International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Egg Diapause Induction in Multivoltine Silkworm Bombyx mori for Long-term Germplasm Preservation

  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

At present, multivoltine silkworm races reared five times per year involving huge manpower and rearing expenditure. Egg diapausing behavior is facultative in multivoltine and egg diapause was induced in selected multivoltine races by regulating temperature at $18^{\circ}C$, relative humidity 80% and photoperiod (6L:18D) in the late stage silkworm rearing. The maximum percentage of egg diapause induction was recorded in Rong Diazo, Diazo and MW13 showed 94%, 93% and 92% respectively, whereas the races A14DY and OS-616 showed minimum diapause induction 15% and 18% respectively. The diapause induced multivoltine eggs were preserved up to six months by cold preservation schedule normally adopted for bivoltine. After three and six months egg preservation, the diapause induced layings were released and observed for hatching percentage, all races showed above 82 % of hatching except the race AP12, which showed only 78 % of hatching. This methods reduce the crop cycle, gives strong safety backup and preventing the genetic erosion. This study helps formulating a new conservation method for multivoltine silkworm germplasm.

Keywords

References

  1. Denlinger, D.L. (1985) Hormonal control of diapause. In Comprehensive Insect Physiology Biochemistry and Pharmacology (Eds Kerkut G. A. and Gilber L. I.), Vol. 8, pp. 353-412. Pergamon Press, Oxford
  2. Jayaswal, K.P. (1996) Impact of diapause on silk production in Bombyx mori L. Ph.D. Thesis, University of Calcutta. pp. 1-80
  3. Jayaswal, K.P. (2001) Isolation of the lines with different dia- pause tendencies and its influence on quantitative traits in the tropical multivoltine breed of Bombyx mori L. Indian J. Seric. 40(2), 127-133
  4. Kogure, M. (1933) The influence of light and temperature on certain characters of the silkworm, Bombyx mori. Dept. Agri. Kyushu Imp. Univ., 4,1-93
  5. Krishnaswami, S. (1978) New Technology of Silkworm Rearing. Bulletin No.2., Central Sericultural Research and Training Institute, Mysore, Central Silk Board, Govt. of India
  6. Kumaresan, P., K. Thangavelu and R. K. Sinha (2004) Studies on long-term preservation of eggs of Indian tropical multivoltine silkworm (Bombyx mori L.) genetic resources. Int. J Indust. Entomol. 9(1), 79-87
  7. Kobayashi, J., H. Ebinuma and M. Kobayashi (1986) Effect of temperature on the diapause egg production in the tropical race of the silkworm, Bombyx mori. J. Seric. Sci. Jpn., 55 (4),343-348
  8. Lees, A. D. (1955) The Physiology of Diapause in Arthropods. Cambridge Univ. Press, Cambridge
  9. Morohoshi, S. (1979) Development Physiology of Silkworms. (Translation of 2nd Japanese Edition, 2000), Oxford & IBH Publishing Co., New Delhi. 1-289
  10. Murakami, A. (1989) Genetic studies on tropical races of silkworm (Bombyx mori L.) with special reference to cross breeding strategy between tropical and temperate races. 2. Multivoltine silkworm strains in Japan and their origin. JARQ, 23(2), 127- 133
  11. Nagayama, K. and T. Yamamoto (1987) Effect of low temperature during 4th, 5th larval and pupal stages on the diapause in several multivoltine silkworm strains. J. Seric. Sci. Jpn., 56 (2),247-248
  12. Radhakrishnan, S., P. Kumaresan, R.K. Sinha and K. Thangavelu (2003) Induction of diapause in the eggs of multivoltine silkworm (Bombyx mori) germplasm for long-term preservation. Proc. Natl. Semi. Silkworm Seed prodn, SSTL, Bangalore. 155-158
  13. Saunders, D. S. (1982) Insect Clocks, 2nd edn, pp. 137-192. Pergamon Press, Oxford
  14. Suresh kumar, N., H.K. Basavaraja, G V. Kalpana, N. Malreddy, K.P. Jayaswal, T. Thippeswamy and R.K. Datta (2000) Cocoon filament size deviation in bivoltine silkworm, Bombyx mori L. Indian J. Seric 41(1), 42-48
  15. Tazima, Y. (1978) The silkworm: an important laboratory tool. Kodansa Public. Tokyo, Japan