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Performance Evaluation of an Auto Sampling and Filtering Unit of Substrate Solution using a Diaphragm Pump

소형 판막 펌프를 이용한 기질용액 채취 및 여과 자동화 장치의 성능검증

  • Song, D.B. (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science) ;
  • Jung, H.S. (Division of Agricultural Engineering, Gyeongsang National University) ;
  • Lee, S.K. (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science) ;
  • Jung, D.H. (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science) ;
  • Park, S.W. (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science)
  • Published : 2007.08.25

Abstract

An auto sampling and filtering unit was developed for monitoring automation of a fermentation process and its performance was evaluated. The automatic sampling and filtering unit was constructed with a glass filter, a diaphragm suction pump, and a flow direction change valve. To evaluate operating stability, delivery volumes of the suction pump were measured according to the experimental conditions of cellulose powder, pore size of the glass filter and suction head of the pump. The developed unit could deliver the sample solution under any experimental conditions except the filter pore size of $16{\mu}m$ and the suction head of 20cm. In case of the suction head of 30cm, the pump could not deliver the sample solution at all. Concentrations of the sample solutions were converged on those of the standard glucose solution after 8 minutes from the initial sampling time. The relative error of concentration between the sample and the standard solution showed 3.8, 4.8, 7.0% for the 1, 3, 5% contents of cellulose powder, respectively.

Keywords

References

  1. Merbel, N. C., H. Lingeman and U. A. Brinkman. 1993. Membrane-based sample preparation for chromatography. Journal of Chromatography 634:1-29 https://doi.org/10.1016/0021-9673(93)80308-U
  2. Mattiasson, B. and H. Hakanson. 1993. Sampling and sample handling-crucial steps in process monitoring and contro l. TIBTECH. 11:136-142 https://doi.org/10.1016/0167-7799(93)90088-Q
  3. Picque, D. and G. Corneu. 1992. Performance of aseptic sampling devices for on-line monitoring of fermentation processes. Biotech. & Bioeng. 40:919-924 https://doi.org/10.1002/bit.260400808
  4. Rocha, I. and E. C. Ferreira. 2002. On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Analytica Chimica Acta. 462:293-304 https://doi.org/10.1016/S0003-2670(02)00347-1