Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Distribution of Parvalbumin-Immunoreactive Retinal Ganglion Cells in the Greater Horseshoe Bat, Rhinolophus ferrumequinum

한국관박쥐 망막에서 파브알부민 면역반응성 망막신경절세포의 분포 양상

  • Jeon, Young-Ki (Department of Ophthalmic Optics, Kundong University) ;
  • Kim, Tae-Jin (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Eun-Shil (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Joo, Young-Rak (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Jeon, Chang-Jin (Department of Biology, College of Natural Sciences, Kyungpook National University)
  • 전영기 (건동대학교 안경광학과) ;
  • 김태진 (경북대학교 자연과학대학 생물학과) ;
  • 이은실 (경북대학교 자연과학대학 생물학과) ;
  • 주영락 (경북대학교 자연과학대학 생물학과) ;
  • 전창진 (경북대학교 자연과학대학 생물학과)
  • Published : 2007.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Parvalbumin occurs in various types of cells in the retina. We previously reported parvalbumin distribution in the inner nuclear layer of bat retina. In the present study, we identified the parvalbumin-immunoreactive neurons in the ganglion cell layer of the retina of a bat, Rhinolophus ferrumequinum, and investigated the distribution pattern of the labeled neurons. Parvalbumin immunoreactivity was found in numerous cell bodies in the ganglion cell layer. Quantitative analysis showed that these cells had medium to large-sized somas. The soma diameter of the parvalbumin-immunoreactive cells in the ganglion cell layer ranged from 12.35 to 19.12 ${\mu}m$ (n=166). As the fibers in the nerve fiber layer were also stained, the majority of parvalbumin-immunoreactive cells in the ganglion cell layer should be medium to large-sized retinal ganglion cells. The mean nearest neighbor distance of the parvalbumin-immunoreactive cells in the ganglion cell layer of the bat retina ranged from 59.57 to 62.45 ${\mu}m$ and the average regularity index was 2.95 ${\pm}$ 0.3 (n=4). The present results demonstrate that parvalbu-min is expressed in medium to large-sized retinal ganglion cells in bat retina, and they have a well-or-ganized distributional pattern with regular mosaics. These results should be important as they are applicable to a better understanding of the unsolved issue of a bat vision. This data will help to provide fundamental knowledge for the better understanding of the unique behavioral aspects of bat flight maneuverability.

파브알부민(pa π albumin)은 망막의 다양한 세포타입에서 분포하고 있다. 본 연구팀은 이전연구에서 박쥐 망막의 내핵층에서의 파브알부민의 분포를 보고하였다. 현재 연구에서 본 연구팀은 한국관박쥐 (Rhinolophus ferrumequinum) 망막의 신경절세포층에 존재하는 파브알부민을 함유하는 신경세포를 규명하였고, 이들 세포의 분포양상을 조사하였다. 실험 결과,파브알부민의 면역반응성은 신경절세포층의 다수 세포에서 발견되었으며, 이들 세포는 주로 중간형 이상 크기의 세포체를 가지고 있었다. 조사된 세포체의 직경은 12.35 - 19.12 ${\mu}m$ 의 범위를 가지며 (n=166), 신경섬유층의 섬유 역시 염색되는 것으로 보아, 파브알부민을 함유하는 신경절세포는 대부분이 중간형이상 크기의 신경절세포임을 뒷받침하고 있다. NND (nearest neighbor distance) 분석을 통해서 본, 평균 NND는 59.57 에서 62.45 ${\mu}m$ 로 나타났으며, 평균 RI (regularity index) 는 2.95 ${\pm}$ 0.3 (mean${\pm}$s.d., n=4) 으로 계산되었다. 이를 종합해보면, 파브알부민은 한국관박쥐 망막의 신경절세포층에서 중간형이상 크기의 신경절세포에서 주로 발현하고 있으며, 이들은 규칙적인 배열을 가진 채 잘 조직화된 분포양상을 보여주고 있음을 알 수 있었다. 이러한 결과들은, 아직까지 명확하게 규명되어 있지 못한 박쥐의 시각에 대한 이해에 중요하게 적용될 수 있을 것이라고 사료된다.

Keywords

References

  1. Badea, T. C. and J. Nathans. 2004. Quantitative analysis of neuronal morphologies in the mouse retina visualized by using a genetically directed reporter. J. Comp. Neurol. 480, 331-351. https://doi.org/10.1002/cne.20304
  2. Baimbridge, K. G., M. R. Celio and J. H. Rogers. 1992. Calcium-binding proteins in the nervous system. Trends Neurosci. 15, 303-308. https://doi.org/10.1016/0166-2236(92)90081-I
  3. Casini, G., D. W. Rickman and N. C. Brecha, 1995. All amacrine cell population in the rabbit retina: identification by parvalbumin immunoreactivity. J. Comp. Neurol. 356, 132-142. https://doi.org/10.1002/cne.903560109
  4. Coombs, J., D. van der List., G. Y. Wang and L. M. Chalupa. 2006. Morphological properties of mouse retinal ganglion cells. Neuroscience 140, 123-136. https://doi.org/10.1016/j.neuroscience.2006.02.079
  5. Haverkamp, S. and H. Wassle. 2000. Immunocytochemical analysis of the mouse retina. J. Comp. Neurol. 424, 1-23. https://doi.org/10.1002/1096-9861(20000814)424:1<1::AID-CNE1>3.0.CO;2-V
  6. Heizmann, C. W. 1993. Calcium signaling in the brain. Acta Neurobiol. Exp. 53, 15-23.
  7. Heizmann, C. W. and U. Kagi. 1989. Structure and function of parvalbumin. Adv. Exp. Med. Biol. 255, 215-222. https://doi.org/10.1007/978-1-4684-5679-0_23
  8. Heizmann, C. W., J. Rohrenbeck and W. Kamphuis. 1990. Parvalbumin, molecular and functional aspects. Adv. Exp. Med. Biol. 269, 57-66. https://doi.org/10.1007/978-1-4684-5754-4_8
  9. Hutsler, J. J., C. A. White and L. M. Chalupa. 1993. Neuropeptide Y immunoreactivity identifies a group of gamma-type retinal ganglion cells in the cat. J. Comp. Neurol. 336, 468-480. https://doi.org/10.1002/cne.903360311
  10. Jeon, C. J., E. Strettoi and R. H. Masland. 1998. The major cell populations of the mouse retina. J. Neurosci. 18, 8936-8946. https://doi.org/10.1523/JNEUROSCI.18-21-08936.1998
  11. Jeon, M. H. and C. J. Jeon. 1998. Immunocytochemical localization of calretinin containing neurons in retina from rabbit, cat, and dog. Neurosci. Res. 32, 75-84. https://doi.org/10.1016/S0168-0102(98)00070-4
  12. Jeon, Y. K., S. Y. Kim and C. J. Jeon. 2001. Morphology of calretinin and tyrosine hydroxylase-immunoreactive neurons in the pig retina. Mol. Cells 11, 250-256.
  13. Jeon, Y. K., T. J. Kim., J. Y. Lee., J. S. Choi and C. J. Jeon. 2007. All amacrine cells in the inner nuclear layer of bat retina: identification by parvalbumin immunoreactivity. Neuroreport 16, 1095-1099.
  14. Jones, G. and J. M. V. Rayner. 1989. Foraging behavior and echolocation of wild horseshoe bats Rhinolophus Jerrumequinum and R. hipposideros (Chiroptera, Rhinolophidae). Behav. Ecol. Sociobiol. 25, 183-191. https://doi.org/10.1007/BF00302917
  15. Kim, T. J. and C. J. Jeon. 2006. Morphological classification of parvalbumin-containing retinal ganglion cells in mouse: single-cell injection after immunocytochemistry. Invest. Ophthalmol. Vis. Sci. 47, 2757-2764. https://doi.org/10.1167/iovs.05-1442
  16. Kong, J. H., D. R. Fish., R. L. Rockhill and R. H. Masland. 2005. Diversity of ganglion cells in the mouse retina: Unsupervised morphological classification and its limits. J. Comp. Neurol. 489, 293-310. https://doi.org/10.1002/cne.20631
  17. Kwon, O. J., J. Y. Kim., S. Y. Kim and C. J. Jeon, 2005. Alterations in the localization of calbindin D28K-, calretinin-, and parvalbumin-immunoreactive neurons of rabbit retinal ganglion cell layer from ischemia and reperfusion. Mol. Cells 19, 382-390.
  18. Ransome, R. D. 1968. The distribution of the Greater horseshoe bat, Rhinolophus ferrumequinum, during hibernation, in relation to environmental factors. J. Zool. 154, 77-112. https://doi.org/10.1111/j.1469-7998.1968.tb05040.x
  19. Ransome, R. D. and A. M. Hutson. 2000. Action plan for the conservation of the greater horseshoe bat in Europe (Rhinolophus ferrumequinum), Nature and Environment, 109, 7-52.
  20. Rockhill, R. L., F. J. Daly., M. A. MacNeil., S. P. Brown and R. H. Masland. 2002. The diversity of ganglion cells in mammalian retina. J. Neurosci. 22, 3831-3843. https://doi.org/10.1523/JNEUROSCI.22-09-03831.2002
  21. Sanna, P. P., K. T. Keyser., M. R. Celio., H. J. Karten and F. E. Bloom. 1993. Distribution of parvalbumin immunoreactivity in the vertebrate retina. Brain Res. 600, 141-150. https://doi.org/10.1016/0006-8993(93)90412-G
  22. Sun, W., N. Li and S. He. 2002. Large-scale morphological survey of mouse retinal ganglion cells. J. Comp. Neurol. 451, 115-126. https://doi.org/10.1002/cne.10323
  23. Sun, W., N. Li and S. He. 2002. Large-scale morphological survey of rat retinal ganglion cells. Vis. Neurosci. 19, 483-493. https://doi.org/10.1017/S0952523802194107
  24. Wassle, H., U. Grunert and J. Rohrenbeck. 1993. Immunocytochemical staining of All-amacrine cells in the rat retina with antibodies against parvalbumin. J. Comp. Neurol. 332, 407-420. https://doi.org/10.1002/cne.903320403
  25. Wassle, H. and H. J. Riemann. 1978. The mosaic of nerve cells in the mammalian retina. Proc. R. Soc. Land. B. Biol. Sci. 200, 441-461. https://doi.org/10.1098/rspb.1978.0026
  26. White, C. A. and L. M. Chalupa. 1991. Subgroup of alpha ganglion cells in the adult cat retina is immunoreactive for somatostatin. J. Comp. Neurol. 304, 1-13. https://doi.org/10.1002/cne.903040102

Cited by

  1. Histochemical Analysis of the Cone cells in the Retina of the Greater Horseshoe Bat Rhinolophus ferrumequinum vol.18, pp.2, 2013, https://doi.org/10.14479/jkoos.2013.18.2.187
  2. Localization of the Major Retinal Neurotransmitters and Receptors and Müller Glia in the Retina of the Greater Horseshoe Bat (Rhinolophus ferrumequinum) vol.20, pp.3, 2015, https://doi.org/10.14479/jkoos.2015.20.3.391
  3. Distribution of Glutamate Receptors in the Retina of the Greater Horseshoe Bat (Rhinolophus ferrumequinum) vol.19, pp.3, 2014, https://doi.org/10.14479/jkoos.2014.19.3.413