Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of Diesel Exhaust Particulates and Particulate Matters on the ICAM-1 and VCAM-1 Expression in the Lung of Asthma-incuced Mouse

디젤분진 및 미세분진이 천식마우스의 폐조직에서 ICAM-1과 VCAM-1의 발현에 미치는 효과

  • Published : 2007.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This research investigated whether exposure of diesel exhaust particulate (DEP) and particulate metter (PM) effect on intercellular. adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in asthma-induced Balb/c and IL-10 knock out (KO) mouse. Mouse was sensitized with intraperitoneal injection with ovalbumin, followed by challenges with intranasal ovalbumin. After induction of asthma mouse placed in the inhalation chamber and exposed to DEP and PM (10 $mg/m^3$). The evidences of pulmonary inflammation were assessed by immunohistochemical stain and westen blot against ICAM-1 and VCAM-1 in the lung tissue. In the immunohistochemical stain, positive reactions for ICAM-1 and VCAM-1 were much stronger in asthma-induced groups and asthma-induced group with DEP or PM than control groups. Although mild positive reactions were appeared in asthma-induced IL-10 KO mice groups, positive reactions were very strong in the asthma-induced group with DEP or PM. In Western blot, expression of VCAM-1 was increased in asthma-induced group with DEP or PM than asthma-induced groups. In the IL-10 KO mouse, ICAM-1 and VCAM-1 expression were increased in asthma-induced group with DEP or PM than asthma-induced groups. DEP and PM exposure have additive effects on the aggravation of inflammatory signs in the asthma-induced murine model. These results suggest that inhalation of DEP and PM in asthmatic patients may aggravate clinical symptoms.

천식이 유발된 Balb/c마우스와 동일한 조건의 IL-10 KO 마우스에 천식의 원인으로 알려진 DEP와 지하철역내에서 채집한 PM (10 ${\mu}g/m^3$)을 inhalation chamber,에 넣고 하루 4시간씩 흡입시킨 후 시료들을 채취하여 ICAM-1, VCAM-1의발현을 살펴 천식증상의 악화에 DEP와 PM이 어데한 영향을 미치는지 확인하였다. 본 실험의 결과 천식이 유발된 일반 Balb/c 마우스에 있어서는 DEP와 PM의 노출에 의하여 ICAM-1 및 VCAM-1의 발현이 세기관지 주위 조직들에서 미약하게 증가하였다. 그러나 IL-10 KO 마우스의 경우 DEP와 PM을 노출시켰을 때 ICAM-1 및 VCAM-1의 발현이 아주 강하게 증가하였다. 따라서, 본 결과는 IL-10에 대한 항체요법이 천식증상의 완화에 쓰일 수 있는 가능성을 암시하며, 한편 자동차 배기가스와 지하철 미세분진의 발생을 예방할 경우 천식과 관련한 세기관지의 염증을 완화시킬 수 있음을 간접적으로 증명한 것이라 할 수 있다.

Keywords

References

  1. Albelda, S. M., C. W. Smith and P. Ward. 1994. Adhesion molecules and inflammatory injury. FASEB J. 8, 504-512 https://doi.org/10.1096/fasebj.8.8.8181668
  2. Alon, R., P. D. Kassner., M. Woldemar., E. B. Finger., M. E. Hemler and T. A. Springer. 1995. The integrin VLA-4 supports tethering and rolling in flow on VCAM-1. J. Cell. BioI. 128, 1243-1256 https://doi.org/10.1083/jcb.128.6.1243
  3. Boulet, L. P., H. Turcotte., M. Laviolette., F. Naud., M. C. Bernier., S. Martel and J. Chakir. 2000. Airway hyper-responsiveness, inflammation, and subepithelial collagen deposition in recently diagnosed versus long-standing mild asthma. Influence of inhaled corticosteroids. Am. J. Respir. Crit. Care. Med. 162, 1308-1313 https://doi.org/10.1164/ajrccm.162.4.9910051
  4. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  5. Bunn, W. B. III., P. A. Valberg., T. J. Slavin and C. A. Lapin. 2002. What is new in diesel. Int. Arch. Occup. Environ. Health. 75, 122-132 https://doi.org/10.1007/s00420-002-0342-4
  6. Busse, W. Wand R F. Jr. Lemanske. 2001. Asthma. N. Engl. J. Med. 344, 350-362 https://doi.org/10.1056/NEJM200102013440507
  7. Canonica G. W., G. Ciprandi., G. P. Pesce., S. Buscaglia, F. Paolieri and M. Bagnasco. 1995. ICAM-1 on epithelial cells in allergic subjects: A hallmark of allergic inflammation. Int. Arch. Allergy. Immunol. 107, 99-102 https://doi.org/10.1159/000236943
  8. Christer, J., L. Dora., G. Maria., H. B. Eythor., H. Lena and V. Per. 2005. Circulating adhesion molecules in allergic and non-allergic asthma. Respir. Med. 99, 45-51 https://doi.org/10.1016/j.rmed.2004.05.007
  9. Ichinose, T., A. Furutama and M. Sagai. 1995. Biological effects of diesel exhaust particles (DEP) II. Acute toxicity of DEP introduced into lung by intratracheal instillation. Toxicology 99, 153-167 https://doi.org/10.1016/0300-483X(94)03013-R
  10. Jones, D. A., L. V. Melntyre., C. W. Smith and L. J. Picker. 1994. A two-step adhesion cascade fur T cell/endothelial cell interactions under flow conditions. J. Clin. Invest. 94, 2443-2456 https://doi.org/10.1172/JCI117612
  11. Kim, S. C. and S. H. Byun. 2005. The effects of old-enlandiae diffusae herba extract on eosinophil, IgE and IL-4 in experimental asthma induced by ovalbumin. Kor. J. Herbology 20, 35-42
  12. Lein, R. R. and C. Wegner. 1992. Role of intercellular adhesion molecule-1 in the inflammatory response. Kidney Int. 41, 617-619 https://doi.org/10.1038/ki.1992.94
  13. Peterson, B. 1996. Saxon. Global increases in allergic respiratory disease: the possible role of diesel exhaust particles. Ann. Allergy Asthma Immunol. 77, 263-270 https://doi.org/10.1016/S1081-1206(10)63318-2
  14. Pope, C. A. III., D. V. Bates and M. E. Raizenne. 1995. Health effects of particulate air pollution: time for reassessment? Environ. Health Perspect. 103, 472-480 https://doi.org/10.2307/3432586
  15. Ruoslahti, E. 1991. Integrins. J. Clin. Invest. 87, 1-5 https://doi.org/10.1172/JCI114957
  16. Salma, I., M. Willy., Z. P. Eva and A. Gyula. 2001. Comprehensive characteri sation of atmospheric aerosols in Budapest, Hungary: physicochemical properties of inorganic species. Atmos. Environ. 35, 4367-4378 https://doi.org/10.1016/S1352-2310(01)00204-7
  17. Schwartz, J. and D. W. Dockery. 1992. Increased mortality in Philadelphia associated with daily air pollution concentration. Am. Rev. Respir. Dis. 145, 600-604 https://doi.org/10.1164/ajrccm/145.3.600
  18. Schwartz, J., D. W. Dockery and L. M. Neas. 1996. Is daily mortality associated specifically with fine particles? J. Air. Waste. Manag. Assoc. 46, 927-939 https://doi.org/10.1080/10473289.1996.10467528
  19. Smith, C. H., J. Barker and T. H. Lee. 1993. Adhesion molecules in allergic inflammation. Am. Rev. Respir. Dis. 148, 75-78 https://doi.org/10.1164/ajrccm/148.1.75
  20. Springer, T. A. 1990. Adhesion receptors of the immune system. Nature 346, 425-434 https://doi.org/10.1038/346425a0
  21. Springer, T. A. 1994. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301-314 https://doi.org/10.1016/0092-8674(94)90337-9
  22. Wong, C. K., C. B. Wang., M. L. Li, W. K. Ip., Y. P. Tian and C. W. Lam. 2006. Induction of adhesion molecules upon the interaction between eosinophils and bronchial epithelial cell: involvement of p38 MAPK and NF-kappaB. Int. lmmunopharmacol. 6, 1859-1871 https://doi.org/10.1016/j.intimp.2006.08.003