Mycobiology

The Korean Society of Mycology (한국균학회)
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Detection of Extracellular Enzyme Activities in Various Fusarium spp

  • Kwon, Hyuk-Woo (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Yoon, Ji-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Cheon, Young-Ah (Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Ko, Seung-Ju (Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration)
  • Published : 2007.09.30
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Abstract

Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested species Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum showed high ${\beta}$-glucosidase acitivity. Xylanase activity was strongly detected in F. proliferatum and F. oxysporum. Strong pectinase activity was also found in F. oxysporum and F. proliferatum. Amylase activity was apparent in F. oxysporum. No clear activity in cellulase was found from all the Fusarium species tested.

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References

  1. Booth, C. 1977. Fusarium Laboratory Guide to the Identification of the Major Species. Commonweath Mycological Institute, Kew, 55 pp
  2. Castro, G. R., Baigori, M. D. and Sineriz, F. 1995. A plate technique for screening of inulin degrading microorganisms. J Microbiol. Methods 22: 51-56 https://doi.org/10.1016/0167-7012(94)00063-D
  3. Domsch, K. H., Gams, W. and Anderson, T.-H. 1980. Compendium of soil fungi. Academic Press, London
  4. Lee, S. T. and Lee, J. J. 1997. Insoluble dye substrate for screening and assay of xylan-degrading enzymes. J Microbiol. Methods 29: 1-5 https://doi.org/10.1016/S0167-7012(97)00986-X
  5. Nelson, P. E., Tousson, T. A. and Marasas, W. F. O. 1983. Fusarium species. An illustrated manual for identification. Pennsylvania State Univ Press, London
  6. Nwanma, B. N., Onyike and Nelson, P. E. 1993. The distribution of Fusarium species in soils planted to millet and sorghum in Lesotho, Nigeria and Zimbabwe. Mycopathologia 121: 105-114 https://doi.org/10.1007/BF01103578
  7. Teather, R. M. and Wood, P. J. 1982. Use of congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from bovine rumen. Appl. Environ. Microbiol. 43: 777-780
  8. Yoon, J. H. 2007. Study on the detection of extracellular enzyme activities in Korean isolates of Colletotrichum, Fusarium, Penicillium, and Trichoderma. MSc. Thesis, Dankook University, Cheonan, Korea
  9. Yoon, J. H., Park, J. E., Suh, D. Y., Hong, S. B., Ko, S. J. and Kim, S. H. 2007. Comparison of dyes for easy detection of extracellulase in fungi. Mycobiology 35: 21-24 https://doi.org/10.4489/MYCO.2007.35.1.021

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