Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-diabetic Effect of Crude Polysaccharides from Grifola frondosa in $KK-A^{y}$ Diabetic Mouse and 3T3-L1 Adipocyte

3T3-L1지방세포 및 제2형 당뇨모델($KK-A^{y}$)에서 잎새버섯(Grifola frondosa) 조다당체 추출물의 항당뇨 효과

  • Park, Kum-Ju (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.) ;
  • Oh, Young-Joo (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.) ;
  • Lee, Sang-Yun (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.) ;
  • Kim, Hyun-Su (Division of Life Sciences, Kyungnam University) ;
  • Ha, Hyo-Cheol (Institute of Food & Culture R&D Center for Functional Food, Pulmuone Co., Ltd.)
  • 박금주 (풀무원 식문화연구원 기능성연구소) ;
  • 오영주 (풀무원 식문화연구원 기능성연구소) ;
  • 이상윤 (풀무원 식문화연구원 기능성연구소) ;
  • 김현수 (경남대학교 생명과학부) ;
  • 하효철 (풀무원 식문화연구원 기능성연구소)
  • Published : 2007.06.30
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Abstract

This study was performed to investigate the anti-diabetic mechanism of crude polysaccharides isolated from the fruiting bodies of Grifola frondosa. We treated 3T3-L1 adipocyte cells to observe whether the crude polysaccharides isolated from Grifola frondosa would stimulate insulin sensitivity. Significant insulin sensitizing activity was observed in the 3T3-L1 adipocytes, and giving the crude polysaccharide of Grifola frondosa with 1 nM of insulin caused glucose uptake to increase to a similar level as giving 50 nM of insulin alone. To confirm the mechanism for the anti-diabetic effect of the crude polysaccharides, we performed further examinations within $KK-A^{y}$ mice, an animal model of type 2 diabetes. The crude polysaccharides reduced blood glucose levels in the $KK-A^{y}$ mice for 2 weeks after feeding, and also significantly lowered plasma insulin levels. These results suggest that the anti-diabetic mechanism of the crude polysaccharide of Grifola frondosa is related to the enhancement of insulin sensitivity.

기존의 연구에서 보고된 잎새버섯의 항당뇨 효과에 대한 메카니즘을 설명하고자 본 연구에서는 잎새버섯의 인슐린 민감성 향상 제재로서의 가능성을 확인하고자 하였다. 이를 위하여 잎새버섯 열수추출물의 분획물을 분리하여 인슐린 저항성을 가진 3T3-L1 지방세포에서의 포도당 흡수 정도를 관찰하였다. 실험결과 HW-I이 3T3-L1 지방세포의 인슐린 민감성을 향상시켜 세포 내로의 포도당 섭취를 대조군 대비 4.2배 향상시켰다. 제2형 당뇨모델인 5주령 $KK/A^{y}$ 마우스를 3주간 적응시킨 뒤 HW-I을 8주간 자유 식이 하면서 식이 섭취량, 음수 섭취량 및 체중변화를 관찰하였고 혈액중 포도당 농도, 혈장 인슐린 농도, 당화혈색소를 측정하였으며 결과는 다음과 같다. HW-I식이군은 control군과 비교하여 식이 섭취량 및 최종 체중간에는 유의적인 차이가 없었으나 음수 섭취량에 있어서는 control군에 비해 거의 2배정도 적은 섭취량을 보여 유의적인 차이를 나타내었다(p<0.001). 혈중 포도당 농도는 HW-I 식이군의 경우 control군과 비교하여 식이 1주째 $335.4{\pm}51.8$ mg/dL로 유의적으로 낮은 혈당치를 나타내기 시작하여(p<0.01) 식이 8주째 $311{\pm}96.8$ mg/dL로 유의적인 혈당 억제 효과를 나타내었다(p<0.001). 혈장 인슐린 농도는 HW-I 식이군의 경우 $84.7{\pm}18.9$ ng/mL로 control군의 $342.7{\pm}125$ ng/mL 비교하여 24.7% 수준으로 유의적인 감소 효과를 나타내었다(p<0.001). 당화혈색소 농도는 HW-I 식이군의 경우 8.99%로 control군의 9.87%보다 유의적인 감소 효과를 나타내었다(p<0.05). 이상에서 잎새버섯 열수추출물의 조다당체인 HW-I이 인슐린 민감성 효과가 있는 것으로 밝혀졌으며 제2형 당뇨모델인 $KK-A^{y}$ 마우스를 이용한 동물실험에서도 뚜렷한 혈당강하효과를 나타내어 인슐린 민감성 제재로 개발 할 수 있는 가능성을 보여 주었다.

Keywords

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