Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Charcterization of Micrococcus sp. HJ-19 Secreting Extracellular Protease

단백질 분해효소를 분비하는 Micrococcus sp. HJ19의 분리 및 특성

  • Cha, In-Tae (Department of Microbiology, Chungbuk National University) ;
  • Oh, Yong-Sik (Department of Microbiology, Chungbuk National University) ;
  • Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
  • 차인태 (충북대학교 자연과학대학 미생물학과) ;
  • 오용식 (충북대학교 자연과학대학 미생물학과) ;
  • 노동현 (충북대학교 자연과학대학 미생물학과)
  • Published : 2007.09.30
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Abstract

Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to screen new source of pretense, bacteria secreting extracellular pretense were isolated by enrichment culture from deep sea water samples of East Sea, Korea. A bacterium, named as HJ19, showed the best growth and the largest clear zone in plates supplemented skim milk at $30^{\circ}C$. The partial DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology identified that this strain was In genus Micrococcus. The strain HJ19 could not grow at $10^{\circ}C$ but it started growth and showed pretense activity at $20^{\circ}C$. The optimal growth was at $37^{\circ}C$ and the maximal protease activity at $30^{\circ}C$ was about 480unit/ml.

단백질 분해효소는 아미노산간에 존재하는 peptide결합을 절단하는 효소로 산업적으로 많이 사용된다. 이러한 단백질 분해효소의 새로운 공급원을 찾기 위하여 체외 단백질 분해효소를 생산하는 세균을 둥해 심층수로부터 집식배양으로 분리하였다. 이들 중 빠른 성장과 skim milk를 첨가한 배지에서 가장 큰 투명환을 보여준 균주를 HJ19로 명명하였다. 분리균의 형태학적, 생리생화학적 특성과 16S rRNA gene의 염기서열을 조사한 결과 Micrococcus sp.에 속하는 것으로 나타났다. 분리된 Micrococcus sp. HJ19는 $10^{\circ}C$에서는 자라지 못하였으며 $20^{\circ}C$부터 생육과 효소활성을 보여주었다. 최적 생육 온도는 $37^{\circ}C$였으며, 효소 활성은 $30^{\circ}C$에서 약 480 unit/ml의 활성을 보여 주었다.

Keywords

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