, skeletal muscle insulin action, and UCP2 gene expression. Diabetes 50: 1149-1157. 2001 https://doi.org/10.2337/diabetes.50.5.1149
  29. Choi, Y, Kim, YC, Han, YB, Park, Y, Pariza, MW and Ntambi, JM. The trans-10, cis-12 isomer of conjugated linoleic acid down regulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. J. Nutr. 130:1920-1924. 2000
  30. Choi, Y, Park, Y, Pariza, MW and Ntambi, JM. Regulation of stearoyl-CoA desaturase activity by the trans-10, cis-12 isomer of conjugated linoleic acid in HepG2 cells. Biochem. Biophys. Res. Commun. 284:689-693. 2001 https://doi.org/10.1006/bbrc.2001.5036
  31. Demaree, SR, Gilbert, CD, Mersmann, HJ and Smith, SB. Conjugated linoleic acid differentially modifies fatty acid composition in subcellular fractions of muscle and adipose tissue but not adiposity of postweaning pigs. J. Nutr. 132: 3272-3279. 2002