Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Culture Media and Oxygen Concentration on In Vitro Development of Porcine IVM/IVE Embryos

배양액 및 산소농도가 돼지 체외수정란의 발달에 미치는 영향

  • Choe, C.Y. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Choe, S.R. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Choi, S.H. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Kim, H.J. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Han, M.H. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.) ;
  • Kang, D.W. (Department of Physiology, College of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Shin, Y.W. (Department of Physiology, College of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Han, J.H. (Department of Physiology, College of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Son, D.S. (Animal Genetic Resources Station, National Institute of Animal Science, R.D.A.)
  • 최창용 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 최선호 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 한만희 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 신용원 (경상대학교 의과대학 생리학교실) ;
  • 한재희 (경상대학교 의과대학 생리학교실) ;
  • 손동수 (농촌진흥청 축산과학원 가축유전자원시험장)
  • Published : 2007.09.29
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Abstract

During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen ($O_2$), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% $O_2$, in NCSU-23), the mean cell number per blastocyst was $40{\pm}10$. These results showed that medium and $O_2$ concentration had no significant effect on the development of porcine embryos.

본 연구는 돼지 체외수정란 생산효율을 향상시켜 돼지의 품종개량, 형질전환 돼지생산 등과 멸실위험에 처해 있는 유전자원의 보존을 위한 기술로 활용하기 위해 미성숙 난포란의 적정 체외성숙 시간을 알아보고, 배양액의 종류 및 체외 배양시의 산소 농도에 따른 체외수정란의 생산 효율을 확인한 결과는 다음과 같다. 1. 돼지 미성숙 난포란의 체외성숙 시간별 제2감수분열중기(M II)까지 성숙된 비율이 체외성숙 38, 40, 42시간째에 각각 61.1%, 42.9% 및 69.6%를 나타내어 그 비율이 70% 미만으로 낮은 반면, 체외성숙 44, 46, 48시간째에 각각 73.7%, 94.1% 및 100%의 체외성숙률을 나타내어 최소한 44시간 이상의 체외성숙이 필요한 것으로 조사되었다. 2. 체외배양액의 종류에 따른 배반포 발달률이 NCSU-23 18.8%, PZM-5 16.3%를 나타내어 유의적인 차이를 보이지 않았으며(p>0.05), 산소분압에 따른 배반포 발달률이 5% 산소분압에서 11.9%, 20%의 산소분압에서 15.8%를 나타내어 두 군간의 유의한 차이는 보이지 않았다(p>0.05). 3. 체외배양 7일째 발달된 배반포 수정란의 총세포수는 40개 내 외를 나타내었다. 이상의 결과, 돼지 미성숙 난포란의 체외성숙 시간은 44시간 정도가 적정한 것으로 확인되었으며, 서로 다른 배양액과 산소농도가 돼지 체외수정란의 배발달에 있어서 유의적인 차이를 나타내지는 못하였다.

Keywords

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