Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Anti-inflammatory, anti-ulcer and hypoglycaemic activities of ethanolic and crude alkaloid extracts of Madhuca indica (Koenig) Gmelin seed cake

  • M, Seshagiri (Department of Biochemistry, Gulbarga University) ;
  • RD, Gaikwad (Department of Biochemistry, Gulbarga University) ;
  • KS, Jyothi (Department of Prosthodontics, S.D.M. College of Dental Sciences) ;
  • S, Ramchandra (Department of Pharmacology, S.C.S College of Pharmacy) ;
  • S, Paramjyothi (Department of Biochemistry, Gulbarga University)
  • Published : 2007.06.30
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Abstract

Madhuca indica has been used ethnomedically in Indian folks. In the present study we have investigated anti-inflammatory, anti-ulcer and hypoglycaemic effect of ethanolic extract (EE) and crude alkaloid extract of Madhuca indica seed cake on albino rats. The study showed that the EE had a significant, dose dependent anti-edematogenic, anti-ulcerogenic and hypoglycaemic activity, whereas the crude alkaloid extract exhibited a significant only. Both the extracts possess dose dependent inhibitory activity on carrageenan-induced edema, inhibiting prostaglandins or mediators involved in prostaglandin synthesis, the second phase of inflammation. The EE was significantly effective in protecting pylorus-ligation-induced gastric ulcers at a higher dose level. The active principle of EE seems to be a selective inhibitor of the COX II (prostaglandin synthesis) without important effect on COX I since, EE exhibited both anti-edematogenic and anti-ulcerogenic effect. The EE was effective in reducing the plasma glucose level in normal albino rats in a dose dependent manner, producing hypoglycaemic effect by stimulating the release of insulin from the ${\beta}-cells$ and/or increasing the uptake of glucose from the plasma.

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References

  1. Achrekar S, Kaklij GS, Pote MS, Kelkar SM. (1991) Hypoglycemic activity of Eugenia jambolana and Ficus benalensis: mechanism of action. In Vivo 5, 143-147
  2. Akhtar MS, Akhtar AH, Khan MA. (1992) Antiulcerogenic effects of Ocimum basilicum extracts, volatile oils and flavonoid glycosides in albino rats. Int. J. Pharmacogn. 30, 97-104
  3. Antonio MA, Souza Birto ARM. (1998) Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnem ulmifolia (Turneraceae). J. Ethnopharmacol. 61, 215-228 https://doi.org/10.1016/S0378-8741(98)00049-X
  4. Bajaj S, Srinivasan BP. (1999) Investigations into the anti-diabetic activity of Azadirachta indica. Indian J. Pharmacol. 31, 138-141
  5. Barry H. (1991) Antioxidant effects a basis for drug selection. Drugs 42, 569
  6. Crunkhon P, Meacock SC. (1971) Mediators of the inflammation induced in the rat paw by carrsageenan. Br. J. Pharmacol. 42, 392-402
  7. Dinesh Chandra. (2001) Analgesic effect of aqueous and alcoholic extracts of Madhuka longifolia (Koeing). Indian J. Pharmacol. 33, 108-111
  8. DiRosa M. (1972) Biological properties of carrageenan. J. Pharm. Pharmacol. 24, 89
  9. DiRosa M, Willoughby DA. (1971) Screen for anti-infalmmatory drugs. J. Pharm. Pharmacol. 23, 297-298
  10. Eisenberg DM, Kessler RC, Foster C, Norlock FE, Calkins FR, Delbanco TL. (1993) Unconventional medicine in the United States. Prevalence, cost and pattern of use. N. Engl. J. Med. 328, 246-252
  11. Fort FL, Miyajima H, Ando T, Suzuki T, Yamamoto M, Hamashema T, Sato S, Kitazaki T, Mahoay MC, Hodgen GD. (1995) Mechanism for species-specific induction of leydig cell tumors in rats by lansoprazole. Fundam. Appl. Toxicol. 26, 191-202
  12. Gamache DA, Povlishock JT, Ellis EF. (1986) Carrageenan-induced brain inflammation. Characterization of the model. J. Neurosurg. 65, 679-685
  13. Garcia-Pastor P, Randazzo A, Gomez Paloma L, Alcaraz MJ, Paya M. (1999) Effects of petrosaspongiolide M, a novel phospholipase A2 inhibitor, on acute and chronic inflammation. J. Pharmacol. Exp. Ther. 289, 166-172
  14. Glick D, Von Redlick D, Levine S, Jones L. (1966) Effect of adrenal stimulation on histamine in the rats stomach. Gastroenterology 51, 18-23
  15. Grossman NI, Konturek SJ. (1974) Inhibition of acid secretion in dog by metiamide, a histamine antagonist acting on $H_2$ receptors. Gastroenterology 66, 517-520
  16. Havu N, Mattassow H, Ekman I, Carlsson E. (1990) Enterochromaffin-like cell carcinoids in the rat gastric mucosa following long term administration of ranitidine. Digestion 45, 189-195
  17. Horn J. (2000) The proton-pump inhibitors: Similarities and differences. Clin. Ther. 22, 266-280
  18. Howden CW, Hunt RH. (1994) A pharmacologic approach to gastrointestinal disorders. In: Peptic ulcers disease, edited by Lewis JH, p. 1-22, Maryland, U.S.A.
  19. Kakei N, Ichinose M, Tsukuda S, Tatematsu M, Tezuka N, Yahagi N, Matsushima M, Miki K, Kurokawa K, Takahashi F, Fukamachi H. (1993) Omperazole, a proton pump inhibitor, reduces the secretion, synthesis and gene expression of pepsinogen in rat stomach. Biochem. Biophys. Res. Commun. 195, 997-1004 https://doi.org/10.1006/bbrc.1993.2143
  20. Kulkarni SK, Mehta AK, Kunchandy J. (1986) Anti-inflammatory actions of clonidine, guanfacine and B-HT 920 against various inflammagen-induced acute paw oedema in rats. Arch. Int. Pharmacodyn. Ther. 279, 324-334
  21. Langtry H.D, Wilde MI. (1998) Omeprazole. A review of its uses in Helicobacter pylori infection, gastro-esophageal reflux diseases and peptic ulcers induced by nonsteroidal anti-inflammatory drugs. Drugs 56, 447-486
  22. Maclenan AH, Wilson DH, Taylor AW. (1996) Prevalence and cost of alternative medicine in Australia. Lancet 347, 569-573 https://doi.org/10.1016/S0140-6736(96)91271-4
  23. Martelli A, Mattlioli F, Mereto E, Brambilla Compart G, Sini D, Bergamaschi R, Brabilla G. (1998) Evaluation of omeprazole genotoxicity in a battery of in vitro and in vivo assays. Toxicol 30, 19-41
  24. Mulky MJ, Gandhi VM. (1977) Mowrah (Madhuca latifolia) seed saponin. Toxicological studies. J. Appl. Chem. Biotechnol. 27, 708-713
  25. Nadkarni AK. (1954) Indian Materia Medica, pp. 181, 3rd ed. Vol. 1, Bombay Popular Book Depot., India
  26. Pari L, Ramakrishnan R, Venkateshwaran S. (2001) Antihyperglycaemic effect of Diamed, a herbal formulation, in experimental diabetes in rats. J. Pharm. Pharmacol. 53, 1139-1143
  27. Pawar RS, Bhutani KK. (2004) Madhucosides. A and B. protobassic acid glycosides from Madhuca indica with inhibitory activity on free radical release from phagocytes. J. Nat. Prod. 67, 668-671 https://doi.org/10.1021/np0301792
  28. Peifer MA, Halter JB, Beacd JC, Portel DJ. (1981) Differential effects of tolbutamide on first and second phase insulin secretion in non insulin dependent diabetes mellitus. J Clin. Endocrinol. Metab. 53, 1256-1262
  29. Peterson WL. (1994) A pharmacologic approach to gastrointestinal disorders In: Helicobacter pylori and ulcer disease: treatment consideration, edited by Lewis JH, p. 93-105, Maryland, U.S.A.
  30. Piper DW, Stiel DD. (1986) Pathogenesis of chronic peptic ulcer, current thinking and clinical implications. Med. Prog. 2, 7-10
  31. Powers RE, Lawton GP, Modlin IM. (1995) Genotoxicity, carcinogenicity and acid suppressing medications. Pharmacology 65, 303-317
  32. Rajanarayana K, Sripal Reddy M, Chaluvadi MR, Krishna DR. (2001) Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian J. Pharmacol. 33, 2-16
  33. Ramadan MF, Sharanabasappa G, Paramjyothi S, Seshagiri M, Moersel JT. (2005) Profile and levels of fatty acids and bioactive constituents in mahua butter from fruit-seeds of buttercup tree [Madhuca longifolia (Koenig)]. Eur. Food Res. Technol. DOl 10.1007/s00217-005-0155-2
  34. Rangachari PK. (1975) Histamine release by gastric stimulants. Nature 53, 53-55 https://doi.org/10.1038/253053a0
  35. Rao ChV, Sairam K, Goel RK. (2000) Experimental evaluation of Bacopa monniera on rat gastric ulceration and secretion. Indian J. Physiol. Pharmacol. 44, 35-41
  36. Rastogi L, Patnaik GK, Dikshit M. (1998) Free radicals and antioxidant status following pylorus ligation induced gastric mucosal injury in rats. Pharmacol. Res. 38, 125-132
  37. Sairam K, Rao ChV, Dora Babu M, Agarwal VK, Gael RK. (2002) Antiulcerogenic activity of methanolic extract of Emblica officinalis. J. Ethanopharmacol. 82, 1-9
  38. Shay H, Komarov SA, Fels SS, Meranze D, Gruestein M, Siplet H. (1945) A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 5, 43-61
  39. Shindkawa M, Yamamoto K, Kawakami J, Sawadu Y, Iga T. (1996) Neurotoxic convulsions induced by histamine $H_2$ receptor antagonists in mice. Toxicol. Appl. Pharmacol. 136, 317-323 https://doi.org/10.1006/taap.1996.0038
  40. Singh B, Gambhir SS, Pandey VB, Joshi VK. (1989) Anti-inflammatory activity of Echinops echinatus. J. Ethnopharmacol. 25, 189-200 https://doi.org/10.1016/0378-8741(89)90021-4
  41. Siddiqui BS, Khan S, Karda MN, Aslam H. (2004) Chemical constituents from the fruits of Madhuca latifolia. Helv. Chim. Acta 87, 1194-1201 https://doi.org/10.1002/hlca.200490109
  42. Szolcsanyi J, Helyes Z, Oroszi G, Nemeth J, Pinter E. (1998) Release of somatostatin and its role in the mediation of the anti-inflammatory effect induced by antidromic stimulation of sensory fibres of rat sciatic nerve. Br. J. Pharmacol. 123, 936-942 https://doi.org/10.1038/sj.bjp.0701685
  43. Thepenier P, Jacquier M, Massiot G, LeMen-olivier L, Delaude C. (1990) Alkaloids from seeds of Strychnos variabilis and Strychnos longicaudata. Phytochemistry 29, 686-687
  44. Trease GE, Evans WC. (1983) Textbook of pharmacognosy, pp. 383, 12th ed, Bailliere Tindall., London
  45. Varier PSV. (1995) Indian Medicinal Plants, Vol. 3, pp. 362, Arya Vaidyasala Kottakkal, Orient Longman
  46. Vigneri R, Pezzino V, Wang KY. (1982) Comparison of the in vitro effect of biguanides and sulfonylureas on the insulin binding of its receptors in target cells. J. Clin. Endocrinol. Metab. 54, 95-100
  47. Wandall JH. (1992) Effect of omeprazole on the neutrophil chemotaxis, superoxide production, degranulation and translocation of cytochrome b-245. Gut 33, 617-621 https://doi.org/10.1136/gut.33.5.617
  48. WHO study group on diabetes mellitus. (1985) Technical report series No. 727, World Health Organization, Geneva
  49. WHO study group on diabetes mellitus (1994) Technical report series No. 844, World Health Organization, Geneva, p. 3, 78-79
  50. Winter CA, Risley EA, Nuss GW. (1962) Carrageenan induced edema in hind paw of rat as assay for anti-inflammatory drugs. Exp. BioI. Med. 111, 544-547
  51. Wolfe MM, Sachs G. (2000) Acid suppression: optimizing therapy for gastroduodenal ulcer healing, gastro-esophageal reflux disease, and stress-related erosive syndrome. Gasteroenterology 118, S9-S31
  52. Yanardag R, Colak H. (1998) Effect of chard (Beta vulgaris L. var. cicla) on blood glucose levels in normal and alloxan-induced diabetic rabbits. Pharm. Pharmacol. Communi. 4, 309-311
  53. Yoshikawa K, Tanaka M, Arihara S, Pal BS, Roy SK, Matsumura E, Katayama S. (2000) New oleanene triterpinoid saponins from Madhuca indica. J. Nat. Prod. 63, 1679-1681 https://doi.org/10.1021/np000351r
  54. Yosiokal I, Inada A, Kitagawa I. (1974) Structures of a genuine sapogenol protobassic acid and a prosapogenol of seed kernels of Madhuca indica. Tetrahedron 30, 707-714 https://doi.org/10.1016/S0040-4020(01)97155-8

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