Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Inhibitory effect of Yongdamsagantang water extract on IL-6 and nitric oxide production in lipopolysaccharide-activated RAW 264.7 cells

  • Lim, Jin-Ho (College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Oriental Medicine, Daegu Haany University, Development Team for The New Drug of Oriental Medicine (BK21 program) Daegu Haany University) ;
  • Jee, Seon-Young (College of Oriental Medicine, Daegu Haany University)
  • Published : 2007.09.30
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Abstract

The present study was conducted to evaluate the effect of Yongdamsagantang (YST) on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in RAW 264.7 cells. After the treatment of YST water extract, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. Inducible nitric oxide synthase (iNOS) and phospholylation of inhibitor of nuclear factor kappa B alpha ($p-I{\kappa}B{\alpha}$) were determined by Immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. Results provided evidences that YST inhibited the production of NO. iNOS, and interleukin-6, and the activation of $p-I{\kappa}B{\alpha}$ in RAW 264.7 cells activated with lipopolysaccharide. These findings showed that YST could have some anti-inflammatory effects which might play a role in therapy in Gram-negative bacterial infections.

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References

  1. Beutler B, Cerami A. (1988) The history, properties, and biological effects of cachectin. Biochemistry 27, 7575-7582 https://doi.org/10.1021/bi00420a001
  2. Beutler B, Cerami A. (1989) The biology of cachectin/ TNF-a primary mediator of the host response. Annu. Rev. Immunol. 7, 625-655 https://doi.org/10.1146/annurev.iy.07.040189.003205
  3. Bogdan C. (2001) Nitric oxide and the immune response. Nat. Immunol. 2, 907-16 https://doi.org/10.1038/ni1001-907
  4. Bolli R, Shinmura K, Tang XL, Kodani E, Xuan YT, Guo Y, Dawn B. (2002) Discovery of a new function of cyclooxygenase (COX)-2: COX-2 is a cardioprotective protein that alleviates ischemia/ reperfusion injury and mediates the late phase of preconditioning. Cardiovasc. Res. 55, 506-519 https://doi.org/10.1016/S0008-6363(02)00414-5
  5. Bravo J, Heath JK. (2000) Receptor recognition by gp130 cytokines. EMBO J. 19, 2399-2411 https://doi.org/10.1093/emboj/19.11.2399
  6. Castranova V. (2004) Role of nitric oxide in the progression of pneumoconiosis. Biochemistry 69, 32-37 https://doi.org/10.1023/B:BIRY.0000016348.34175.53
  7. Chen F, Castranova V, Shi X. (2001) New insight into the role of nuclear factor-kappaB in cell growth regulation. Am. J. Pathol. 159, 387-397
  8. Delgado AV, McManus AT, Chambers JP. (2003) Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides 37, 355-361 https://doi.org/10.1016/j.npep.2003.09.005
  9. Ghosh S, May MJ, Kopp EB. (1998) NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses. Annu. Rev. Immunol. 16, 225-260 https://doi.org/10.1146/annurev.immunol.16.1.225
  10. Goetz FW, Planas JV, MacKenzie S. (2004) Tumor necrosis factors. Dev. Comp. Immunol. 28, 487-497 https://doi.org/10.1016/j.dci.2003.09.008
  11. Hedger MP, Meinhardt A. (2003) Cytokines and the immune-testicular axis. J. Reprod. Immunol. 58, 1-26 https://doi.org/10.1016/S0165-0378(02)00060-8
  12. Hu X, Wang L, Yan S et. al. (1999) Prescriptions of Traditional Chinese Medicine. pp. 126-127, Publishing House of Shanghai College of Traditional Chinese Medicine, Shanghai
  13. Jang SI, Kim HJ, Hwang KM, Bae HO, Yun YK, Jung HT, Kim YC. (2002) Anti-Inflammatory Effect of Ethanol Extract of Angelicauchiyamana in Activated Murine RAW 264.7 macrophages. Korean J. Orient. Med. Prescrition 10, 189-197
  14. Karin M, Ben-Neriah Y. (2000) Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. Annu. Rev. Immunol. 18, 621-663 https://doi.org/10.1146/annurev.immunol.18.1.621
  15. Kawamata H, Ochiai H, Mantani N, Terasawa K. (2000) Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RA W264.7 cells, a murine macrophage cell line. Am. J. Chin. Med. 28, 217-226 https://doi.org/10.1142/S0192415X0000026X
  16. Kubes P. (2000) Inducible nitric oxide synthase; a little bit of good in all of us. Gut 47, 6-9 https://doi.org/10.1136/gut.47.1.6
  17. Lee AK, Sung SH, Kim YC, Kim SG. (2003) Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-$_{\alpha}$ and COX-2 expression by sauchinone effects on l-$_{\kappa}B_{\alpha}$ phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 139, 11-20 https://doi.org/10.1038/sj.bjp.0705231
  18. Lee BG, Kim SH, Zee OP, Lee KR, Lee BY, Han JW, Lee HW. (2000) Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two beta-carboline alkaloids extracted from Melia azedarach. Eu.r J. Pharmacol. 406, 301-309 https://doi.org/10.1016/S0014-2999(00)00680-4
  19. MacMicking J, Xie QW, Nathan C. (1997) Nitric oxide and macrophage function. Ann. Rev. Immunol. 15, 323-350 https://doi.org/10.1146/annurev.immunol.15.1.323
  20. Miljkovic D, Trajkovic V. (2004) Inducible nitric oxide synthase activation by interleukin-17. Cytokine Growth Factor Rev. 15, 21-32 https://doi.org/10.1016/j.cytogfr.2003.10.003
  21. Na H, Cha DS, Jeon S, Bu Y, Jeong W, Jeon H. (2006) Chrysanthemum morifolium inhibits inflammatory responses in IFN-$_{\gamma}$ and LPS-induced mouse peritoneal macrophages. Orient. Pharm. Exp. Med. 6, 161-166
  22. Palmer RM, Ashton DS, Moncada S. (1998) Vascular endothelial cells synthesize nitric oxide from Larginine. Nature 333, 664-666 https://doi.org/10.1038/333664a0
  23. Park HJ, Yoon SW, Yoon YW, Yoon HJ, Ko WS. (2002) Inhibitory effect of Omisodok-eum on the secretion of NO in LPS-stimulated mouse peritoneal macrophages. Korean J Orient. Physiol. Pathol. 16, 921-927
  24. Ravi R, Bedi A. (2004) NF-kappa B in cancer-a friend turned foe. Drug Resist. Updat. 7, 53-67 https://doi.org/10.1016/j.drup.2004.01.003
  25. Sanlioglu AD, Aydin C, Bozcuk H, Terzioglu E, Sanlioglu S. (2004) Fundamental principals of tumor necrosis factor-alpha gene therapy approach and implications for patients with lung carcinoma. Lung Cancer 44, 199-211 https://doi.org/10.1016/j.lungcan.2003.11.017
  26. Senftleben U, Karin M. (2002) The IKK/NF-kappa B pathway. Crit. Care Med. 30, 18-26
  27. Seo WG, Pae HO, Oh GS, Chai KY, Kwon TO, Yun YG, Kim NY, Chung HT. (2001) Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, RAW 264.7 cells. J. Ethnopharmacol. 76, 59-64 https://doi.org/10.1016/S0378-8741(01)00221-5
  28. Seo WG, Pae HO, Oh GS, Chai KY, Yun YG, Kwon TO, Chung HT. (2000) Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide. Gen. Pharmacol. 35, 21-28 https://doi.org/10.1016/S0306-3623(01)00086-6
  29. Shishodia S, Aggarwal BB. (2004) Nuclear factor-kappaB: a friend or a foe in cancer? Biochem. Pharmacol. 68, 1071-1080 https://doi.org/10.1016/j.bcp.2004.04.026
  30. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. (2001) Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat. Res. 1, 480-481, 243-268 https://doi.org/10.1016/S0027-5107(01)00183-X
  31. Surh YJ. (1999) Molecular mechanisms of chemopreventive effects of selected dietary and medicinal Phenolic substances. Mutat. Res. 428, 305-327 https://doi.org/10.1016/S1383-5742(99)00057-5
  32. Surh YJ. (2002) Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem. Toxicol. 40v 1091-1097 https://doi.org/10.1016/S0278-6915(02)00037-6
  33. Vassalli P. (1992) The pathophysiology of tumor necrosis factor. Annu. Rev. Immunol. 10, 411-452 https://doi.org/10.1146/annurev.iy.10.040192.002211
  34. Wu KK. (1996) Cyclooxygenase-2 induction: molecular mechanism and pathophysiologyc roles. J. Lab. Clin. Med. 128, 242-245 https://doi.org/10.1016/S0022-2143(96)90023-2
  35. Yang Yuzhou. (2001) Chinese Medical Formulae. pp. 142-143, People' medical publishing house, Beking
  36. Yoon TG, Byun BH, Kwon TK, Suh SI, Byun SH, Kwon YK, Kim SC. (2004) Inhibitory effect of Farfarae Flos water extract on COX-2, iNOS expression and Nitric Oxide production in lipopolysaccharide - activated RAW 264.7 cells. Korean J. Orient. Physiol. Pathol. 18, 908-913