Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Antitumor Effects of Kluyveromyces marxianus TFM-7 Isolated from Kefir

  • Lee, Hyun-Jung (Division of Animal Life Science, Konkuk University) ;
  • Nam, Bo-Ra (Division of Animal Life Science, Konkuk University) ;
  • Kim, Jin-Man (Division of Animal Life Science, Konkuk University) ;
  • Kim, Ji-Yeon (Department of Animal Science, Woosong Information College) ;
  • Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University) ;
  • Kim, Chang-Han (Division of Animal Life Science, Konkuk University)
  • Published : 2007.02.28
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Abstract

The Strain TFM-7, Which has an antitumor effect, was isolated from Kefir and identified based on analysis using the API 50 CHL kit and 265 rDNA sequencing. Strain TFM-7 was confirmed to belong to the genus Kluyveromyces. Analysis of the 265 rDNA nucleotide sequences found strain TFM-7 to be related to Kluyveromyces marxianus. NRRL Y-828IT. K. marxianus. TFM-7 was cultured with potato dektrose broth medium at $27^{\circ}C$ for 72 hr, and its inhibition effects on the proliferation of seven tumor cell lines and a normal cell line were assessed using the MTT assay. The antitumor effects and growth characteristics of K. marxianus TFM-7 were investigated during a culture period of 7 days. By the $3^{rd}\;day$, K. marxianus TFM-7 showed a dry cell weight 2.39 g/L, a pH of 4.39, an ethanol content of 0.89%, and an inhibition effect on the proliferation of seven tumor cell lines above 50%, except for A-549 tumor cell line. K. marxianus TFM-7 was the most effective at inhibiting the growth of Hep-2 cell line among all tumor cell lines tested. Growth inhibition of a normal cell line, NIH/3T3, was less than 35%, suggesting a decreased level of cytotoxicity toward normal cells. These results indicate that K. marxianus TFM-7 may have used as a yeast strain with antitumor activity.

Keywords

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