Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Expression of Two-pore Domain $K^+$ Channels in Endometrial Cells of Korean Cattle

한우의 자궁내막세포에서 발현되는 Two-pore Domain 포타슘 통로

  • Kang, D. (Department of Physiology, College of Medicine Gyeongsang National University) ;
  • Kim, E.S. (Department of Physiology, College of Medicine Gyeongsang National University) ;
  • Yang, H.Y. (Department of Physiology, College of Medicine Gyeongsang National University) ;
  • Choe, C.Y. (Animal Genetic Resources Station, National Institute of Animal Science, RAD) ;
  • Han, J. (Department of Physiology, College of Medicine Gyeongsang National University)
  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 김은숙 (경상대학교 의과대학 생리학교실) ;
  • 양혜영 (경상대학교 의과대학 생리학교실) ;
  • 최창용 (농촌진흥청 축산과학원 가축유전자원시험장) ;
  • 한재희 (경상대학교 의과대학 생리학교실)
  • Published : 2007.09.29
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Abstract

Endometrial cells play important roles in implantation and during pregnancy. This study was carried out to identify whether two-pore domain $K^+\;(K_{2P})$ channels are expressed in endometrial cells of Korean cattle. $K_{2P}$ channels set the resting membrane potential of many kinds of neuronal cells in the central and peripheral nervous systems. RT-PCR data showed that TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were expressed in bovine endometrial cells, and the mRNA expression levels were similar between endometrial cells with or without endometritis. The protein expression was confirmed by using commercially available polyclonal antibodies (TASK-3, TREK-1, TREK-2, and TRAAK). TASK-3 and TREK-1 were expressed in all area of endometrial cells including nuclei, while TREK-2 and TRAAK were expressed in all area of cells except nuclei. These results demonstrate for the first time the presence of $K_{2P}$ channel in endometrial cells of Korean cattle.

임신의 성립 및 유지에 중요한 역할을 하는 자궁내막세포에 $K_{2P}$ 통로의 존재를 확인하기 위하여 본 연구를 수행하였다. $K_{2P}$ 통로는 일반적으로 중추신경계에 풍부하게 존재하면서 세포의 안정막 전압을 유지시킨다. 역전사 중합 효소 중합 반응과 면역 세포 화학 염색 방법을 이용하여 자궁내막세포에 존재하는 $K_{2P}$ 통로를 조사한 결과, TASK-1, TASK-3, TREK-1, TREK-2 및 TRAAK의 발현이 확인되었다. TASK-3와 TREK-1은 핵을 포함한 세포 전역에 발현하였고, TREK-2와 TRAAK은 핵을 제외한 세포 전역에 발현하였다. 그러나 자궁내막염이 있는 자궁내막세포와 정상 자궁내막세포에서 $K_{2P}$ 통로의 발현 변화를 비교한 결과, 두 군간의 mRNA 발현 수준이 변화하지 않았다. 본 연구는 한우의 자궁내막세포에서 $K_{2P}$ 통로의 존재를 처음으로 보고하는데, 이를 통하여 한우의 자궁내막세포에서 확인된 $K_{2P}$ 통로들은 자궁의 생리 작용과 자궁암 등과 같은 여러 자궁 관련 질병에 관여할 가능성이 높을 것으로 생각된다.

Keywords

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