Rapid Prediction of Amylose Content of Polished Rice by Fourier Transform Near-Infrared Spectroscopy

  • Lee, Jin-Cheol (Biotechnology Industrialization Center, Dongshin University) ;
  • Yoon, Yeon-Hee (Biotechnology Industrialization Center, Dongshin University) ;
  • Kim, Sun-Min (Biotechnology Industrialization Center, Dongshin University) ;
  • Pyo, Byong-Sik (Biotechnology Industrialization Center, Dongshin University) ;
  • Hsieh, Fu-Hung (Biological Engineering Department, University of Missouri) ;
  • Kim, Hak-Jin (National Institute of Agricultural Engineering, Rural Development Administration) ;
  • Eun, Jong-Bang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
  • Published : 2007.06.30


Fourier transform near-infrared (FT-NIR) spectroscopy and partial least squares (PLS) regression were used to predict the amylose content of polished rice. Spectral reflectance data in a wavelength range of 1,000 to 2,500 nm were obtained with a commercial spectrophotometer for 60 different varieties of Korean rice. For a comparison of this spectroscopic method to a standard chemical analysis, the amylose contents of the tested rice samples were determined by the iodine-blue colorimetric method. The highest correlation for the rice amylose ($R^2=0.94$, standard error of prediction=0.20% amylose content) was obtained when using the FT-NIR spectrum data pre-treated with normalization, the first derivative, smoothing, and scattering correction.


  1. Chi HY, Lee CH, Kim KH, Kim, SL, Chung IM, Induction of apoptopic cell death by red pericarp rice (Jakwangchalbyeo) extracts. Food Sci. Biotechnol. 15: 534-542 (2006)
  2. Kim SS, Kim DC. Quality of rice marketed in Korea. Food Sci. Biotechnol. 7: 255-228 (1998)
  3. Chae JC, Jung MS, Jun DK, Son YM. Relationship between yield and quality of rice varieties grown in reclaimed saline paddy field. Korean J. Crop Sci. 47: 259-262 (2002)
  4. Son JR, Kim JH, Lee, JI, Youn YH, Kim JK, Hwang HG, Moon HP. Trend and further research of rice quality evaluation. Korean J. Crop Sci. 47: 33-54 (2002)
  5. Chae JC. Present situation, research, and prospect of rice quality and bioactivity in Korea. Food Sci. Ind. 37: 47-54 (2004)
  6. Moon SS, Lee KH, Cho RK. Application of near-infrared reflectance spectroscopy in quality evaluation of domestic rice. Korean J. Food Sci. Technol. 26: 718-725 (1994)
  7. Hwang HG, Cho RK, Sohn JK, Lee SK. Rapid evaluation of chemical components of rice grain using near infrared spectroscopy. Korean J. Crop Sci. 39: 7-14 (1994)
  8. Delwiche SR, Mckenzie KS, Webb BD. Quality characteristics in rice by near-infrared reflectance analysis of whole-grain samples. Cereal Chem. 73: 257-263 (1996)
  9. Kim YS, Scotter C, Voyiagis M, Hall M. Potential of NIR spectroscopy of discriminating geographical origin of green tea from Korea and Japan. Food Sci. Biotechnol. 6: 74-78 (1997)
  10. Kim SK, Chae JC, Lim MS, Ree JH. Interrelationship between amylose content and physical properties of milled rice. Korean J. Crop Sci. 30: 320-325 (1985)
  11. Kim KH, Kim DH, Cho YH. Development of near isogenic lines and genetic analysis for amylose content of rice grain. Korean J. Breed. 33: 280-286 (2001)
  12. Lee SH, Han O, Lee HY, Kim SS, Chung DH. Physicochemical properties of rice starch by amylose content. Korean J. Food Sci. Technol. 21: 766-771 (1989)
  13. Czuchajowska Z, Szczodrak J, Pomeranz Y. Characterization and estimation of barley polysaccharides by near-infrared spectroscopy. I. Barleys, starches, and $\beta$-D-glucans. Cereal Chem. 69: 413-418 (1992)
  14. Osborne BG, Fearn T. Theory of near-infrared spectrophotometry. pp. 13-35. In: Near Infrared Spectroscopy in Food Analysis. Osborne BG, Fearn T (eds). Longman Scientific and Technical, Essex, UK (1986)
  15. McClure WF, Maeda H, Dong J, Liu Y, Ozaki Y. Two-dimensional correlation of Fourier transform near-infrared and Fourier transform Raman spectra. I: Mixtures of sugar and protein. Appl. Spectrosc. 50: 467-475 (1996) https://doi.org/10.1366/0003702963906177
  16. Chung HI, Kim HJ. Near-infrared spectroscopy: principles. Anal. Sci. Technol. 13: 1A-14A (2000)
  17. Miyamoto K. Classification of high acid fruits by PLS using the near infrared transmittance spectra of intact satsuma mandarins. J. Near Infrared Spec. 6: 267-271 (1998) https://doi.org/10.1255/jnirs.146
  18. Juliano BO. Criteria and tests for rice grain qualities. Rice Chem. Anal. Technol. 55: 443-524 (1985)
  19. Juliano BO. A simplified assay for milled-rice amylose. Cereal Sci. Today 16: 334-338 (1971)
  20. Kim SK, Jung SO. Physicochemical properties of Japonica nonwaxy and waxy rice during kernel development. Food Sci. Biotechnol. 15: 289-297 (2006)
  21. Reeves JB. Discriminant analysis of selected food ingredients by near infrared diffuse reflectance spectroscopy. J. Near Infrared Spec. 5: 209-221 (1997) https://doi.org/10.1255/jnirs.114
  22. ASTM. American Society of Testing and Materials Annual Book of Standards. Vol. 03.06, Doc. E1655-94. Standard practices for infrared, multi-variate, quantitative analysis. American Society of Testing and Materials, West Conshohochen, PA, USA (1995)
  23. Cho HJ, Ha YL. Determination of honey quality by near infrared spectroscopy. Korean J. Food Sci. Technol. 34: 356-360 (2002)
  24. Kim JM, Choi CH, Min BK, Kim JH. Development of prediction model for moisture and protein content of single kernel rice using spectroscopy. J. Korean Soc. Agric. Machinery 23: 49-56 (1998)
  25. Lee SM, Kim S, Park JB, Hwang IK. Prediction of chemical compositions for on-line quality measurement of red pepper powder using near infrared reflectance spectroscopy (NIRS). Food Sci. Biotechnol. 14: 280-285 (2005)
  26. Sohn MR, Cho RK. Possibility of nondestructive evaluation of pectin in apple fruit using near-infrared reflectance spectroscopy. J. Korean Soc. Hort. Sci. 41: 65-70 (2000)
  27. Norris KH, Williams PC, Optimization of mathematical treatments of raw near-infrared signal in the measurement of protein in hard red spring wheat. I. Influence of particle size. Cereal Chem. 61: 158-165 (1984)