International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Characterization of Small Heat Shock Protein(hsp20.8A) from the Silkworm, Bombyx mori

  • Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Go, Hyun-Jeong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Goo, Tae-Won (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Seong, Su-Il (Department of Life Science, College of Natural Science, University of Suwon) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Kwan-Ho (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Ik-Soo (College of Agriculture & Life science, Chonnam National University) ;
  • Jeon, Jae-Pil (Korea National Institute of Health) ;
  • Kang, Seok-Woo (Department of Life Science, College of Natural Science, University of Suwon)
  • Published : 2007.09.30
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Abstract

To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp20.8A, which was expressed at a high level in diapausing eggs that had been incubated at $25^{\circ}C$ for 30 days after oviposition, whereas, in the eggs exposed to $15^{\circ}C$ for 30 days, $5^{\circ}C$ for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 days after heat-HCl treatment and later at 9 to 10 days after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.

Keywords

References

  1. Chen, J. H., T. Yaginuma and O.Yamashita (1989) Changes in cyclic AMP and cyclic GMP content related to embryogene- sis and diapause of the silkworm, Bombyx mori. J. Seric. Sci. Jpn. 58(1),60-65
  2. Furusawa T, A. Konishi, D. Sakano, E. Kotani, Y. Sugimura, J. M. Storey and K. Storey (1999) Regulation properties of phosphofructokinase in the eggs of silkworm, Bombyx mori. J. Insect Physiol. 46, 1009-1016 https://doi.org/10.1016/S0022-1910(99)00212-7
  3. Horie Y, T. Kanda and Y. Mochida (2000) Sorbitol as an arrester of embryonic development in diapausing eggs o fthe silkworm, Bombyx mori. J. Insect physiol. 46, 1009-1016 https://doi.org/10.1016/S0022-1910(99)00212-7
  4. Hwang, J. S., H. J. Go, T. W.Goo, E. Y. Yun, K. H. Choi, S. I. Seong, S. M. Lee, B. H. Lee ,I. Kim, T. Chun and S. W. Kang (2005) The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori. Archives of Insect Biochemistry and Physiology 59, 197-201 https://doi.org/10.1002/arch.20057
  5. Iwasaki H., M. Takahashi, T. Niimi, O. Yamashita and T. Yaginuma (1997) Cloning the cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs. Insect Mol. Biol. 6(3),131-141 https://doi.org/10.1111/j.1365-2583.1997.tb00081.x
  6. Li, B., Q. Y. Xia, H. Fujii and Y. Banno (2000) Isolation of small heat-shock genes (hsp20.8A) from the silkworm, Bombyx mori. GenBank accession number AF315319
  7. Maruyama K. and S. Sugano (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138, 171-174 https://doi.org/10.1016/0378-1119(94)90802-8
  8. Moribe Y, T. Niimi, O. Yamashita and T. Yaginuma (2001) Samui, a novel cold inducible gene, encoding a protein with a BAG domain similar to silencer of death domain(SODD/ BAG-4), isolated from Bombyx diapause eggs. Eur. J. Biochem. 268(12), 3432-42 https://doi.org/10.1046/j.1432-1327.2001.02244.x
  9. Rinehart J. P. and D. L. Denlinger (2000) Heat shock protein 90 is downregulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress. Insect Mol. Biol. 9,641-45 https://doi.org/10.1046/j.1365-2583.2000.00230.x
  10. Rinehart J. P., G D. Yocum and D. L. Denlinger (2000) Developmental upregulation of inducible hsp70 transcripts, but not the cognate form, during pupal diapause in the flesh fly, Sarcophaga crassipalpis. Insect Biochem. Mol. Biol. 30, 515-21 https://doi.org/10.1016/S0965-1748(00)00021-7
  11. Sato Y.,M. Oguchi, N. Menjo, K. Imai, H. Saito, M. Ikeda, M. Isobe and O. Yamashita (1993) Precursor polyprotein for multiple neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori: characterization of the cDNA encoding the diapause hormone precursor and identification of additional peptides. Proc.Natl. Acad. Sci. U. S. A. 90,3251-3255
  12. Suzuki M.G., T. Terada, M. Kobayashi and T. Shimada (1999) Diapause-associated transcription ofBmEts, a gene encoding an ETS transcription factor homolog in Bombyx mori. Insect Biochem. Mol. Biol. 29(4),339-47 https://doi.org/10.1016/S0965-1748(99)00008-9
  13. Tani N, M. Isobe, S. Suwan and H. Kai (2002) PIN peptide, corresponding to fragments of egg-specific protein, interacts with EA4 to inhibit deglycosylation by PNGase F. J. insect Biotech. Seric. 71,97-102
  14. Tani N, T. Kamada, K. Ochiai, M. Isobe, S. Suwan and H. Kai (2001) Carbohydrate moiety of time-interval measuring enzyme regulaters time measurement through its interaction with time-holding peptide PIN. J. Biochem. 129,221-227 https://doi.org/10.1093/oxfordjournals.jbchem.a002848
  15. Xu W. H., Y. Sato, M. Ikeda and O, Yamashita (1995) Molecular characterization of the gene encoding the precursor protein of diapause hormone and pheromone biosynthesis activating neuropeptide (DH-PBAN) of the silkworm, Bombyx mori and its distribution in some insects. Biochemica et Biophysica Acta 1261, 83-89 https://doi.org/10.1016/0167-4781(94)00238-X
  16. Yaginuma T and O Yamashita (1986) Malate-aspartate cycle as an effective hydrogen shuttle at the termination of diapause in the eggs of Bombyx mori. Insect Biochem. 16, 677-685 https://doi.org/10.1016/0020-1790(86)90011-9
  17. Yaginuma T and O. Yamashita (1999) Oxygen consumption in relation to sorbitol utilization at the termination of diapause in eggs of the silkworm, Bombyx mori. J. Insect. Physiol. 45, 621-627 https://doi.org/10.1016/S0022-1910(98)00150-4
  18. Yamashita O. and K. Hasekawa (1985) Embryonic diapause. In comparative insect physiology, Biochemistry and pharmacology(edited by kerkut GA and Gillbert L1) Vol. 1, Pergamone Press. Oxford, 407-434
  19. Yocum G. D., K. H. Joplin and D. L. Denlinger (1998) Upregulation of a 23 kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis. Insect Biochem. Mol. Biol. 28,677-82 https://doi.org/10.1016/S0965-1748(98)00046-0