생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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약용식물의 항생제 내성균주에 대한 항균활성

Antimicrobial Effect of Medicinal Plants against Methicillin-Resistant Staphylococcus aureus (MRSA)

  • 지영주 (계명대학교 식품가공학) ;
  • 이지원 (계명대학교 식품가공학) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터)
  • Ji, Young-Ju (Department of Food Science and Technology, Keimyung University) ;
  • Lee, Ji-Won (Department of Food Science and Technology, Keimyung University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 발행 : 2007.03.30
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초록

본 연구는 병원 내 감염의 주된 원인균으로 알려진 황색 포도상구균(Staphylococcus aureus : S. aureus)와 항생제 내성구균 (Methicillin-Resistant Staphylococcus aureus : MRSA)에 대해 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어 온 38종의 약용식물을 메탄올에 추출하여 항균활성을 조사해 보았다. MRSA에 대해 항균력 검색을 한 결과 세신, 황련, 호장근, 미역취씨 순으로 높은 항균활성이 나타났다. MRSA로 선별된 4종의 약용식물에 대해 농도와 배지를 다르게 하여 항균력을 측정한 결과 완전 배지인 TSA 배지에서 항균력을 측정한 결과 세신이 고농도인 5 mg/disc에서 S. aureus, MRSA 에 대해각각 21 mm, 19 mm로 우수한 항균력을 보였고, 미역취씨를제외한 3종의 약용식물은 S. aureus에서 MRSA보다 높은 항균력을 보였으며 미역취씨의 경우 두 균주 모두에서 효과가 나타나지 않았다. MSA 배지에서는 4종의 약용식물 모두 TSA 배지보다 높은 저해율을 보였고 특히 세신이 5 mg/disc 농도에서 S. aureus을 30 mm에 가까운 아주 우수한 항균력을 보였으며, 낮은 농도에서도 20 mm이상의 저해를 보였다. 미역취씨의 경우 TSA 배지에서는 항균력을 나타내지 않았으나 MSA배지의 경우 2.5 mg/disc의 낮은 농도에서도 두 균주 모두에서 10 mm이상을 저해하는 항균력을 보였다. 또한 4종의 약용식물을 서로 혼합하였을 경우 항균효과를 측정한 결과 TSA배지의 경우 두 균주 모두에서 세신과 황련을 혼합하였을 경우 세신, 황련의 각각 2.5 mg/disc의 항균력 보다 높게 나타났으며, MSA 배지의 경우 두 균주 모두에서 세신과 혼합한 3종의 약용식물에서 모두 항균력이 상승했고, 특히 호장근과 미역취씨는 20 mm의 높은 항균력을 보였다.

In the present study, we investigated antimicrobial activity of the medicinal plants against various strains of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (S. aureus). Among the tested, the plants extracts of Asiasarum heterotropoides var. mandshuricum, Coptidis rhizoma, Reynoutria elliptica Migo., Solidago virga-aurea var. gigantea Miq.seed exhibited significant antimicrobial activities against MRSA KCCM 11812, 40510 and S. aureus ATCC 25923. The methanol extract of Asiasarum heterotropoides var. mandshuricum showed strong antimicrobial activity against MRSA KCCM 11812, 40510 and S. aureus ATCC 25923 at the 5 mg/disc. A synergistic effect was found in combined extracts of Asiasarum heterotropoides var. mandshuricum and Coptidis rhizoma as compared to each extracts alone. The result suggests that medicinal plant extracts can be used as an effective natural antimicrobial agent in food.

키워드

참고문헌

  1. Ahn, D. K. 2000. Handbook of Korean medical herbs. Kyohak. 440
  2. An, D. K. 2000. A Pictorial Book of the Korean Flora. Kyohak Publishing Co. Ltd. Seoul. 1239
  3. Bae, J. H. 2004. Antimicrobial effect of pulsatilla Koreana extracts on food-borne pathogens. The Korean Nutrition Society 37, 655-661
  4. Bae, J. H. 2005. Antimicrobial effect of plagiorhegama dubium extracts on food-borne pathogen. Korean J. Food & Nutr. 18, 81-87
  5. Bauer, A. W., M. Kirby, J. C. Sherris and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493
  6. Cho, J. Y., I. Choi and E. K. Hwang. 2003. Antimicrobial activity of extracts from medicinal herbs against Escherichia coli. Korea J. Vet. Res. 43, 625-631
  7. Choi, M. Y., E. J. Choi, E. Lee, T. J. Rhim, B. C. Cha and H. J. Park. 1997. Antimicrobial activities of pine needle (Pinus densiflora Seib et Zucc.) extract. Korean J. Appl. Microbial. Biotechnol. 25, 293-297
  8. Choi, O. K., Y. C. Noh and S. Y. Hwang. 2000. Antimicrobial activity of grapefruit seed extracts and polylysine mixture against food-borne pathogens. Korean J. Dietary Culture. 15, 9-15
  9. Chung, G. S. and R. B. Tak 1993. Effects of pH, temperature and food additives on staphylococcal growth and enterotoxin production. Korean J. Vet. Publ. Hlth. 17:13-38
  10. Cohen, S. H., M. M. Morita and M. Bradford. 1991. A-seven year experience with methicillin-resistant Staphylococcus aureus. Am. J. Med. 91, 233S-237S https://doi.org/10.1016/0002-9343(91)90121-D
  11. Goswami, A., R. N. Barua, B. P. Sharma, J. N. Baruah and I. P. Kulanthaive. 1984. Clerodanes from Solidago virga-aurea. Phytochemistry. 23, 837-841 https://doi.org/10.1016/S0031-9422(00)85038-4
  12. Hattori. T., K. Furuta, T. Nagao, T. Nagamatsu, T. Ito and Y. Suzuki. 1992. Studies on the antinephritic effect of plant components(4) : Reduction of protein excretion by berberine and Coptisine in rats with original-type anti-GMB nephritis. Jpn. J. Pharmacol. 59, 156-169
  13. Hishimoto, K., T. Yanagisawa, Y. Okui, Y. Ikeya, M. (Chin) Maruno and T. Fujita. 1994. Studies on anti-allergic components in the roots of Asiasarum sieboldi. Planta Med. 60, 124-127 https://doi.org/10.1055/s-2006-959432
  14. Jayatilake, G. S., H. Jayasuriya, E. S. Lee, N. M. Koonchanok, R. L. Geahlen, C. L. Ashendel, J. L. McLaughlin and C. J. Chang. 1993. Kinase inhibitors from Polygonum cuspidatum. J. Nat. Prod. 56, 1805-1810 https://doi.org/10.1021/np50100a021
  15. Jung, Y. S, K. W. Lee and S. Y. Lee. 1988. Textbook of Diagnostic Microbiology. Danheung. 105-122
  16. Kang, H. J., H. G. Choe and W. G. Son. 1990. Incidence and characterization of coagulase-positive Staphylococcus in bulk milk from dairy farms. Korean J. Vet. Publ. Hlth. 14, 9-13
  17. Kang, H. M., J. S. Moon, G. C. Jang, J. M. Kim, M. D. Song and S. Y. Yang. 2005. Antibacterial effect of Terminaliae chebula extract against major pathogens and methicillin-resistant Staphylococcus aureus (MRSA) from bovine mastitis milk. Korean J. Vet. Res. 45, 113-119
  18. Kang, M. W. and Y. R. Kim. 1993. Infection of Methicillin resistant Staphylococcus aureus. J. Korean Soc. Chemother. 11, 17-26
  19. Kang, S. K., D. Kim and S. K. Park. 1995. Effects of antimicrobial of Leaf Mustard (Brassica juncea) extract on compositions and leakage of cellular materials in Escherichia coli and Staphylococcus aureus. J. Korean Soc. Food Nutr. 24, 280-285
  20. Kang, Y. S., S. K. Yoon. S. H. Jwa, D. H. Lee, G. J. Woo, Y. S. Park and C. M. Kim. 2002. Prevalence of Staphylococcus aureus in Kimbap. J. Food Hyg. Safety. 17, 31-35
  21. Kwak, Y. S., J. W. Yang and K. S. Lee. 1993. Screening of herb drugs showing antimicrobial activity against some pathogenic microorganisms. Kor. J. Food Hygiene. 8, 141-145
  22. Kim, S. G., T. J. Lee, T. Y. Lee and H. S. Kim. 1993. Identification and antimicrobial susceptibility aspects of pathogenic Staphylococcus aureus. J. Korean Soc. Microbiol. 28, 251-259
  23. Kim, S. J. 1995. Screening and characteristics of antimicrobial activity in oriental medicines. Kyungsan National Univ PhD' Dissertation
  24. Kim, T. J. 1997. Wild flowers of korea. Kugil media, Seoul, Korea
  25. Lee, B. W. and D. H. Shin. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Korean J. Food Sci. Technol. 23, 200-204
  26. Lee, H. M., G. Y. Lee, E. K. Yoon, H. J. Kim, Y. S. Kang, D. H. Lee, J. S. Park, S. H. Lee, G. J. Woo, S. H. Kang, J. S. Yang and K. H. Yang. 2004. Computation of maximum edible time using monitoring Data of Staphylococcus aureus in kimbap and micromodel. J. Food Hyg. Safety. 19, 49-54
  27. Mok, S. J., U. Y. Park, Y. M. Kim and D. S. Chang. 1997. Effects of solvents and extracting condition on the antimicrobial activity of salviae miltiorrhizae radix (salvia miltiorrhiza) extract. J. Korean Soc. Food Nutr. 25, 224-228
  28. Park, U. Y., D. S. Chang and H. R. Cho. 1992. Antimicrobial effect of lithospermi radix (lithospermum erythrorhizon) extract. J. Korean Soc. Food Nur. 21, 97
  29. Park, U. Y., D. S. Chang and H. R. Cho. 1992. Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr. 21, 91-96
  30. Sung, J. H., J. O. Lee, J. K. Son, N. S. Park, M. R. Kim, J. G. Kim and D. C. Moon. 1999. Cytotoxic constituents from Solidago virga-aurea var. gigantea Miq. Arch. Pharm. Res. 22, 633-637 https://doi.org/10.1007/BF02975338
  31. Vastano, B. C., Y. Chen, N. Zhu, C. Ho, Z. Zhu and R. T. Rosen. 2000. Isolation and identification of stilbenes in two varieties of Polygonum cuspidatum. J. Agric. Food Chem. 48, 253-256 https://doi.org/10.1021/jf9909196
  32. Yamahara, J. 1976. Central depressive action of coptidis rnizoma and its constituents. Nipp. Yaku. Zas. 72, 899-908 https://doi.org/10.1254/fpj.72.899
  33. Yang, M. S., Y. L. Ha, S. H. Nam, S. U. Choi and D. S. Jang. 1995. Screening of domestic plants with antibacterial activity. Agri. Chem. Biotechb. 38, 584-589
  34. Yeo, S. G., C. W. Ahn, I. S. Kim, Y. B. Park, Y. H. Park and S. B. Kim. 1995. Antimicrobial effect of tea extracts from green tea, oolong tea and black tea. J. Korean Soc. Food Nutr. 24, 293-298
  35. You, Y. O., K. J. Kim, B. M. Min and C. P. Chung. 1999. Staphylococcus iugdunensis - a potential pathogen in oral infection. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics. 88, 297-302 https://doi.org/10.1016/S1079-2104(99)70031-4

피인용 문헌

  1. The effect of Coptidis rhizoma, Chinese Galls and Phytoncide in Malassezia furfur vol.25, pp.7, 2015, https://doi.org/10.5352/JLS.2015.25.7.748