Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The expression of human Spt16 is associated with cell proliferation

인간 Spt16 단백질 발현과 세포 증식 사이의 연관성에 관한 연구

  • Gwak, Jung-Sug (PharmcoGenomics Research Center, Inje University) ;
  • Cho, Mun-Ju (PharmcoGenomics Research Center, Inje University) ;
  • Ryu, Min-Jung (PharmcoGenomics Research Center, Inje University) ;
  • Oh, Sang-Taek (PharmcoGenomics Research Center, Inje University)
  • 곽정숙 (인제대학교 약물유전체연구센터) ;
  • 조문주 (인제대학교 약물유전체연구센터) ;
  • 류민정 (인제대학교 약물유전체연구센터) ;
  • 오상택 (인제대학교 약물유전체연구센터)
  • Published : 2007.03.30
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Abstract

Facilitates chromatin transcription (FACT) is a chromatin-specific elongation factor required for transcription of chromatin templates in vivo and in vitro. FACT consists of human homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein (hSpt16) and the high mobility group-1-like protein structure-specific recognition protein-1 (SSRP-1). Here we show that the protein level of hSpt16 is massively down-regulated in quiescent T98C cells using both immunofluorescence and western blot analysis. In contrast, we observe high level of the hspt16 expression in the proliferative T98G cells. Interestingly, the expression of SSRP-1 is not altered in both quiescent and proliferative states. Taken together, our findings implicate that the expression of hSpt16 is associated with the proliferative state and can be used as a proliferation marker.

FACT(facilitates chromatin transcription)은 크로마틴을 주형으로 하는 전사에 필요한 크로마틴 특이적 전사 진행 인자이다. FACT는 Saccharomyces Cerevisiae Spt16/Cdc68의인간 유사체와 high mobility froup-1-like protein structure-specific recognition protein-1(SSRP-1)의 이종단백질 복합체이다 본 논문에서는 FACT의 단위체인 hSpt16의 발현이 휴지기의 T98G 세포에서 급격히 감소됨을 면역형광 분석법과 Western blot 분석법을 이용하여 관찰하였다. 이와 반대로 증식기의 T98C 세포에서는 hSpt16이 높은 수준으로 발현되고 있는 것이 관찰되었다. FACT의 또 다른 단위체 SSRP-1의 발현은 휴지기나 증식기의 세포에서 변화가 없음이 관찰되었다. 이상의 결과로부터 hSpt16이 발현이 세포의증식과 연관이 되어 있음을 알 수 있었고 세포 증식 표지 인자로 사용될 수 있을 것으로 사료된다.

Keywords

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