Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Toxicity Test of Methylcarbamate by Ames, Micronucleus, Comet Assays and Microarray Analysis

  • Kwon, Kyoung-J. (College of Pharmacy, Ewha Womans University) ;
  • Go, Seo-Y. (College of Pharmacy, Ewha Womans University) ;
  • Park, Sue-N. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Sheen, Yhun-Y. (College of Pharmacy, Ewha Womans University)
  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Carbamates have excellent insecticidal activities against a broad spectrum of insects. They possess knocking-down, fast-killing, and systemic effects, however, they are toxic to mammals. In this study, we have carried out in vitro genetic toxicity test of methylcarbamate and microarray analysis of differentially expressed genes in response to methylcarbamate. Methylcarbamate did not show mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. Methylcarbamate did not show mutations in frame shift TA98 both with and without exogenous metabolic activation. Methylcarbamate showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. Methylcarbamate did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to methylcarbamate selected differentially expressed 132 genes that could be candidate biomarkers of genetic toxic action of methylcarbamate.

Keywords

References

  1. Affymetrix, Inc. (2000). $GeneChip^{\circledR}$Expression Analysis Technical Manual http://www.affymetrix.com/support/technical/manual/expression_manual.affx
  2. Cairns J., Bidwell J. R. and Arnegard M. E. (1996). Toxicity testing with communities microcosms, mesocosms, and wholesystem manipulation, Rev. Environ. Contam. Toxicol. 147, 45-69
  3. Campanella L., Cubadda F., Sammartino M. P. and Saoncella A. (2000). An algal biosensor for the monitoring of water toxicity in estuarine environments, Water Res 25, 69-76
  4. Duan X. S., Yang L. Y. and Ye P. (1998). The synthesis of thiodicarb, Pesticides 37, 7-8
  5. Eya B. K. and Fukuto T. R. (1986). Formamidine-S-carbamates: A new procarbamate analogue with improved ovicidal and acaricidal activities, J. Agric. Food. Chem. 34, 947-952 https://doi.org/10.1021/jf00072a004
  6. Fahmy M. A. H., Mallipudi N. M. and Fukuto T. R. (1978). Selective toxicity of N,N°À-thiodicarbamates, J. Agric. Food Chem. 26, 550-557 https://doi.org/10.1021/jf60217a021
  7. Fenech M. (2000). The in vitro micronucleus technique, Mutat Res. 455, 81-95 https://doi.org/10.1016/S0027-5107(00)00065-8
  8. Gatehouse D., Haworth S., Cebula T., Gocke E., Kier L., Matsushima T., Melcion C., Nohmi T., Venitt S. and Zeiger E. (1994). Recommendations for the performance of bacterial mutation assays, Mutat. Res. 312, 217-233 https://doi.org/10.1016/0165-1161(94)90037-X
  9. Kasamatsu T., Ogura R., Ikeda N., Morita O., Saigo K., Watabe H., Saito Y. and Suzuki H. (2005). Genotoxicity studies on dietary diacylglycerol (DAG) oil, Food Chem. Toxicol. 43, 253-260 https://doi.org/10.1016/j.fct.2004.10.001
  10. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate M., Kirchner S., Lorge E., Morita T., Norppa H., Surralles J., Vanhauwaert A. and Wakata A. (2003). Report from the in vitro micronucleus assay working group, Mutat. Res. 540, 153-163 https://doi.org/10.1016/j.mrgentox.2003.07.005
  11. Mass W., Van Hes R., Grosscurt A. C. and Deul D. H. (1981). Benzoylphenylurea insecticides. Chemie der Pflanzenschutzund Schandlings Bekanmpfungsmittel; Wegler, R., Ed.; Springer: Berlin, Germany, 423-469
  12. Post L. C. and Vicent W. R. (1973). A new insecticide inhibits chitin synthesis, Naturwissenschaften 60, 431
  13. Sanchez P. and Tarazona J.V. (2002). Development of a multispecies system for testing reproductive effects on aquatic invertebrates. Experience with Daphnia magna, Chironmus prasinus and Lymnaea peregra, Aquat. Toxicol 60, 249-256 https://doi.org/10.1016/S0166-445X(02)00014-0
  14. Singh N. T., McCoy M. T., Tice R. R. and Schneider E. L. (1988). A simple technique for quantification of low levels of DNA damage in individual cells, Exp. Cell. Res. 175, 184-191 https://doi.org/10.1016/0014-4827(88)90265-0
  15. Van Daalen, J. J., Meltzer J. and Mulder R. (1972). Selective insecticide with a novel mode of action, Naturwissenschaften 59, 312 https://doi.org/10.1007/BF00593360
  16. Van der Brink P. J. and Ter Braak C. J. F. (1999). Principal response curves analysis of time-dependent multivariate responses of biological community to stress, Environ. Toxicol. Chem 18, 138-148 https://doi.org/10.1897/1551-5028(1999)018<0138:PRCAOT>2.3.CO;2
  17. Verdisson M., Couderchet and Vernet G. (2001). Effects of procymidone, fludioxonil and pyrimethanil on two non-target aquatic plants, Chemosphere 44, 467-474 https://doi.org/10.1016/S0045-6535(00)00468-9
  18. Verloop A. and Ferrel C. D. (1977). Benzoylphenyl ureass A new group of larvicides interfering with chitin deposition. Pesticide Chemistry in the 20th Century; Plimmer J. R., Ed.; ACS Symposium Series 37; American Chemical Society: Washington, DC, 237 https://doi.org/10.1021/bk-1977-0037.ch013
  19. Wang W. and Freemark K. (1995). The use of plants for environmental monitoring and assessment, Ecotoxicol. Environ. Safety 30, 289-301 https://doi.org/10.1006/eesa.1995.1033
  20. Ware G.W. (2001). An Introduction to Insecticides. In Radcliff's IPM World Textbook 3rd pp. 7-9. MeistroPro Information Resource, Willoughby Ohio
  21. Wong P.K. (2000). Effects of 2,4-D, glyphosate and paraquat on growth, photosynthesis and chlorophyll-a synthesis of Scenedesmus quadricauda Berb 614, Chemosphere 4, 177-182 https://doi.org/10.1016/0045-6535(75)90097-1
  22. Yi M.Q., Liu H.X., Shi X.Y., Liang P. and Gao X.W. (2006). Inhibitory effect of four carbamate insecticides on acetylcholinesterase of male and female Carassius auratus in vitro, Tox. Pharmacol. 143, 113-116
  23. Zeng X.Z., Duan X.S. and Yang L.Y. (1995). Methods for preparation of carbosulfan, Pesticides 34, 12-13

Cited by

  1. Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma vol.33, pp.2, 2012, https://doi.org/10.1007/s10059-012-2271-8
  2. Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome vol.33, pp.3, 2012, https://doi.org/10.1007/s10059-012-2280-7
  3. A novel, integrated in vitro carcinogenicity test to identify genotoxic and non-genotoxic carcinogens using human lymphoblastoid cells vol.92, pp.2, 2018, https://doi.org/10.1007/s00204-017-2102-y
  4. Scientific opinion on the re‐evaluation of dimethyl dicarbonate (DMDC, E 242) as a food additive vol.13, pp.12, 2007, https://doi.org/10.2903/j.efsa.2015.4319