Antioxidant Activities of Ethanol Extracts from Germinated Specialty Rough Rice

  • Lee, Youn-Ri (Department of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan-Sik (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Kee-Jong (Crop Post-Harvest Technology Division, National Institute of Crop Science, Rural Development Administration) ;
  • Son, Jong-Rok (Crop Post-Harvest Technology Division, National Institute of Crop Science, Rural Development Administration) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2007.10.31

Abstract

To examine the possibility of using rough rice as a health-functional food, we investigated its changes in biological activity with germination. Antioxidant activities of the 70% ethanolic extracts of 'Goami2', 'Keunnunbeyo', and 'Heugkwangbeyo' were studied in comparison with those of ungerminated rough rice. The phytic acid level in rough rice decreased on germination, while the level of phenolic compounds increased. Reducing power increased in a dose-dependent manner and the germinated rough rice tended to have enhanced reducing power. Among the rough rice cultivars, the germinated 'Heugkwang' rough rice tended to be the most effective, with scavenging activities for the DPPH, superoxide, and hydroxyl radicals that were 1.6-, 1.3-, and 1.6-fold greater than those of the ungerminated 'Heugkwang' rough rice, respectively. We also found that the germination process increased antioxidant activity in all the rough rice cultivars, where activity was greatest for the 'Heugkwang' rough rice cultivar.

Keywords

References

  1. Amarowicz R, Pegg RB, Barl B, Weil JA. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 84: 551-562 (2004) https://doi.org/10.1016/S0308-8146(03)00278-4
  2. Larrauri JA, Ruperez P, Saura CF. Effect of drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels. J. Agr. Food Chem. 45: 1390-1393 (1997) https://doi.org/10.1021/jf960282f
  3. Halliwell B, Murcia MA, Chirico S, Aruoma Ol. Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit. Rew. Food Sci. 35: 7-13 (1995) https://doi.org/10.1080/10408399509527682
  4. Heo SJ, Park PJ, Park EJ, Cho SM, Kim SK, Jeong YJ. Antioxidative effect of proteolytic hydrolysates from Ecklonia cava on radical scavenging using ESR and $H_2O_2$-induced DAN damage. Food Sci. Biotechnol. 14: 614-620 (2005)
  5. Stadtman ER. Protein oxidation and aging. Science 257: 1220-1224 (1996) https://doi.org/10.1126/science.1355616
  6. Milner A. Functional foods and health promotion. J. Nutr. 129: 1395-1397 (1999) https://doi.org/10.1093/jn/129.7.1395S
  7. Kaur C, Kapoor HC. Antioxidants in fruits and vegetables the millennium's health. Int. J. Food Sci. Technol. 36: 703-725 (2001) https://doi.org/10.1046/j.1365-2621.2001.00513.x
  8. Rimsten L, Haraldsson AK, Andersson R, Alminger M, Sandberg AS, Aman P. Effects of malting on beta-glucans and phytase activity in barley grain. J. Sci. Food Agr. 82: 904-912 (2003) https://doi.org/10.1002/jsfa.1135
  9. Yuan HL, Yeh CS. Extrusion processing of rice-based breakfast cereals enhanced with tocopherol from a Chinese medical plant. Cereal Chem. 80: 491-494 (2003) https://doi.org/10.1094/CCHEM.2003.80.4.491
  10. Mikola M, Brinck O, Jones BL. Characterization of oat endoproteinases that hydrolyze oat avenins. Cereal Chem. 78: 55-58 (2001) https://doi.org/10.1094/CCHEM.2001.78.1.55
  11. Beal AD, Mottram DS. An evaluation of the aroma characteristics of malted barley by free-choice profiling. J. Sci. Food Agr. 61: 17- 22 (1993) https://doi.org/10.1002/jsfa.2740610104
  12. Subba R, Muralikrishna G. Evaluation of the antioxidant properties of free and bound phenolic acids from native and malted finger millet. J. Sci. Food Agr. 50: 889-892 (2002) https://doi.org/10.1021/jf011210d
  13. Heinio RL, Oksman KM, Latva K, Lehtinen P, Poutanen K. Effects of drying treatment conditions on sensory profile of germinated oat. Cereal Chem. 78: 707-714 (2001) https://doi.org/10.1094/CCHEM.2001.78.6.707
  14. Karppinen S, Liukkonen K, Aura AM, Forssell P, Poutanen K. In vitro fermentation of polysaccharides of rye, wheat, and oat brans and inulin by human faecal bacteria. J. Sci. Food Agr. 80: 1469- 1476 (2000) https://doi.org/10.1002/1097-0010(200008)80:10<1469::AID-JSFA675>3.0.CO;2-A
  15. Woo SM, Jeong YJ. Effect of germinated brown rice concentrates on free amino acid levels and antioxidant and nitrite scavenging activity in kimchi. Food Sci. Biotechnol. 15: 351-356 (2006)
  16. Juana F, Martha L, Miranda RD, Concepcion V. Effect of germination and fermentation on the antioxidant vitamin content and antioxidant capacity of Lupinus albus L. var. Multolupa. Food Chem. 92: 211-220 (2005) https://doi.org/10.1016/j.foodchem.2004.06.049
  17. Murata T, Akazawa T, Fukuchi S. Enzymatic mechanism of starch breakdown in germinating rice seeds. Plant Physiol. 43: 1899-1905 (1968) https://doi.org/10.1104/pp.43.12.1899
  18. Yasumatsu K, Moritaka S. Fatty acid composition of rice lipids and their change during storage. Agr. Biol. Chem. Tokyo 28: 257-261 (1964) https://doi.org/10.1271/bbb1961.28.257
  19. Haung W, Lantzsch HJ. Sensitive method for the rapid determination of phytate in cereals and cereal products. J. Sci. Food Agr. 34: 1423-1426 (1983) https://doi.org/10.1002/jsfa.2740341217
  20. Yu L, Perret J, Harris M, Wilson J, Haley S. Antioxidant properties of bran extracts from 'Akron' wheat grown at different locations. J. Agr. Food Chem. 51: 1566-1570 (2003) https://doi.org/10.1021/jf020950z
  21. Oyaizu. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44: 307-315 (1986) https://doi.org/10.5264/eiyogakuzashi.44.307
  22. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agr. Food Chem. 43: 27-32 (1995) https://doi.org/10.1021/jf00049a007
  23. Halliwell B, Gutteridge JM, Grootveld M. Methods for the measurement of hydroxyl radicals in biochemical systems: deoxyribose degradation and aromatic hydroxylation. Method Biochem. Anal. 33: 59-90 (1987)
  24. Aruoma OI, Halliwell B, Dizdaroglu M. Iron independent modification of bases in DNA by the superoxide radical generating system hypoxanthine/xanthine oxidase. J. Biol. Chem. 264: 13024-13030 (1989)
  25. Park KB, Oh SH. Production and characterization of GABA rice yogurt. Food Sci. Biotechnol. 14: 518-522 (2005)
  26. Sandberg AS, Carlsson NG, Svanberg U. Effects of inositol tri-, tetra-, penta-, hexaphosphates on in vitro estimation of iron availability. J. Food Sci. 54: 159-186 (1989) https://doi.org/10.1111/j.1365-2621.1989.tb08591.x
  27. Mandal NS, Burman BM. Isolation purification and characterization of phytase from germinating mungbeans. Phytochemisty 11: 495- 502 (1972) https://doi.org/10.1016/0031-9422(72)80003-7
  28. Zhow JR, Erdman JW. Phytic acid in health and disease. Crit. Rev. Food Sci. Nutr. 35: 495-504 (1995) https://doi.org/10.1080/10408399509527712
  29. Shahidi F, Wansundara PK. Phenolic antioxidant. Crit. Rew. Food Sci. 32: 67-103 (1992) https://doi.org/10.1080/10408399209527581
  30. Ayunmi H, Masatsune M. Analysis of free and bound phenolics in rice. Food Sci. Technol. Res. 5: 74-79 (1999) https://doi.org/10.3136/fstr.5.74
  31. Bunzel M, Allerdings E, Sinwall V. Cell wall hydroxycinnamates in wild rice in soluble dietary fibre. Eur. Food Res. Technol. 214: 482- 488 (2002) https://doi.org/10.1007/s00217-002-0512-3
  32. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agr. Food Chem. 43: 1813-1815 (1995) https://doi.org/10.1021/jf00055a012
  33. Shimada K, Fijikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthine on the autoxidation of soybean oil in cyclodextrin emulsions. J. Agr. Food Chem. 40: 945-948 (1992) https://doi.org/10.1021/jf00018a005
  34. Duh PD. Antioxidant activity of Budrock (Arctium laooa Linn) its scavenging effect on free radical and active oxygen. J. Am. Oil Chem. Soc. 75: 455-461 (1998) https://doi.org/10.1007/s11746-998-0248-8
  35. Isabel C, Ferreira PB, Miguel V, Lillian B. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stripe activity. Food Chem. 100: 1511-1516 (2007) https://doi.org/10.1016/j.foodchem.2005.11.043
  36. Pyo YH, Lee TC, Logendra L, Rosen RT. Antioxidant activity and phenolic compounds of Swiss chard extracts. Food Chem. 85: 19- 26 (2004) https://doi.org/10.1016/S0308-8146(03)00294-2
  37. Bloknina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage, and oxygen deprivation stress. Ann. Bot. London 91: 179- 194 (2003) https://doi.org/10.1093/aob/mcf118
  38. Hochestein P, Atallah AS. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat. Res. 202: 363-375 (1988)